GRB2 (NM_002086) Human Tagged ORF Clone
GRB2 (Myc-DDK-tagged)-Human growth factor receptor-bound protein 2 (GRB2), transcript variant 1
|Product Name||GRB2 (NM_002086) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||ASH; EGFRBP-GRB2; Grb3-3; MST084; MSTP084; NCKAP2|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC200469 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC200469 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||651 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||3303 bp|
|RefSeq ORF||654 bp|
|Protein Families||Druggable Genome|
|Protein Pathways||Acute myeloid leukemia, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Dorso-ventral axis formation, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, Insulin signaling pathway, Jak-STAT signaling pathway, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pathways in cancer, Prostate cancer, Renal cell carcinoma, T cell receptor signaling pathway|
|Gene Summary||The protein encoded by this gene binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]|
|The use of this cDNA Clones has been cited in the following citations:|
Elucidation of protein interactions necessary for the maintenance of the BCR-ABL signaling complex
,Gregor, T;Bosakova, MK;Nita, A;Abraham, SP;Fafilek, B;Cernohorsky, NH;Rynes, J;Foldynova-Trantirkova, S;Zackova, D;Mayer, J;Trantirek, L;Krejci, P;,
Cell. Mol. Life Sci. 2019
,PubMed ID 31820037
Elucidation of protein-protein interactions necessary for maintenance of the BCR-ABL signaling complex
,Gregor, T;Bosakova, MK;Nita, A;Abraham, SP;Fafilek, B;,
Phosphatidic Acid Is a Leukocyte Chemoattractant That Acts through S6 Kinase Signaling
,Kathleen Frondorf, Karen M. Henkels, Michael A. Frohman, and Julian Gomez-Cambronero,
J. Biol. Chem., May 2010; 285: 15837 - 15847
|cDNA Clone Resources|
|SC319370||GRB2 (untagged)-Human growth factor receptor-bound protein 2 (GRB2), transcript variant 1||
|RG200469||GRB2 (GFP-tagged) - Human growth factor receptor-bound protein 2 (GRB2), transcript variant 1||
|RC200469L1||Lenti ORF clone of Human growth factor receptor-bound protein 2 (GRB2), transcript variant 1, Myc-DDK-tagged||
|RC200469L2||Lenti ORF clone of Human growth factor receptor-bound protein 2 (GRB2), transcript variant 1, mGFP tagged||
|RC200469L3||Lenti ORF clone of Human growth factor receptor-bound protein 2 (GRB2), transcript variant 1, Myc-DDK-tagged||
|RC200469L4||Lenti ORF clone of Human growth factor receptor-bound protein 2 (GRB2), transcript variant 1, mGFP tagged||