AKAP1 (NM_003488) Human Tagged ORF Clone

CAT#: RC200506

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  • TrueORF®

AKAP1 (Myc-DDK-tagged)-Human A kinase (PRKA) anchor protein 1 (AKAP1), nuclear gene encoding mitochondrial protein



  "NM_003488" in other vectors (6)

USD 98.00

USD 560.00


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Size
    • 10 ug


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Specifications

Product Data
Product Name AKAP1 (NM_003488) Human Tagged ORF Clone
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol AKAP1
Synonyms AKAP; AKAP84; AKAP121; AKAP149; D-AKAP1; PPP1R43; PRKA1; SAKAP84; TDRD17
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>RC200506 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAATCCAGTTCCGTTCGCTCTTCCCCTTGGCATTGCCTGGGATGCTGGCGCTCCTCGGCTGGTGGT
GGTTTTTCTCTCGTAAAAAAGGCCATGTCAGCAGCCATGATGAGCAGCAGGTGGAGGCTGGTGCTGTGCA
GCTGAGGGCTGACCCTGCCATCAAGGAACCTCTCCCCGTGGAAGACGTCTGTCCCAAAGTAGTGTCCACA
CCCCCCAGTGTCACAGAGCCTCCAGAAAAGGAACTGTCCACCGTGAGCAAGCTGCCTGCAGAGCCCCCAG
CATTGCTCCAGACACACCCACCTTGCCGAAGATCAGAGTCCTCGGGCATTCTTCCTAACACCACAGACAT
GAGATTGCGACCAGGAACACGCAGAGATGACAGTACAAAGCTGGAGCTAGCCCTGACAGGTGGTGAAGCC
AAATCGATTCCTCTAGAGTGCCCCCTTTCATCCCCAAAGGGTGTACTATTCTCCAGCAAATCAGCTGAGG
TGTGTAAGCAAGATTCCCCCTTCAGCAGGGTGCCAAGGAAGGTCCAGCCAGGCTACCCCGTAGTCCCCGC
AGAGAAGCGTAGCTCTGGGGAGAGGGCAAGAGAGACAGGTGGGGCCGAAGGGACTGGTGATGCCGTGTTG
GGGGAAAAGGTGCTTGAAGAAGCTCTGTTGTCTCGGGAGCATGTCTTGGAATTGGAGAACAGCAAGGGCC
CCAGCCTGGCCTCTTTAGAGGGGGAAGAAGATAAGGGGAAGAGCAGCTCATCCCAGGTGGTGGGGCCAGT
GCAGGAGGAAGAGTATGTAGCAGAGAAGTTGCCAAGTAGGTTCATCGAGTCGGCTCACACAGAGCTGGCA
AAGGACGATGCGGCGCCAGCACCCCCAGTCGCAGACGCCAAAGCCCAGGATAGAGGTGTCGAGGGAGAAC
TGGGCAATGAGGAGAGCTTGGATAGAAATGAGGAGGGCTTGGATAGAAATGAGGAGGGCTTGGATAGAAA
TGAGGAGAGCTTGGATAGAAATGAGGAGGGCTTGGATAGAAATGAGGAGATTAAGCGGGCTGCCTTCCAG
ATAATCTCCCAAGTGATCTCAGAAGCAACCGAACAGGTGCTGGCCACCACGGTTGGCAAGGTTGCAGGTC
GTGTGTGTCAGGCCAGTCAGCTCCAAGGGCAGAAGGAAGAGAGCTGTGTCCCAGTTCACCAGAAAACTGT
CTTGGGCCCAGACACTGCGGAGCCTGCCACAGCAGAGGCAGCTGTTGCCCCGCCGGATGCTGGCCTCCCC
TTGCCAGGCCTACCAGCAGAGGGCTCACCACCACCAAAGACCTACGTGAGCTGCCTGAAGAGCCTTCTGT
CCAGCCCCACCAAGGACAGTAAGCCAAATATCTCTGCACACCACATCTCCCTGGCCTCCTGCCTGGCACT
GACCACCCCCAGTGAAGAGTTGCCGGACCGGGCAGGCATCCTGGTGGAAGATGCCACCTGTGTCACCTGC
ATGTCAGACAGCAGCCAAAGTGTCCCTTTGGTGGCTTCTCCAGGACACTGCTCAGATTCTTTCAGCACTT
CAGGGCTTGAAGACTCTTGCACAGAGACCAGCTCGAGCCCCAGGGACAAGGCCATCACCCCGCCACTGCC
AGAAAGTACTGTGCCCTTCAGCAATGGGGTGCTGAAGGGGGAGTTGTCAGACTTGGGGGCTGAGGATGGA
TGGACCATGGATGCGGAAGCAGATCATTCAGGAGGTTCTGACAGGAACAGCATGGATTCCGTGGATAGCT
GTTGCAGTCTCAAGAAGACTGAGAGCTTCCAAAATGCCCAGGCAGGCTCCAACCCTAAGAAGGTCGACCT
CATCATCTGGGAGATCGAGGTGCCAAAGCACTTAGTCGGTCGGCTAATTGGCAAGCAGGGGCGCTATGTG
AGTTTTCTGAAGCAAACATCTGGTGCCAAGATCTACATTTCAACCCTGCCTTACACCCAGAGCGTCCAGA
TCTGCCACATAGAAGGCTCTCAACATCATGTAGACAAAGCGCTGAACTTGATTGGGAAGAAGTTCAAAGA
GCTGAACCTCACCAATATCTACGCTCCCCCATTGCCTTCACTGGCACTGCCTTCTCTGCCGATGACATCC
TGGCTCATGCTGCCTGATGGCATCACCGTGGAGGTCATTGTGGTCAACCAGGTCAATGCCGGGCACCTGT
TCGTGCAGCAGCACACACACCCTACCTTCCACGCGCTGCGCAGCCTCGACCAGCAGATGTACCTCTGTTA
CTCTCAGCCTGGAATCCCCACCTTGCCCACCCCAGTGGAAATAACGGTCATCTGTGCCGCCCCTGGTGCG
GACGGGGCCTGGTGGCGAGCCCAAGTGGTTGCCTCCTACGAGGAGACCAACGAAGTGGAGATTCGATACG
TGCACTACGGCGGATATAAGAGGGTGAAAGTAGACGTGCTCCGGCAAATCAGGTCTGACTTTGTCACCCT
GCCGTTTCAGGGAGCAGAAGTCCTTCTGGACAGTGTGATGCCCCTGTCAGACGATGACCAGTTTTCACCG
GAAGCAGATGCCGCCATGAGCGAGATGACGGGGAATACAGCACTGCTTGCTCAGGTGACAAGTTACAGTC
CAACTGGTCTTCCTCTGATTCAGCTGTGGAGTGTGGTTGGAGATGAAGTGGTGTTGATAAACCGGTCCCT
GGTGGAGCGAGGCCTTGCCCAGTGGGTAGACAGCTACTACACAAGCCTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC200506 protein sequence
Red=Cloning site Green=Tags(s)

