CPT2 (NM_000098) Human Tagged ORF Clone

CAT#: RC200484

CPT2 (Myc-DDK-tagged)-Human carnitine palmitoyltransferase 2 (CPT2), nuclear gene encoding mitochondrial protein

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro


  "NM_000098" in other vectors (4)

Reconstitution Protocol

USD 612.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (5)
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol CPT2
Synonyms CPT1; CPTASE; IIAE4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC200484 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGCCCCGCCTGCTGCTGCGCGCCTGGCCCCGGGGCCCCGCGGTTGGTCCGGGAGCCCCCAGTCGGC
CCCTCAGCGCCGGCTCCGGGCCCGGCCAGTACCTGCAGCGCAGCATCGTGCCCACCATGCACTACCAGGA
CAGCCTGCCCAGGCTGCCTATTCCCAAACTTGAAGACACCATTAGGAGATACCTCAGTGCACAGAAGCCT
CTCTTGAATGATGGCCAGTTCAGGAAAACAGAACAATTTTGCAAGAGTTTTGAAAATGGGATTGGAAAAG
AACTGCATGAGCAGCTGGTTGCTCTGGACAAACAGAATAAACATACAAGCTACATTTTGGGACCCTGGTT
TGATATGTACCTATCTGCTCGAGACTCCGTTGTTCTGAACTTTAATCCATTTATGGCTTTCAATCCTGAC
CCAAAATCTGAGTATAATGACCAGCTCACCCGGGCAACCAACATGACTGTTTCTGCCATCCGGTTTCTGA
AGACACTCCGGGCTGGCCTTCTGGAGCCAGAAGTGTTCCACTTGAACCCTGCAAAAAGTGACACTATCAC
CTTCAAGAGACTCATACGCTTTGTGCCTTCCTCTCTGTCCTGGTATGGGGCCTACCTGGTCAATGCGTAT
CCCCTGGATATGTCCCAGTATTTTCGGCTTTTCAACTCAACTCGTTTACCCAAACCCAGTCGGGATGAAC
TCTTCACTGATGACAAGGCCAGACACCTCCTGGTCCTAAGGAAAGGAAATTTTTATATCTTTGATGTCCT
GGATCAAGATGGGAACATTGTGAGCCCCTCGGAAATCCAGGCACATCTGAAGTACATTCTCTCAGACAGC
AGCCCCGCCCCCGAGTTTCCCCTGGCATACCTGACCAGTGAGAACCGAGACATCTGGGCAGAGCTCAGGC
AGAAGCTGATGAGTAGTGGCAATGAGGAGAGCCTGAGGAAAGTGGACTCGGCAGTGTTCTGTCTCTGCCT
AGATGACTTCCCCATTAAGGACCTTGTCCACTTGTCCCACAATATGCTGCATGGGGATGGCACAAACCGC
TGGTTTGATAAATCCTTTAACCTCATTATCGCCAAGGATGGCTCTACTGCCATCCACTTTGAGCACTCTT
GGGGTGATGGTGTGGCAGTGCTCAGATTTTTTAATGAAGTATTTAAAGACAGCACTCAGACCCCTGCCGT
CACTCCACAGAGCCAGCCAGCTACCACTGACTCTACTGTCACGGTGCAGAAACTCAACTTCGAGCTGACT
GATGCCTTAAAGACTGGCATCACAGCTGCTAAGGAAAAGTTTGATGCCACCATGAAAACCCTCACTATTG
ACTGCGTCCAGTTTCAGAGAGGAGGCAAAGAATTCCTGAAGAAGCAAAAGCTGAGCCCTGACGCAGTTGC
CCAGCTGGCATTCCAGATGGCCTTCCTGCGGCAGTACGGGCAGACAGTGGCCACCTACGAGTCCTGTAGC
ACTGCCGCATTCAAGCACGGCCGCACTGAGACCATCCGCCCGGCCTCCGTCTATACAAAGAGGTGCTCTG
AGGCCTTTGTCAGGGAGCCCTCCAGGCACAGTGCTGGTGAGCTTCAGCAGATGATGGTTGAGTGCTCCAA
GTACCATGGCCAGCTGACCAAAGAAGCAGCAATGGGCCAGGGCTTTGACCGACACTTGTTTGCTCTGCGG
CATCTGGCAGCAGCCAAAGGGATCATCTTGCCTGAGCTCTACCTGGACCCTGCATACGGGCAGATAAACC
ACAATGTCCTGTCCACGAGCACACTGAGCAGCCCAGCAGTGAACCTTGGGGGCTTTGCCCCTGTGGTCTC
TGATGGCTTTGGTGTTGGGTATGCTGTTCATGACAACTGGATAGGCTGCAATGTCTCTTCCTACCCAGGC
CGCAATGCCCGGGAGTTTCTCCAATGTGTGGAGAAGGCCTTAGAAGACGTGTTTGATGCCTTAGAAGGCA
AATCCATCAAAAGT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC200484 protein sequence
Red=Cloning site Green=Tags(s)

MVPRLLLRAWPRGPAVGPGAPSRPLSAGSGPGQYLQRSIVPTMHYQDSLPRLPIPKLEDTIRRYLSAQKP
LLNDGQFRKTEQFCKSFENGIGKELHEQLVALDKQNKHTSYILGPWFDMYLSARDSVVLNFNPFMAFNPD
PKSEYNDQLTRATNMTVSAIRFLKTLRAGLLEPEVFHLNPAKSDTITFKRLIRFVPSSLSWYGAYLVNAY
PLDMSQYFRLFNSTRLPKPSRDELFTDDKARHLLVLRKGNFYIFDVLDQDGNIVSPSEIQAHLKYILSDS
SPAPEFPLAYLTSENRDIWAELRQKLMSSGNEESLRKVDSAVFCLCLDDFPIKDLVHLSHNMLHGDGTNR
WFDKSFNLIIAKDGSTAIHFEHSWGDGVAVLRFFNEVFKDSTQTPAVTPQSQPATTDSTVTVQKLNFELT
DALKTGITAAKEKFDATMKTLTIDCVQFQRGGKEFLKKQKLSPDAVAQLAFQMAFLRQYGQTVATYESCS
TAAFKHGRTETIRPASVYTKRCSEAFVREPSRHSAGELQQMMVECSKYHGQLTKEAAMGQGFDRHLFALR
HLAAAKGIILPELYLDPAYGQINHNVLSTSTLSSPAVNLGGFAPVVSDGFGVGYAVHDNWIGCNVSSYPG
RNAREFLQCVEKALEDVFDALEGKSIKS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_000098
ORF Size 1974 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000098.3
RefSeq Size 3110 bp
RefSeq ORF 1977 bp
Locus ID 1376
UniProt ID P23786
Cytogenetics 1p32.3
Domains Carn_acyltransf
Protein Families Druggable Genome
Protein Pathways Fatty acid metabolism, PPAR signaling pathway
MW 73.8 kDa
Gene Summary The protein encoded by this gene is a nuclear protein which is transported to the mitochondrial inner membrane. Together with carnitine palmitoyltransferase I, the encoded protein oxidizes long-chain fatty acids in the mitochondria. Defects in this gene are associated with mitochondrial long-chain fatty-acid (LCFA) oxidation disorders. [provided by RefSeq, Jul 2008]

Other Versions

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