MMP10 (NM_002425) Human Tagged ORF Clone

CAT#: RC200453

MMP10 (Myc-DDK-tagged)-Human matrix metallopeptidase 10 (stromelysin 2) (MMP10)

Product Images

Circular map for RC200453

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  Western blot validation of overexpression lysate (Cat# LY400867) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200453 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Western blot validation of overexpression lysate (Cat# LY400867) using an anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200453 using transfection reagent PEI

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MMP10
• Synonyms: SL-2; STMY2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200453 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGCATCTTGCATTCCTTGTGCTGTTGTGTCTGCCAGTCTGCTCTGCCTATCCTCTGAGTGGGGCAG
  CAAAAGAGGAGGACTCCAACAAGGATCTTGCCCAGCAATACCTAGAAAAGTACTACAACCTCGAAAAGGA
  TGTGAAACAGTTTAGAAGAAAGGACAGTAATCTCATTGTTAAAAAAATCCAAGGAATGCAGAAGTTCCTT
  GGGTTGGAGGTGACAGGGAAGCTAGACACTGACACTCTGGAGGTGATGCGCAAGCCCAGGTGTGGAGTTC
  CTGACGTTGGTCACTTCAGCTCCTTTCCTGGCATGCCGAAGTGGAGGAAAACCCACCTTACATACAGGAT
  TGTGAATTATACACCAGATTTGCCAAGAGATGCTGTTGATTCTGCCATTGAGAAAGCTCTGAAAGTCTGG
  GAAGAGGTGACTCCACTCACATTCTCCAGGCTGTATGAAGGAGAGGCTGATATAATGATCTCTTTTGCAG
  TTAAAGAACATGGAGACTTTTACTCTTTTGATGGCCCAGGACACAGTTTGGCTCATGCCTACCCACCTGG
  ACCTGGGCTTTATGGAGATATTCACTTTGATGATGATGAAAAATGGACAGAAGATGCATCAGGCACCAAT
  TTATTCCTCGTTGCTGCTCATGAACTTGGCCACTCCCTGGGGCTCTTTCACTCAGCCAACACTGAAGCTT
  TGATGTACCCACTCTACAACTCATTCACAGAGCTCGCCCAGTTCCGCCTTTCGCAAGATGATGTGAATGG
  CATTCAGTCTCTCTACGGACCTCCCCCTGCCTCTACTGAGGAACCCCTGGTGCCCACAAAATCTGTTCCT
  TCGGGATCTGAGATGCCAGCCAAGTGTGATCCTGCTTTGTCCTTCGATGCCATCAGCACTCTGAGGGGAG
  AATATCTGTTCTTTAAAGACAGATATTTTTGGCGAAGATCCCACTGGAACCCTGAACCTGAATTTCATTT
  GATTTCTGCATTTTGGCCCTCTCTTCCATCATATTTGGATGCTGCATATGAAGTTAACAGCAGGGACACC
  GTTTTTATTTTTAAAGGAAATGAGTTCTGGGCCATCAGAGGAAATGAGGTACAAGCAGGTTATCCAAGAG
  GCATCCATACCCTGGGTTTTCCTCCAACCATAAGGAAAATTGATGCAGCTGTTTCTGACAAGGAAAAGAA
  GAAAACATACTTCTTTGCAGCGGACAAATACTGGAGATTTGATGAAAATAGCCAGTCCATGGAGCAAGGC
  TTCCCTAGACTAATAGCTGATGACTTTCCAGGAGTTGAGCCTAAGGTTGATGCTGTATTACAGGCATTTG
  GATTTTTCTACTTCTTCAGTGGATCATCACAGTTTGAGTTTGACCCCAATGCCAGGATGGTGACACACAT
  ATTAAAGAGTAACAGCTGGTTACATTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200453 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MMHLAFLVLLCLPVCSAYPLSGAAKEEDSNKDLAQQYLEKYYNLEKDVKQFRRKDSNLIVKKIQGMQKFL
  GLEVTGKLDTDTLEVMRKPRCGVPDVGHFSSFPGMPKWRKTHLTYRIVNYTPDLPRDAVDSAIEKALKVW
  EEVTPLTFSRLYEGEADIMISFAVKEHGDFYSFDGPGHSLAHAYPPGPGLYGDIHFDDDEKWTEDASGTN
  LFLVAAHELGHSLGLFHSANTEALMYPLYNSFTELAQFRLSQDDVNGIQSLYGPPPASTEEPLVPTKSVP
  SGSEMPAKCDPALSFDAISTLRGEYLFFKDRYFWRRSHWNPEPEFHLISAFWPSLPSYLDAAYEVNSRDT
  VFIFKGNEFWAIRGNEVQAGYPRGIHTLGFPPTIRKIDAAVSDKEKKKTYFFAADKYWRFDENSQSMEQG
  FPRLIADDFPGVEPKVDAVLQAFGFFYFFSGSSQFEFDPNARMVTHILKSNSWLHC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002425
• ORF Size: 1428 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002425.3
• RefSeq Size: 1777 bp
• RefSeq ORF: 1431 bp
• Locus ID: 4319
• UniProt ID: P09238
• Cytogenetics: 11q22.2
• Protein Families: Druggable Genome, Protease
• MW: 54.2 kDa
• Gene Summary: This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This secreted protease breaks down fibronectin, laminin, elastin, proteoglycan core protein, gelatins, and several types of collagen. The gene is part of a cluster of MMP genes on chromosome 11. [provided by RefSeq, Jan 2016]