VAMP8 (NM_003761) Human Tagged ORF Clone

CAT#: RC200405

VAMP8 (Myc-DDK-tagged)-Human vesicle-associated membrane protein 8 (endobrevin) (VAMP8)

Product Images

Western blot validation of overexpression lysate (Cat# LY401238) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200405 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified VAMP8 protein (Cat# TP300405). The protein was produced from HEK293T cells transfected with VAMP8 cDNA clone (Cat# RC200405) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VAMP8
• Synonyms: EDB; VAMP-8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200405 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAAGCCAGTGAAGGTGGAGGAAATGATCGTGTGCGGAACCTGCAAAGTGAGGTGGAGGGAGTTA
  AGAATATTATGACCCAGAATGTGGAGCGGATCCTGGCCCGGGGGGAAAACTTGGAACATCTCCGCAACAA
  GACAGAGGATCTGGAAGCCACATCTGAGCACTTCAAGACGACATCGCAGAAGGTGGCTCGGAAATTCTGG
  TGGAAGAACGTGAAGATGATTGTCCTTATCTGCGTGATTGTTTTTATCATCATCCTCTTCATTGTGCTCT
  TTGCCACTGGTGCCTTCTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200405 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEEASEGGGNDRVRNLQSEVEGVKNIMTQNVERILARGENLEHLRNKTEDLEATSEHFKTTSQKVARKFW
  WKNVKMIVLICVIVFIIILFIVLFATGAFS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003761
• ORF Size: 300 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003761.2, NP_003752.2
• RefSeq Size: 790 bp
• RefSeq ORF: 303 bp
• Locus ID: 8673
• UniProt ID: Q9BV40
• Cytogenetics: 2p11.2
• Domains: synaptobrevin
• Protein Families: Stem cell - Pluripotency, Transmembrane
• Protein Pathways: SNARE interactions in vesicular transport
• MW: 11.4 kDa
• Gene Summary: This gene encodes an integral membrane protein that belongs to the synaptobrevin/vesicle-associated membrane protein subfamily of soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs). The encoded protein is involved in the fusion of synaptic vesicles with the presynaptic membrane.[provided by RefSeq, Jun 2010]