ZCWCC1 (MORC2) (NM_014941) Human Tagged ORF Clone

CAT#: RC200518

MORC2 (Myc-DDK-tagged)-Human MORC family CW-type zinc finger 2 (MORC2)

Product Images

Circular map for RC200518

• 

  Western blot validation of overexpression lysate (Cat# LY414907) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200518 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZCWCC1
• Synonyms: CMT2Z; DIGFAN; ZCW3; ZCWCC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200518 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTTGCTTTTTGGATGATGGAGCAGGAATGGATCCAAGTGATGCTGCCAGTGTGATCCAGTTTGGGA
  AGTCGGCCAAGCGAACACCTGAGTCTACTCAGATTGGGCAGTACGGGAATGGGTTAAAATCGGGCTCAAT
  GCGCATTGGGAAGGATTTTATCCTGTTCACCAAGAAGGAAGACACCATGACCTGCCTCTTCCTGTCTCGC
  ACGTTTCATGAGGAAGAAGGCATTGATGAAGTGATAGTCCCACTGCCCACCTGGAATGCTCGGACCCGGG
  AACCTGTCACAGACAATGTAGAGAAATTTGCCATTGAGACAGAACTCATCTATAAGTACTCTCCATTCCG
  CACTGAGGAGGAAGTGATGACCCAGTTTATGAAGATTCCTGGGGACAGCGGAACATTGGTGATCATCTTC
  AATCTCAAACTCATGGATAATGGAGAGCCAGAACTAGACATAATCTCAAATCCAAGAGATATCCAGATGG
  CAGAGACGTCCCCAGAGGGCACGAAGCCAGAGCGGCGCTCGTTCCGTGCCTATGCCGCTGTGCTCTATAT
  TGATCCCCGGATGAGGATCTTCATCCATGGGCACAAGGTGCAGACCAAGAGGCTCTCCTGCTGCCTGTAC
  AAGCCCAGGATGTACAAGTACACGTCAAGCCGTTTCAAGACCCGTGCGGAGCAGGAGGTGAAGAAAGCAG
  AGCACGTAGCAAGGATTGCTGAAGAGAAGGCGCGGGAGGCAGAGAGCAAAGCTCGGACATTAGAAGTACG
  CCTAGGTGGAGACCTCACGCGGGACTCCAGGGTGATGTTGCGACAGGTCCAGAACAGAGCCATCACTCTG
  CGCAGAGAAGCCGATGTCAAGAAGAGGATCAAGGAGGCCAAGCAGCGAGCACTTAAAGAACCTAAGGAAC
  TGAATTTTGTTTTTGGTGTCAACATTGAACACCGGGATCTGGATGGCATGTTCATCTACAACTGTAGCCG
  ACTGATCAAAATGTATGAGAAAGTGGGCCCACAGCTGGAAGGGGGCATGGCATGTGGCGGGGTTGTTGGG
  GTTGTTGATGTGCCCTACCTGGTCCTGGAGCCTACACACAACAAACAGGACTTTGCTGATGCCAAGGAGT
  ACCGGCACCTGCTCCGAGCAATGGGGGAGCACCTGGCGCAGTATTGGAAGGATATTGCCATCGCCCAGAG
  GGGAATCATCAAGTTCTGGGATGAGTTTGGCTACCTCTCTGCCAACTGGAACCAGCCCCCATCCAGTGAG
  CTGCGTTACAAACGCCGGAGAGCTATGGAAATCCCCACCACCATCCAGTGCGATTTGTGTCTGAAATGGA
  GAACCCTCCCCTTCCAGCTGAGTTCTGTGGAAAAAGATTACCCTGACACCTGGGTTTGCTCCATGAACCC
  TGATCCTGAACAGGACCGGTGTGAGGCTTCTGAACAAAAGCAGAAGGTTCCCCTGGGAACATTCAGAAAG
  GACATGAAGACGCAGGAAGAGAAGCAGAAACAACTGACAGAGAAAATTCGCCAGCAGCAGGAGAAGCTGG
  AGGCCCTTCAGAAAACCACACCCATCCGCTCCCAAGCAGACCTGAAGAAATTGCCCTTGGAAGTGACCAC
  CAGACCTTCCACTGAGGAACCTGTGCGTAGACCTCAGCGTCCTCGGTCGCCCCCTTTACCTGCTGTGATC
  AGGAACGCCCCCAGCAGACCCCCTTCTTTGCCAACTCCTAGACCAGCCAGCCAGCCCCGAAAGGCTCCTG
  TCATCAGCAGTACCCCAAAGCTCCCTGCTTTGGCAGCCCGGGAGGAGGCCAGCACATCTAGGCTGCTCCA
  GCCACCTGAGGCACCCCGAAAGCCTGCCAACACTCTCGTCAAGACTGCATCCCGACCTGCCCCTCTGGTG
  CAGCAACTGTCACCATCTTTACTGCCCAACTCCAAGAGCCCTCGGGAGGTTCCTTCTCCCAAAGTCATCA
  AGACTCCAGTGGTGAAGAAGACAGAGTCACCCATCAAACTCTCCCCGGCTACCCCTAGTCGGAAGCGGAG
  TGTCGCAGTTTCTGATGAGGAAGAAGTTGAGGAGGAAGCTGAGAGGAGGAAGGAGAGGTGCAAGCGGGGC