MAIQFRSLFPLALPGMLALLGWWWFFSRKKGHVSSHDEQQVEAGAVQLRADPAIKEPLPVEDVCPKVVST
PPSVTEPPEKELSTVSKLPAEPPALLQTHPPCRRSESSGILPNTTDMRLRPGTRRDDSTKLELALTGGEA
KSIPLECPLSSPKGVLFSSKSAEVCKQDSPFSRVPRKVQPGYPVVPAEKRSSGERARETGGAEGTGDAVL
GEKVLEEALLSREHVLELENSKGPSLASLEGEEDKGKSSSSQVVGPVQEEEYVAEKLPSRFIESAHTELA
KDDAAPAPPVADAKAQDRGVEGELGNEESLDRNEEGLDRNEEGLDRNEESLDRNEEGLDRNEEIKRAAFQ
IISQVISEATEQVLATTVGKVAGRVCQASQLQGQKEESCVPVHQKTVLGPDTAEPATAEAAVAPPDAGLP
LPGLPAEGSPPPKTYVSCLKSLLSSPTKDSKPNISAHHISLASCLALTTPSEELPDRAGILVEDATCVTC
MSDSSQSVPLVASPGHCSDSFSTSGLEDSCTETSSSPRDKAITPPLPESTVPFSNGVLKGELSDLGAEDG
WTMDAEADHSGGSDRNSMDSVDSCCSLKKTESFQNAQAGSNPKKVDLIIWEIEVPKHLVGRLIGKQGRYV
SFLKQTSGAKIYISTLPYTQSVQICHIEGSQHHVDKALNLIGKKFKELNLTNIYAPPLPSLALPSLPMTS
WLMLPDGITVEVIVVNQVNAGHLFVQQHTHPTFHALRSLDQQMYLCYSQPGIPTLPTPVEITVICAAPGA
DGAWWRAQVVASYEETNEVEIRYVHYGGYKRVKVDVLRQIRSDFVTLPFQGAEVLLDSVMPLSDDDQFSP
EADAAMSEMTGNTALLAQVTSYSPTGLPLIQLWSVVGDEVVLINRSLVERGLAQWVDSYYTSL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_003488
ORF Size 2709 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003488.1, NM_003488.2, NM_003488.3, NP_003479.1
RefSeq Size 3968 bp
RefSeq ORF 2712 bp
Locus ID 8165
Cytogenetics 17q22
Domains TUDOR, KH, TUDOR
Protein Families Druggable Genome, Transmembrane
MW 97.4 kDa
Gene Summary The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein binds to type I and type II regulatory subunits of PKA and anchors them to the mitochondrion. This protein is speculated to be involved in the cAMP-dependent signal transduction pathway and in directing RNA to a specific cellular compartment. [provided by RefSeq, Jul 2008]

Citations (1)

Other Versions

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