PCOLCE (NM_002593) Human Tagged ORF Clone

CAT#: RC200515

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PCOLCE (Myc-DDK-tagged)-Human procollagen C-endopeptidase enhancer (PCOLCE)



  "NM_002593" in other vectors (7)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 118.00

USD 616.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol PCOLCE
Synonyms PCPE; PCPE-1; PCPE1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC200515 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGCCTGCAGCCACAGCCTCCCTCCTGGGGCCCCTCCTCACTGCCTGCGCCCTGCTGCCTTTTGCCC
AGGGCCAGACCCCCAACTACACCAGACCCGTGTTCCTGTGCGGAGGGGATGTGAAGGGGGAATCAGGTTA
CGTGGCAAGTGAGGGGTTCCCCAACCTCTACCCCCCTAATAAGGAGTGCATCTGGACCATAACGGTCCCC
GAGGGCCAGACTGTGTCCCTCTCATTCCGAGTCTTCGACCTGGAGCTGCACCCCGCCTGCCGCTACGATG
CTCTGGAGGTCTTCGCTGGGTCTGGGACTTCCGGCCAGCGGCTCGGACGCTTTTGTGGGACCTTCCGGCC
TGCGCCCCTAGTCGCCCCCGGCAACCAGGTGACCCTGAGGATGACGACGGATGAGGGCACAGGAGGACGA
GGCTTCCTGCTCTGGTACAGCGGGCGGGCCACCTCGGGCACTGAGCACCAATTTTGCGGGGGGCGGCTGG
AGAAGGCCCAGGGAACCCTGACCACGCCCAACTGGCCCGAGTCCGATTACCCCCCGGGCATCAGCTGTTC
CTGGCACATCATCGCGCCCCCGGACCAGGTCATCGCGCTGACCTTCGAGAAGTTTGACCTGGAGCCGGAC
ACCTACTGCCGCTATGACTCGGTCAGCGTGTTCAACGGAGCCGTGAGCGACGACTCCCGGAGGCTGGGGA
AGTTCTGCGGCGACGCAGTCCCGGGCTCCATCTCCTCCGAAGGGAATGAACTCCTCGTCCAGTTCGTCTC
AGATCTCAGTGTCACCGCTGATGGCTTCTCAGCCTCCTACAAGACCCTGCCGCGGGGCACTGCCAAAGAA
GGGCAAGGGCCCGGCCCCAAACGGGGAACTGAGCCTAAAGTCAAGCTGCCCCCCAAGTCCCAACCTCCGG
AGAAAACAGAGGAATCTCCTTCAGCCCCTGATGCACCCACCTGCCCAAAGCAGTGCCGCCGGACAGGCAC
CTTGCAGAGCAACTTCTGTGCCAGCAGCCTTGTGGTGACTGCGACAGTGAAGTCCATGGTTCGGGAGCCA
GGGGAGGGCCTTGCCGTGACTGTCAGTCTTATTGGTGCTTATAAAACTGGAGGACTGGACCTGCCTTCTC
CACCCACTGGTGCCTCCCTGAAGTTTTACGTGCCTTGCAAGCAGTGCCCCCCCATGAAGAAAGGAGTCAG
TTATCTGCTGATGGGCCAGGTAGAAGAGAACAGAGGCCCCGTCCTTCCTCCAGAGAGCTTTGTGGTTCTC
CACCGGCCCAACCAGGACCAGATCCTCACCAACCTAAGCAAGAGGAAGTGCCCCTCTCAACCTGTGCGGG
CTGCTGCGTCCCAGGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC200515 protein sequence
Red=Cloning site Green=Tags(s)

MLPAATASLLGPLLTACALLPFAQGQTPNYTRPVFLCGGDVKGESGYVASEGFPNLYPPNKECIWTITVP
EGQTVSLSFRVFDLELHPACRYDALEVFAGSGTSGQRLGRFCGTFRPAPLVAPGNQVTLRMTTDEGTGGR
GFLLWYSGRATSGTEHQFCGGRLEKAQGTLTTPNWPESDYPPGISCSWHIIAPPDQVIALTFEKFDLEPD
TYCRYDSVSVFNGAVSDDSRRLGKFCGDAVPGSISSEGNELLVQFVSDLSVTADGFSASYKTLPRGTAKE
GQGPGPKRGTEPKVKLPPKSQPPEKTEESPSAPDAPTCPKQCRRTGTLQSNFCASSLVVTATVKSMVREP
GEGLAVTVSLIGAYKTGGLDLPSPPTGASLKFYVPCKQCPPMKKGVSYLLMGQVEENRGPVLPPESFVVL
HRPNQDQILTNLSKRKCPSQPVRAAASQD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_002593
ORF Size 1347 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002593.4
RefSeq Size 1651 bp
RefSeq ORF 1350 bp
Locus ID 5118
UniProt ID Q15113
Cytogenetics 7q22.1
Domains CUB, NTR
Protein Families Secreted Protein
MW 48 kDa
Gene Summary Fibrillar collagen types I-III are synthesized as precursor molecules known as procollagens. These precursors contain amino- and carboxyl-terminal peptide extensions known as N- and C-propeptides, respectively, which are cleaved, upon secretion of procollagen from the cell, to yield the mature triple helical, highly structured fibrils. This gene encodes a glycoprotein which binds and drives the enzymatic cleavage of type I procollagen and heightens C-proteinase activity. [provided by RefSeq, Jul 2008]

Other Versions

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