CDC25A (NM_001789) Human Tagged ORF Clone
CDC25A (Myc-DDK-tagged)-Human cell division cycle 25 homolog A (S. pombe) (CDC25A), transcript variant 1
|Product Name||CDC25A (NM_001789) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC200496 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC200496 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||1572 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at firstname.lastname@example.org or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq||NM_001789.1, NM_001789.2, NP_001780.2|
|RefSeq Size||3717 bp|
|RefSeq ORF||1575 bp|
|Protein Families||Druggable Genome, Phosphatase|
|Protein Pathways||Cell cycle, Progesterone-mediated oocyte maturation|
|Gene Summary||CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]|
|The use of this cDNA Clones has been cited in the following citations:|
Human TRIB2 Oscillates during the Cell Cycle and Promotes Ubiquitination and Degradation of CDC25C
,Liang, KL;Paredes, R;Carmody, R;Eyers, PA;Meyer, S;McCarthy, TV;Keeshan, K;,
Int J Mol Sci 2016
,PubMed ID 27563873
miRNA-497 Negatively Regulates the Growth and Motility of Chondrosarcoma Cells by Targeting Cdc25A
,Lu, Y;Li, F;Xu, T;Sun, J;,
Oncology Research Mar 2016
,PubMed ID 27053344
|cDNA Clone Resources|
|SC127411||CDC25A (untagged)-Human cell division cycle 25 homolog A (S. pombe) (CDC25A), transcript variant 1||
|RG200496||CDC25A (GFP-tagged) - Human cell division cycle 25 homolog A (S. pombe) (CDC25A), transcript variant 1||
|RC200496L1||Lenti ORF clone of Human cell division cycle 25 homolog A (S. pombe) (CDC25A), transcript variant 1, Myc-DDK-tagged||
|RC200496L2||Lenti ORF clone of Human cell division cycle 25 homolog A (S. pombe) (CDC25A), transcript variant 1, mGFP tagged||
|RC200496L3||Lenti ORF clone of Human cell division cycle 25 homolog A (S. pombe) (CDC25A), transcript variant 1, Myc-DDK-tagged||
|RC200496L4||Lenti ORF clone of Human cell division cycle 25 homolog A (S. pombe) (CDC25A), transcript variant 1, mGFP tagged||