FOXO1 (NM_002015) Human Tagged ORF Clone

USD 98.00

USD 560.00


Availability*
2 Weeks

Size
    • 10 ug


Product images

Specifications

Product Data
Product Name FOXO1 (NM_002015) Human Tagged ORF Clone
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol FOXO1
Synonyms FKH1; FKHR; FOXO1A
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>RC200477 representing NM_002015
Red=Cloning site Blue=ORF Green=Tags(s)

CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGAGGCGCCTCAGGTGGTGGAGATCGACCCGGACTTCGAGCCGCTGCCCCGGCCGCGCTCGTGCA
CCTGGCCGCTGCCCAGGCCGGAGTTTAGCCAGTCCAACTCGGCCACCTCCAGCCCGGCGCCGTCGGGCAG
CGCGGCTGCCAACCCCGACGCCGCGGCGGGCCTGCCCTCGGCCTCGGCTGCCGCTGTCAGCGCCGACTTC
ATGAGCAACCTGAGCTTGCTGGAGGAGAGCGAGGACTTCCCGCAGGCGCCCGGCTCCGTGGCGGCGGCGG
TGGCGGCGGCGGCCGCCGCGGCCGCCACCGGGGGGCTGTGCGGGGACTTCCAGGGCCCGGAGGCGGGCTG
CCTGCACCCAGCGCCACCGCAGCCCCCGCCGCCCGGGCCGCTGTCGCAGCACCCGCCGGTGCCCCCCGCC
GCCGCTGGGCCGCTCGCGGGGCAGCCGCGCAAGAGCAGCTCGTCCCGCCGCAACGCGTGGGGCAACCTGT
CCTACGCCGACCTCATCACCAAGGCCATCGAGAGCTCGGCGGAGAAGCGGCTCACGCTGTCGCAGATCTA
CGAGTGGATGGTCAAGAGCGTGCCCTACTTCAAGGATAAGGGTGACAGCAACAGCTCGGCGGGCTGGAAG
AATTCAATTCGTCATAATCTGTCCCTACACAGCAAGTTCATTCGTGTGCAGAATGAAGGAACTGGAAAAA
GTTCTTGGTGGATGCTCAATCCAGAGGGTGGCAAGAGCGGGAAATCTCCTAGGAGAAGAGCTGCATCCAT
GGACAACAACAGTAAATTTGCTAAGAGCCGAAGCCGAGCTGCCAAGAAGAAAGCATCTCTCCAGTCTGGC
CAGGAGGGTGCTGGGGACAGCCCTGGATCACAGTTTTCCAAATGGCCTGCAAGCCCTGGCTCTCACAGCA
ATGATGACTTTGATAACTGGAGTACATTTCGCCCTCGAACTAGCTCAAATGCTAGTACTATTAGTGGGAG
ACTCTCACCCATTATGACCGAACAGGATGATCTTGGAGAAGGGGATGTGCATTCTATGGTGTACCCGCCA
TCTGCCGCAAAGATGGCCTCTACTTTACCCAGTCTGTCTGAGATAAGCAATCCCGAAAACATGGAAAATC
TTTTGGATAATCTCAACCTTCTCTCATCACCAACATCATTAACTGTTTCGACCCAGTCCTCACCTGGCAC
CATGATGCAGCAGACGCCGTGCTACTCGTTTGCGCCACCAAACACCAGTTTGAATTCACCCAGCCCAAAC
TACCAAAAATATACATATGGCCAATCCAGCATGAGCCCTTTGCCCCAGATGCCTATACAAACACTTCAGG
ACAATAAGTCGAGTTATGGAGGTATGAGTCAGTATAACTGTGCGCCTGGACTCTTGAAGGAGTTGCTGAC
TTCTGACTCTCCTCCCCATAATGACATTATGACACCAGTTGATCCTGGGGTAGCCCAGCCCAACAGCCGG
GTTCTGGGCCAGAACGTCATGATGGGCCCTAATTCGGTCATGTCAACCTATGGCAGCCAGGTATCTCATA
ACAAAATGATGAATCCCAGCTCCCATACCCACCCTGGACATGCTCAGCAGACATCTGCAGTTAACGGGCG
TCCCCTGCCCCACACGGTAAGCACCATGCCCCACACCTCGGGTATGAACCGCCTGACCCAAGTGAAGACA
CCTGTACAAGTGCCTCTGCCCCACCCCATGCAGATGAGTGCCCTGGGGGGCTACTCCTCCGTGAGCAGCT
GCAATGGCTATGGCAGAATGGGCCTTCTCCACCAGGAGAAGCTCCCAAGTGACTTGGATGGCATGTTCAT
TGAGCGCTTAGACTGTGACATGGAATCCATCATTCGGAATGACCTCATGGATGGAGATACATTGGATTTT
AACTTTGACAATGTGTTGCCCAACCAAAGCTTCCCACACAGTGTCAAGACAACGACACATAGCTGGGTGT
CAGGC


CTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAGGATGACGACG
ATAA
GGTTTAA
>RC200477 representing NM_002015
Red=Cloning site Green=Tags(s)

MAEAPQVVEIDPDFEPLPRPRSCTWPLPRPEFSQSNSATSSPAPSGSAAANPDAAAGLPSASAAAVSADF
MSNLSLLEESEDFPQAPGSVAAAVAAAAAAAATGGLCGDFQGPEAGCLHPAPPQPPPPGPLSQHPPVPPA
AAGPLAGQPRKSSSSRRNAWGNLSYADLITKAIESSAEKRLTLSQIYEWMVKSVPYFKDKGDSNSSAGWK
NSIRHNLSLHSKFIRVQNEGTGKSSWWMLNPEGGKSGKSPRRRAASMDNNSKFAKSRSRAAKKKASLQSG
QEGAGDSPGSQFSKWPASPGSHSNDDFDNWSTFRPRTSSNASTISGRLSPIMTEQDDLGEGDVHSMVYPP
SAAKMASTLPSLSEISNPENMENLLDNLNLLSSPTSLTVSTQSSPGTMMQQTPCYSFAPPNTSLNSPSPN
YQKYTYGQSSMSPLPQMPIQTLQDNKSSYGGMSQYNCAPGLLKELLTSDSPPHNDIMTPVDPGVAQPNSR
VLGQNVMMGPNSVMSTYGSQVSHNKMMNPSSHTHPGHAQQTSAVNGRPLPHTVSTMPHTSGMNRLTQVKT
PVQVPLPHPMQMSALGGYSSVSSCNGYGRMGLLHQEKLPSDLDGMFIERLDCDMESIIRNDLMDGDTLDF
NFDNVLPNQSFPHSVKTTTHSWVSG

LEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-XhoI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_002015
ORF Size 1965 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002015.1, NM_002015.2, NM_002015.3, NM_002015.4, NP_002006.2
RefSeq Size 5723 bp
RefSeq ORF 1968 bp
Locus ID 2308
Cytogenetics 13q14.11
Domains FH
Protein Families Druggable Genome, Transcription Factors
Protein Pathways Insulin signaling pathway, Pathways in cancer, Prostate cancer
MW 69.5 kDa
Gene Summary This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008]

Citations (6)

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