CDK1 (NM_001786) Human Tagged ORF Clone
CDK1 (Myc-DDK-tagged)-Human cyclin-dependent kinase 1 (CDK1), transcript variant 1
|Product Name||CDK1 (NM_001786) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||CDC2; CDC28A; P34CDC2|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC200495 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC200495 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||891 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||1923 bp|
|RefSeq ORF||894 bp|
|Domains||pkinase, TyrKc, S_TKc|
|Protein Families||Druggable Genome, Protein Kinase, Stem cell - Pluripotency|
|Protein Pathways||Cell cycle, Gap junction, Oocyte meiosis, p53 signaling pathway, Progesterone-mediated oocyte maturation|
|Gene Summary||The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]|
|The use of this cDNA Clones has been cited in the following citations:|
The Photorhabdus Virulence Cassettes RRSP-Like Effector Interacts With Cyclin-Dependent Kinase 1 and Causes Mitotic Defects in Mammalian Cells
,Wang, X;Shen, J;Jiang, F;Jin, Q;,
Front Microbiol 2020
,PubMed ID 32231646
Potent Activity of Composite Cyclin Dependent Kinase Inhibition against Hepatocellular Carcinoma
,Shao, YY;Li, YS;Hsu, HW;Lin, H;Wang, HY;Wo, RR;Cheng, AL;Hsu, CH;,
Cancers (Basel) 2019
,PubMed ID 31561409
The Role of p27Kip1 in Dasatinib-Enhanced Paclitaxel Cytotoxicity in Human Ovarian Cancer Cells
,Xiao-Feng Le, Weiqun Mao, Guangan He, Francois-Xavier Claret, Weiya Xia, Ahmed Ashour Ahmed, Mien-Chie Hung, Zahid H. Siddik, and Robert C. Bast, Jr,
J Natl Cancer Inst, Aug 2011; 10.1093/jnci/djr280.
|cDNA Clone Resources|
|SC111605||CDK1 (untagged)-Human cyclin-dependent kinase 1 (CDK1), transcript variant 1||
|SC323387||CDK1 (untagged)-Kinase deficient mutant (K33M) of Human cell division cycle 2, G1 to S and G2 to M (CDC2), transcript variant 1||
|RG200495||CDK1 (GFP-tagged) - Human cyclin-dependent kinase 1 (CDK1), transcript variant 1||
|RC200495L1||Lenti ORF clone of Human cyclin-dependent kinase 1 (CDK1), transcript variant 1, Myc-DDK-tagged||
|RC200495L2||Lenti ORF clone of Human cyclin-dependent kinase 1 (CDK1), transcript variant 1, mGFP tagged||
|RC200495L3||Lenti ORF clone of Human cyclin-dependent kinase 1 (CDK1), transcript variant 1, Myc-DDK-tagged||
|RC200495L4||Lenti ORF clone of Human cyclin-dependent kinase 1 (CDK1), transcript variant 1, mGFP tagged||