  AGATTTGTTGTGAAGGAGGAAAAGAAGGACTCGAATGAGCTCTCAGACAGTGCTGGGGAAGAGGACTCGG
  CTGACCTCAAGAGAGCTCAGAAAGATAAAGGGCTGCACGTGGAGGTGCGTGTGAACAGGGAGTGGTACAC
  GGGCCGTGTCACAGCCGTGGAGGTGGGCAAGCATGTGGTGCGGTGGAAGGTGAAGTTTGACTACGTGCCC
  ACAGACACGACACCAAGAGACCGCTGGGTGGAGAAAGGCAGTGAGGATGTGCGGCTGATGAAACCCCCTT
  CTCCGGAACATCAGAGCCTTGATACACAACAGGAGGGCGGGGAGGAGGAGGTGGGCCCTGTGGCCCAGCA
  GGCCATAGCTGTCGCAGAGCCCTCCACTTCCGAATGCCTCCGCATTGAGCCTGACACCACTGCCCTGAGC
  ACCAATCACGAGACCATCGACCTGCTTGTCCAGATCCTCCGGAATTGTTTACGGTACTTCCTGCCTCCAA
  GTTTCCCCATCTCCAAGAAGCAGCTGAGTGCTATGAATTCAGATGAGCTAATATCTTTTCCTCTGAAGGA
  GTACTTCAAGCAATATGAAGTAGGGCTCCAAAACCTGTGCAATTCCTACCAGAGCCGTGCTGACTCCCGG
  GCCAAGGCCTCCGAGGAAAGCCTGCGCACCTCCGAGAGGAAGCTCCGCGAGACGGAGGAGAAGCTGCAGA
  AGCTGAGGACCAACATCGTGGCACTCCTGCAAAAGGTGCAGGAGGACATAGACATCAACACAGATGATGA
  GCTGGACGCCTACATTGAGGACCTCATCACCAAGGGGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200518 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLCFLDDGAGMDPSDAASVIQFGKSAKRTPESTQIGQYGNGLKSGSMRIGKDFILFTKKEDTMTCLFLSR
  TFHEEEGIDEVIVPLPTWNARTREPVTDNVEKFAIETELIYKYSPFRTEEEVMTQFMKIPGDSGTLVIIF
  NLKLMDNGEPELDIISNPRDIQMAETSPEGTKPERRSFRAYAAVLYIDPRMRIFIHGHKVQTKRLSCCLY
  KPRMYKYTSSRFKTRAEQEVKKAEHVARIAEEKAREAESKARTLEVRLGGDLTRDSRVMLRQVQNRAITL
  RREADVKKRIKEAKQRALKEPKELNFVFGVNIEHRDLDGMFIYNCSRLIKMYEKVGPQLEGGMACGGVVG
  VVDVPYLVLEPTHNKQDFADAKEYRHLLRAMGEHLAQYWKDIAIAQRGIIKFWDEFGYLSANWNQPPSSE
  LRYKRRRAMEIPTTIQCDLCLKWRTLPFQLSSVEKDYPDTWVCSMNPDPEQDRCEASEQKQKVPLGTFRK
  DMKTQEEKQKQLTEKIRQQQEKLEALQKTTPIRSQADLKKLPLEVTTRPSTEEPVRRPQRPRSPPLPAVI
  RNAPSRPPSLPTPRPASQPRKAPVISSTPKLPALAAREEASTSRLLQPPEAPRKPANTLVKTASRPAPLV
  QQLSPSLLPNSKSPREVPSPKVIKTPVVKKTESPIKLSPATPSRKRSVAVSDEEEVEEEAERRKERCKRG
  RFVVKEEKKDSNELSDSAGEEDSADLKRAQKDKGLHVEVRVNREWYTGRVTAVEVGKHVVRWKVKFDYVP
  TDTTPRDRWVEKGSEDVRLMKPPSPEHQSLDTQQEGGEEEVGPVAQQAIAVAEPSTSECLRIEPDTTALS
  TNHETIDLLVQILRNCLRYFLPPSFPISKKQLSAMNSDELISFPLKEYFKQYEVGLQNLCNSYQSRADSR
  AKASEESLRTSERKLRETEEKLQKLRTNIVALLQKVQEDIDINTDDELDAYIEDLITKGD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014941
• ORF Size: 2910 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014941.3
• RefSeq Size: 4467 bp
• RefSeq ORF: 2913 bp
• Locus ID: 22880
• UniProt ID: Q9Y6X9
• Cytogenetics: 22q12.2
• MW: 110.7 kDa
• Gene Summary: This gene encodes a member of the Microrchidia (MORC) protein superfamily. The encoded protein is known to regulate the condensation of heterochromatin in response to DNA damage and play a role in repressing transcription. The protein has been found to regulate the activity of ATP citrate lyase via specific interaction with this enzyme in the cytosol of lipogenic breast cancer cells. The protein also plays a role in lipogenesis and adipocyte differentiation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2016]