MMP10 (NM_002425) Human Tagged ORF Clone

CAT#: RC200453

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MMP10 (Myc-DDK-tagged)-Human matrix metallopeptidase 10 (stromelysin 2) (MMP10)



  "NM_002425" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 457.00

USD 686.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol MMP10
Synonyms SL-2; STMY2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC200453 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGCATCTTGCATTCCTTGTGCTGTTGTGTCTGCCAGTCTGCTCTGCCTATCCTCTGAGTGGGGCAG
CAAAAGAGGAGGACTCCAACAAGGATCTTGCCCAGCAATACCTAGAAAAGTACTACAACCTCGAAAAGGA
TGTGAAACAGTTTAGAAGAAAGGACAGTAATCTCATTGTTAAAAAAATCCAAGGAATGCAGAAGTTCCTT
GGGTTGGAGGTGACAGGGAAGCTAGACACTGACACTCTGGAGGTGATGCGCAAGCCCAGGTGTGGAGTTC
CTGACGTTGGTCACTTCAGCTCCTTTCCTGGCATGCCGAAGTGGAGGAAAACCCACCTTACATACAGGAT
TGTGAATTATACACCAGATTTGCCAAGAGATGCTGTTGATTCTGCCATTGAGAAAGCTCTGAAAGTCTGG
GAAGAGGTGACTCCACTCACATTCTCCAGGCTGTATGAAGGAGAGGCTGATATAATGATCTCTTTTGCAG
TTAAAGAACATGGAGACTTTTACTCTTTTGATGGCCCAGGACACAGTTTGGCTCATGCCTACCCACCTGG
ACCTGGGCTTTATGGAGATATTCACTTTGATGATGATGAAAAATGGACAGAAGATGCATCAGGCACCAAT
TTATTCCTCGTTGCTGCTCATGAACTTGGCCACTCCCTGGGGCTCTTTCACTCAGCCAACACTGAAGCTT
TGATGTACCCACTCTACAACTCATTCACAGAGCTCGCCCAGTTCCGCCTTTCGCAAGATGATGTGAATGG
CATTCAGTCTCTCTACGGACCTCCCCCTGCCTCTACTGAGGAACCCCTGGTGCCCACAAAATCTGTTCCT
TCGGGATCTGAGATGCCAGCCAAGTGTGATCCTGCTTTGTCCTTCGATGCCATCAGCACTCTGAGGGGAG
AATATCTGTTCTTTAAAGACAGATATTTTTGGCGAAGATCCCACTGGAACCCTGAACCTGAATTTCATTT
GATTTCTGCATTTTGGCCCTCTCTTCCATCATATTTGGATGCTGCATATGAAGTTAACAGCAGGGACACC
GTTTTTATTTTTAAAGGAAATGAGTTCTGGGCCATCAGAGGAAATGAGGTACAAGCAGGTTATCCAAGAG
GCATCCATACCCTGGGTTTTCCTCCAACCATAAGGAAAATTGATGCAGCTGTTTCTGACAAGGAAAAGAA
GAAAACATACTTCTTTGCAGCGGACAAATACTGGAGATTTGATGAAAATAGCCAGTCCATGGAGCAAGGC
TTCCCTAGACTAATAGCTGATGACTTTCCAGGAGTTGAGCCTAAGGTTGATGCTGTATTACAGGCATTTG
GATTTTTCTACTTCTTCAGTGGATCATCACAGTTTGAGTTTGACCCCAATGCCAGGATGGTGACACACAT
ATTAAAGAGTAACAGCTGGTTACATTGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC200453 protein sequence
Red=Cloning site Green=Tags(s)

MMHLAFLVLLCLPVCSAYPLSGAAKEEDSNKDLAQQYLEKYYNLEKDVKQFRRKDSNLIVKKIQGMQKFL
GLEVTGKLDTDTLEVMRKPRCGVPDVGHFSSFPGMPKWRKTHLTYRIVNYTPDLPRDAVDSAIEKALKVW
EEVTPLTFSRLYEGEADIMISFAVKEHGDFYSFDGPGHSLAHAYPPGPGLYGDIHFDDDEKWTEDASGTN
LFLVAAHELGHSLGLFHSANTEALMYPLYNSFTELAQFRLSQDDVNGIQSLYGPPPASTEEPLVPTKSVP
SGSEMPAKCDPALSFDAISTLRGEYLFFKDRYFWRRSHWNPEPEFHLISAFWPSLPSYLDAAYEVNSRDT
VFIFKGNEFWAIRGNEVQAGYPRGIHTLGFPPTIRKIDAAVSDKEKKKTYFFAADKYWRFDENSQSMEQG
FPRLIADDFPGVEPKVDAVLQAFGFFYFFSGSSQFEFDPNARMVTHILKSNSWLHC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_002425
ORF Size 1428 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002425.3
RefSeq Size 1777 bp
RefSeq ORF 1431 bp
Locus ID 4319
UniProt ID P09238
Cytogenetics 11q22.2
Protein Families Druggable Genome, Protease
MW 54.2 kDa
Gene Summary This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This secreted protease breaks down fibronectin, laminin, elastin, proteoglycan core protein, gelatins, and several types of collagen. The gene is part of a cluster of MMP genes on chromosome 11. [provided by RefSeq, Jan 2016]

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