Rab2 (RAB2A) (NM_002865) Human Tagged ORF Clone
RAB2A (Myc-DDK-tagged)-Human RAB2A, member RAS oncogene family (RAB2A)
|Type||Human Tagged ORF Clone|
|E. coli Selection||Kanamycin (25 ug/mL)|
|Mammalian Cell Selection||Neomycin|
>RC200433 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC200433 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||636 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||3812 bp|
|RefSeq ORF||639 bp|
|Protein Families||Druggable Genome|
|Gene Summary||The protein encoded by this gene belongs to the Rab family, members of which are small molecular weight guanosine triphosphatases (GTPases) that contain highly conserved domains involved in GTP binding and hydrolysis. The Rabs are membrane-bound proteins, involved in vesicular fusion and trafficking. This protein is a resident of pre-Golgi intermediates, and is required for protein transport from the endoplasmic reticulum (ER) to the Golgi complex. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]|
|cDNA Clone Resources|
|RC200433L1||Lenti ORF clone of Human RAB2A, member RAS oncogene family (RAB2A), Myc-DDK-tagged||
|RC200433L2||Lenti ORF clone of Human RAB2A, member RAS oncogene family (RAB2A), mGFP tagged||
|RC200433L3||Lenti ORF clone of Human RAB2A, member RAS oncogene family (RAB2A), Myc-DDK-tagged||
|RC200433L4||Lenti ORF clone of Human RAB2A, member RAS oncogene family (RAB2A), mGFP tagged||
|RG200433||RAB2A (GFP-tagged) - Human RAB2A, member RAS oncogene family (RAB2A)||
|SC118348||RAB2A (untagged)-Human RAB2A, member RAS oncogene family (RAB2A)||