NADK (NM_023018) Human Tagged ORF Clone

CAT#: RC200544

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NADK (Myc-DDK-tagged)-Human NAD kinase (NADK), transcript variant 1



  "NM_023018" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 118.00

USD 616.00


Availability*
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Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol NADK
Synonyms dJ283E3.1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC200544 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAATGGAACAAGAAAAAATGACCATGAATAAGGAATTGAGTCCAGACGCGGCTGCTTACTGCTGCT
CGGCCTGCCACGGCGATGAGACCTGGAGTTACAACCACCCCATCCGGGGCCGGGCCAAGTCTCGCAGCCT
GTCTGCCTCGCCCGCCCTGGGGAGCACCAAGGAGTTCAGGAGGACACGCTCTCTTCATGGGCCATGCCCG
GTGACCACTTTTGGACCAAAGGCCTGTGTGCTGCAGAACCCCCAGACCATCATGCACATTCAGGACCCCG
CGAGCCAGCGGCTGACGTGGAACAAGTCCCCAAAGAGCGTCCTTGTCATCAAGAAGATGAGAGATGCCAG
CCTACTGCAGCCGTTCAAGGAGCTCTGCACGCACCTCATGGAGGAGAACATGATCGTGTATGTGGAAAAG
AAAGTGCTAGAAGACCCTGCCATCGCCAGCGATGAAAGCTTTGGGGCAGTGAAGAAGAAATTCTGTACCT
TTCGAGAAGATTATGATGACATTTCCAATCAGATAGACTTCATCATCTGCCTGGGGGGAGACGGGACGCT
GCTGTACGCTTCCTCGCTTTTCCAGGGCAGCGTCCCTCCGGTCATGGCCTTCCACCTGGGCTCCCTGGGC
TTCCTGACCCCATTCAGCTTTGAGAACTTTCAGTCCCAAGTTACTCAGGTGATAGAGGGGAACGCAGCTG
TTGTTCTCCGGAGTCGGCTGAAGGTCAGGGTGGTGAAGGAGCTCCGGGGGAAGAAGACGGCCGTGCACAA
TGGGCTGGGTGAGAAAGGCTCGCAGGCTGCAGGCCTGGACATGGATGTCGGGAAGCAGGCCATGCAGTAC
CAGGTCCTGAATGAGGTGGTGATTGACAGAGGCCCCTCCTCCTACCTGTCCAATGTGGATGTCTACCTGG
ACGGACACCTCATCACCACGGTGCAGGGCGACGGAGTGATCGTGTCCACCCCGACGGGCAGCACGGCGTA
TGCGGCCGCGGCCGGGGCCTCCATGATCCACCCCAACGTGCCGGCCATCATGATCACGCCCATCTGCCCC
CACTCGCTGTCCTTCCGGCCCATCGTGGTCCCCGCAGGGGTCGAGCTGAAGATCATGCTGTCACCTGAAG
CAAGGAACACAGCATGGGTGTCCTTTGATGGACGGAAGAGACAAGAGATCCGCCATGGAGACAGCATCAG
CATCACTACCTCATGCTACCCGCTCCCCTCCATCTGTGTGCGGGACCCCGTGAGCGACTGGTTTGAGAGC
CTCGCCCAGTGCCTGCATTGGAACGTCCGGAAGAAGCAAGCCCACTTCGAGGAGGAGGAGGAGGAGGAGG
AGGAGGGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC200544 protein sequence
Red=Cloning site Green=Tags(s)

MEMEQEKMTMNKELSPDAAAYCCSACHGDETWSYNHPIRGRAKSRSLSASPALGSTKEFRRTRSLHGPCP
VTTFGPKACVLQNPQTIMHIQDPASQRLTWNKSPKSVLVIKKMRDASLLQPFKELCTHLMEENMIVYVEK
KVLEDPAIASDESFGAVKKKFCTFREDYDDISNQIDFIICLGGDGTLLYASSLFQGSVPPVMAFHLGSLG
FLTPFSFENFQSQVTQVIEGNAAVVLRSRLKVRVVKELRGKKTAVHNGLGEKGSQAAGLDMDVGKQAMQY
QVLNEVVIDRGPSSYLSNVDVYLDGHLITTVQGDGVIVSTPTGSTAYAAAAGASMIHPNVPAIMITPICP
HSLSFRPIVVPAGVELKIMLSPEARNTAWVSFDGRKRQEIRHGDSISITTSCYPLPSICVRDPVSDWFES
LAQCLHWNVRKKQAHFEEEEEEEEEG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_023018
ORF Size 1338 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_023018.5
RefSeq Size 3244 bp
RefSeq ORF 1341 bp
Locus ID 65220
UniProt ID O95544
Cytogenetics 1p36.33
Domains NAD_kinase
Protein Families Druggable Genome
Protein Pathways Metabolic pathways, Nicotinate and nicotinamide metabolism
MW 49.2 kDa
Gene Summary NADK catalyzes the transfer of a phosphate group from ATP to NAD to generate NADP, which in its reduced form acts as an electron donor for biosynthetic reactions (Lerner et al., 2001 [PubMed 11594753]).[supplied by OMIM, Mar 2008]

Other Versions

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