PLCG 2 (PLCG2) (NM_002661) Human Tagged ORF Clone
Lenti ORF clone of Human phospholipase C, gamma 2 (phosphatidylinositol-specific) (PLCG2), Myc-DDK-tagged
|Product Name||PLCG 2 (PLCG2) (NM_002661) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||APLAID; FCAS3; PLC-gamma-2; PLC-IV|
|E. coli Selection||Chloramphenicol (34 ug/mL)|
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene|
|ORF Size||3795 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||8707 bp|
|RefSeq ORF||3798 bp|
|Domains||C2, PI-PLC-X, SH2, SH3, PI-PLC-Y, PH|
|Protein Families||Druggable Genome|
|Protein Pathways||B cell receptor signaling pathway, Calcium signaling pathway, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Glioma, Inositol phosphate metabolism, Leukocyte transendothelial migration, Metabolic pathways, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pathways in cancer, Phosphatidylinositol signaling system, VEGF signaling pathway, Vibrio cholerae infection|
|Gene Summary||The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3. [provided by RefSeq, Mar 2014]|
|The use of this cDNA Clones has been cited in the following citations:|
The Alzheimer′ s disease-associated protective Plcγ2-P522R variant promotes beneficial microglial functions
,Takalo, M;Wittrahm, R;Wefers, B;Parhizkar, S;,
|cDNA Clone Resources|
|SC118495||PLCG2 (untagged)-Human phospholipase C, gamma 2 (phosphatidylinositol-specific) (PLCG2)||
|SC318575||PLCG2 (untagged)-Human phospholipase C, gamma 2 (phosphatidylinositol-specific) (PLCG2)||
|RC200442||PLCG2 (Myc-DDK-tagged)-Human phospholipase C, gamma 2 (phosphatidylinositol-specific) (PLCG2)||
|RG200442||PLCG2 (GFP-tagged) - Human phospholipase C, gamma 2 (phosphatidylinositol-specific) (PLCG2)||
|RC200442L2||Lenti ORF clone of Human phospholipase C, gamma 2 (phosphatidylinositol-specific) (PLCG2), mGFP tagged||
|RC200442L3||Lenti ORF clone of Human phospholipase C, gamma 2 (phosphatidylinositol-specific) (PLCG2), Myc-DDK-tagged||
|RC200442L4||Lenti ORF clone of Human phospholipase C, gamma 2 (phosphatidylinositol-specific) (PLCG2), mGFP tagged||