HEXIM1 (NM_006460) Human Tagged ORF Clone

CAT#: RC200536

HEXIM1 (Myc-DDK-tagged)-Human hexamethylene bis-acetamide inducible 1 (HEXIM1)

Product Images

Circular map for RC200536

• 

  Western blot validation of overexpression lysate (Cat# LY416644) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200536 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HEXIM1
• Synonyms: CLP1; EDG1; HIS1; MAQ1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200536 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGAGCCATTCTTGTCAGAATATCAACACCAGCCTCAAACTAGCAACTGTACAGGTGCTGCTGCTG
  TCCAGGAAGAGCTGAACCCTGAGCGCCCCCCAGGCGCGGAGGAGCGGGTGCCCGAGGAGGACAGTAGGTG
  GCAATCGAGAGCGTTCCCCCAGTTGGGTGGCCGTCCGGGGCCGGAGGGGGAAGGGAGCCTGGAATCCCAA
  CCACCTCCCTTGCAGACCCAGGCCTGTCCAGAATCTAGCTGCCTGAGAGAGGGCGAGAAGGGCCAGAATG
  GGGACGACTCGTCCGCTGGCGGCGACTTCCCGCCGCCGGCAGAAGTGGAGCCGACGCCCGAGGCCGAGCT
  GCTCGCCCAGCCTTGTCATGACTCCGAGGCCAGTAAGTTGGGGGCTCCTGCCGCAGGGGGCGAAGAGGAG
  TGGGGACAGCAGCAGAGACAGCTGGGGAAGAAAAAACATAGGAGACGCCCGTCCAAGAAGAAGCGGCATT
  GGAAACCGTACTACAAGCTGACCTGGGAAGAGAAGAAAAAGTTCGACGAGAAACAGAGCCTTCGAGCTTC
  AAGGATCCGAGCCGAGATGTTCGCCAAGGGCCAGCCGGTCGCGCCCTATAACACCACGCAGTTCCTCATG
  GATGATCACGACCAGGAGGAGCCGGACCTCAAAACCGGCCTGTACTCCAAGCGGGCCGCCGCCAAATCCG
  ACGACACCAGCGATGACGACTTCATGGAAGAAGGGGGTGAGGAGGATGGGGGCAGCGATGGGATGGGAGG
  GGACGGCAGCGAGTTTCTGCAGCGGGACTTCTCGGAGACGTACGAGCGGTACCACACGGAGAGCCTGCAG
  AACATGAGCAAGCAGGAGCTCATCAAGGAGTACCTGGAACTGGAGAAGTGCCTCTCGCGCATGGAGGACG
  AGAACAACCGGCTGCGGCTGGAGAGCAAGCGGCTGGGTGGCGACGACGCGCGTGTGCGGGAGCTGGAGCT
  GGAGCTGGACCGGCTGCGCGCCGAGAACCTCCAGCTGCTGACCGAGAACGAACTGCACCGGCAGCAGGAG
  CGAGCGCCGCTTTCCAAGTTTGGAGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200536 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAEPFLSEYQHQPQTSNCTGAAAVQEELNPERPPGAEERVPEEDSRWQSRAFPQLGGRPGPEGEGSLESQ
  PPPLQTQACPESSCLREGEKGQNGDDSSAGGDFPPPAEVEPTPEAELLAQPCHDSEASKLGAPAAGGEEE
  WGQQQRQLGKKKHRRRPSKKKRHWKPYYKLTWEEKKKFDEKQSLRASRIRAEMFAKGQPVAPYNTTQFLM
  DDHDQEEPDLKTGLYSKRAAAKSDDTSDDDFMEEGGEEDGGSDGMGGDGSEFLQRDFSETYERYHTESLQ
  NMSKQELIKEYLELEKCLSRMEDENNRLRLESKRLGGDDARVRELELELDRLRAENLQLLTENELHRQQE
  RAPLSKFGD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006460
• ORF Size: 1077 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006460.3
• RefSeq Size: 4785 bp
• RefSeq ORF: 1080 bp
• Locus ID: 10614
• UniProt ID: O94992
• Cytogenetics: 17q21.31
• Protein Families: Transcription Factors
• MW: 40.6 kDa
• Gene Summary: Expression of this gene is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. This gene has no introns. [provided by RefSeq, Jul 2008]