PDZK1 (NM_002614) Human Tagged ORF Clone

CAT#: RC200444

PDZK1 (Myc-DDK-tagged)-Human PDZ domain containing 1 (PDZK1), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY400926) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200444 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PDZK1 protein (Cat# TP300444). The protein was produced from HEK293T cells transfected with PDZK1 cDNA clone (Cat# RC200444) using MegaTran 2.0 (Cat# TT210002).

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PDZK1 (Cat# RC200444, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDZK1 (Cat# TA813175)(1:500).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PDZK1
• Synonyms: CAP70; CLAMP; NHERF-3; NHERF3; PDZD1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200444 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCTCCACCTTCAACCCCCGAGAATGTAAACTGTCCAAGCAAGAAGGGCAAAACTATGGCTTCTTCC
  TGCGAATTGAGAAGGACACCGAGGGCCACCTGGTCCGGGTGGTTGAGAAGTGTAGCCCAGCAGAGAAGGC
  TGGCCTTCAAGATGGAGACAGAGTTCTTAGGATCAATGGTGTCTTTGTGGACAAAGAAGAACATATGCAG
  GTTGTGGATCTGGTCAGAAAGAGTGGGAATTCAGTGACTTTACTAGTTCTGGATGGGGATTCCTATGAGA
  AAGCAGTGAAAACACGGGTGGACTTGAAAGAGTTGGGTCAAAGTCAGAAGGAGCAAGGTTTGAGTGATAA
  TATACTTTCCCCTGTGATGAATGGAGGTGTGCAAACTTGGACCCAGCCCCGGCTCTGCTATCTCGTGAAG
  GAAGGAGGCAGCTATGGCTTCTCTCTGAAAACTGTCCAAGGTAAAAAGGGGGTGTACATGACTGATATTA
  CACCTCAAGGTGTGGCTATGAGAGCTGGAGTTCTGGCTGATGATCACTTGATTGAAGTGAATGGAGAGAA
  TGTAGAGGATGCCAGCCATGAGGAAGTGGTTGAAAAGGTGAAGAAGTCAGGAAGCCGTGTCATGTTCCTG
  CTGGTGGACAAAGAAACTGACAAGCGTCATGTTGAGCAGAAGATACAATTCAAAAGAGAAACAGCCAGTT
  TGAAACTGTTACCCCACCAGCCCCGAATTGTGGAGATGAAGAAAGGAAGCAATGGCTATGGTTTCTATCT
  GAGGGCAGGCTCAGAACAGAAAGGTCAAATCATCAAGGACATAGATTCTGGAAGTCCAGCAGAGGAGGCT
  GGCTTGAAGAACAATGATCTGGTAGTTGCTGTCAACGGCGAGTCTGTGGAAACCCTGGATCATGACAGTG
  TGGTAGAAATGATTAGAAAGGGTGGAGATCAGACTTCACTGTTGGTGGTAGACAAAGAGACGGACAACAT
  GTACAGACTGGCTCATTTTTCTCCATTTCTCTACTATCAAAGTCAAGAACTGCCCAATGGCTCTGTCAAG
  GAGGCTCCAGCTCCTACTCCCACTTCTCTGGAAGTCTCAAGTCCACCAGATACTACAGAGGAAGTAGATC
  ATAAGCCTAAACTCTGCAGGCTGGCTAAAGGTGAAAATGGCTATGGCTTTCACTTAAATGCGATTCGGGG
  TCTGCCAGGCTCATTCATCAAAGAGGTACAGAAGGGCGGTCCTGCTGACTTGGCTGGGCTAGAGGATGAG
  GATGTCATCATTGAAGTGAATGGGGTGAATGTGCTAGATGAACCCTATGAGAAGGTGGTGGATAGAATCC
  AGAGCAGTGGGAAGAATGTCACACTTCTAGTCTGTGGAAAGAAGGCCTATGATTATTTCCAAGCTAAGAA
  AATCCCTATTGTTTCCTCCCTGGCTGATCCACTTGACACCCCTCCAGATTCTAAAGAAGGAATAGTGGTG
  GAGTCAAACCATGACTCGCACATGGCAAAAGAACGGGCCCACAGTACAGCCTCACATTCTTCTTCCAATT
  CTGAAGATACAGAGATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200444 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTSTFNPRECKLSKQEGQNYGFFLRIEKDTEGHLVRVVEKCSPAEKAGLQDGDRVLRINGVFVDKEEHMQ
  VVDLVRKSGNSVTLLVLDGDSYEKAVKTRVDLKELGQSQKEQGLSDNILSPVMNGGVQTWTQPRLCYLVK
  EGGSYGFSLKTVQGKKGVYMTDITPQGVAMRAGVLADDHLIEVNGENVEDASHEEVVEKVKKSGSRVMFL
  LVDKETDKRHVEQKIQFKRETASLKLLPHQPRIVEMKKGSNGYGFYLRAGSEQKGQIIKDIDSGSPAEEA
  GLKNNDLVVAVNGESVETLDHDSVVEMIRKGGDQTSLLVVDKETDNMYRLAHFSPFLYYQSQELPNGSVK
  EAPAPTPTSLEVSSPPDTTEEVDHKPKLCRLAKGENGYGFHLNAIRGLPGSFIKEVQKGGPADLAGLEDE
  DVIIEVNGVNVLDEPYEKVVDRIQSSGKNVTLLVCGKKAYDYFQAKKIPIVSSLADPLDTPPDSKEGIVV
  ESNHDSHMAKERAHSTASHSSSNSEDTEM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002614
• ORF Size: 1557 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002614.4
• RefSeq Size: 2301 bp
• RefSeq ORF: 1560 bp
• Locus ID: 5174
• UniProt ID: Q5T2W1
• Cytogenetics: 1q21.1
• Domains: PDZ
• MW: 57.1 kDa
• Gene Summary: This gene encodes a PDZ domain-containing scaffolding protein. PDZ domain-containing molecules bind to and mediate the subcellular localization of target proteins. The encoded protein mediates the localization of cell surface proteins and plays a critical role in cholesterol metabolism by regulating the HDL receptor, scavenger receptor class B type 1. Single nucleotide polymorphisms in this gene may be associated with metabolic syndrome, and overexpression of this gene may play a role in drug resistance of multiple myeloma. Pseudogenes of this gene are located on the long arm of chromosome 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]