OXSR1 (NM_005109) Human Tagged ORF Clone

CAT#: RC200396

OXSR1 (Myc-DDK-tagged)-Human oxidative-stress responsive 1 (OXSR1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY OXSR1 (Cat# RC200396, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OXSR1(Cat# TA500392). Positive lysates LY401573 (100ug) and LC401573 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY401573) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200396 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified OXSR1 protein (Cat# TP300396). The protein was produced from HEK293T cells transfected with OXSR1 cDNA clone (Cat# RC200396) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: OXSR1
• Synonyms: OSR1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200396 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGAGGACTCGAGCGCCCTGCCCTGGTCCATCAACAGGGACGATTACGAGCTGCAGGAGGTGATCG
  GGAGTGGAGCAACTGCTGTAGTCCAAGCAGCTTATTGTGCCCCTAAAAAGGAGAAAGTGGCAATCAAACG
  GATAAACCTTGAGAAATGTCAAACTAGCATGGATGAACTCCTGAAAGAAATTCAAGCCATGAGTCAATGC
  CATCATCCTAATATTGTATCTTACTACACATCTTTTGTGGTAAAAGATGAGCTGTGGCTTGTCATGAAGC
  TGCTAAGTGGAGGTTCTGTTCTGGATATTATTAAGCACATTGTGGCAAAAGGGGAACACAAAAGTGGAGT
  CCTAGATGAATCTACCATTGCTACGATACTCCGAGAAGTACTGGAAGGGCTGGAATATCTGCATAAAAAT
  GGACAGATCCACAGAGATGTGAAAGCTGGAAACATTCTTCTTGGAGAAGATGGCTCAGTACAGATTGCAG
  ACTTTGGGGTTAGTGCTTTTTTAGCAACTGGTGGTGATATTACCCGAAATAAAGTGAGAAAGACCTTTGT
  TGGCACCCCTTGTTGGATGGCACCTGAAGTTATGGAACAGGTCCGTGGTTATGATTTCAAAGCTGATATT
  TGGAGTTTTGGAATTACAGCAATTGAATTGGCTACAGGGGCGGCTCCTTATCATAAATATCCACCAATGA
  AGGTTTTAATGCTGACACTGCAGAACGATCCTCCTTCTTTGGAAACTGGTGTTCAAGATAAAGAAATGCT
  GAAAAAATATGGAAAATCATTTAGAAAAATGATTTCATTGTGCCTTCAAAAAGATCCAGAAAAAAGACCA
  ACAGCAGCAGAACTATTAAGGCACAAATTTTTCCAGAAAGCAAAGAATAAAGAATTTCTTCAAGAAAAAA
  CATTGCAGAGAGCACCAACCATTTCTGAAAGAGCAAAAAAGGTTCGGAGAGTACCAGGTTCCAGTGGGCG
  TCTTCATAAGACAGAGGATGGAGGCTGGGAGTGGAGTGATGATGAATTTGATGAAGAAAGTGAGGAAGGG
  AAAGCAGCAATTTCACAACTCAGGTCTCCCCGAGTGAAAGAATCAATATCAAATTCTGAGCTCTTTCCAA
  CAACTGATCCTGTGGGTACTTTGCTCCAAGTTCCAGAACAGATCTCTGCTCATCTACCTCAGCCAGCTGG
  GCAGATTGCTACACAGCCAACTCAAGTCTCTCTCCCACCCACCGCAGAGCCAGCAAAAACAGCTCAGGCT
  TTGTCTTCAGGATCAGGTTCACAAGAAACCAAGATCCCAATCAGTCTAGTACTAAGATTAAGGAATTCCA
  AAAAAGAACTAAATGATATTCGATTTGAATTTACTCCTGGGAGAGATACAGCAGAGGGTGTCTCTCAGGA
  ACTCATTTCTGCTGGCCTGGTCGACGGAAGGGATTTAGTAATAGTGGCAGCTAATTTGCAGAAAATTGTG
  GAAGAACCTCAGTCAAATCGATCTGTCACTTTCAAACTGGCATCTGGTGTCGAAGGCTCAGATATTCCTG
  ATGATGGTAAACTGATAGGATTTGCCCAGCTCAGCATCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200396 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSEDSSALPWSINRDDYELQEVIGSGATAVVQAAYCAPKKEKVAIKRINLEKCQTSMDELLKEIQAMSQC
  HHPNIVSYYTSFVVKDELWLVMKLLSGGSVLDIIKHIVAKGEHKSGVLDESTIATILREVLEGLEYLHKN
  GQIHRDVKAGNILLGEDGSVQIADFGVSAFLATGGDITRNKVRKTFVGTPCWMAPEVMEQVRGYDFKADI
  WSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPPSLETGVQDKEMLKKYGKSFRKMISLCLQKDPEKRP
  TAAELLRHKFFQKAKNKEFLQEKTLQRAPTISERAKKVRRVPGSSGRLHKTEDGGWEWSDDEFDEESEEG
  KAAISQLRSPRVKESISNSELFPTTDPVGTLLQVPEQISAHLPQPAGQIATQPTQVSLPPTAEPAKTAQA
  LSSGSGSQETKIPISLVLRLRNSKKELNDIRFEFTPGRDTAEGVSQELISAGLVDGRDLVIVAANLQKIV
  EEPQSNRSVTFKLASGVEGSDIPDDGKLIGFAQLSIS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005109
• ORF Size: 1581 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005109.3
• RefSeq Size: 4525 bp
• RefSeq ORF: 1584 bp
• Locus ID: 9943
• UniProt ID: O95747
• Cytogenetics: 3p22.2
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• MW: 58 kDa
• Gene Summary: The product of this gene belongs to the Ser/Thr protein kinase family of proteins. It regulates downstream kinases in response to environmental stress, and may play a role in regulating the actin cytoskeleton. [provided by RefSeq, Jul 2008]