Chemerin (RARRES2) (NM_002889) Human Tagged ORF Clone

CAT#: RC200383

RARRES2 (Myc-DDK-tagged)-Human retinoic acid receptor responder (tazarotene induced) 2 (RARRES2)

Product Images

Western blot validation of overexpression lysate (Cat# LY401013) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200383 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified RARRES2 protein (Cat# TP300383). The protein was produced from HEK293T cells transfected with RARRES2 cDNA clone (Cat# RC200383) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RARRES2
• Synonyms: HP10433; TIG2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200383 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGACGGCTGCTGATCCCTCTGGCCCTGTGGCTGGGCGCGGTGGGCGTGGGCGTCGCCGAGCTCACGG
  AAGCCCAGCGCCGGGGCCTGCAGGTGGCCCTGGAGGAATTTCACAAGCACCCGCCCGTGCAGTGGGCCTT
  CCAGGAGACCAGTGTGGAGAGCGCCGTGGACACGCCCTTCCCAGCTGGAATATTTGTGAGGCTGGAATTT
  AAGCTGCAGCAGACAAGCTGCCGGAAGAGGGACTGGAAGAAACCCGAGTGCAAAGTCAGGCCCAATGGGA
  GGAAACGGAAATGCCTGGCCTGCATCAAACTGGGCTCTGAGGACAAAGTTCTGGGCCGGTTGGTCCACTG
  CCCCATAGAGACCCAAGTTCTGCGGGAGGCTGAGGAGCACCAGGAGACCCAGTGCCTCAGGGTGCAGCGG
  GCTGGTGAGGACCCCCACAGCTTCTACTTCCCTGGACAGTTCGCCTTCTCCAAGGCCCTGCCCCGCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200383 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRRLLIPLALWLGAVGVGVAELTEAQRRGLQVALEEFHKHPPVQWAFQETSVESAVDTPFPAGIFVRLEF
  KLQQTSCRKRDWKKPECKVRPNGRKRKCLACIKLGSEDKVLGRLVHCPIETQVLREAEEHQETQCLRVQR
  AGEDPHSFYFPGQFAFSKALPRS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002889
• ORF Size: 489 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002889.4
• RefSeq Size: 767 bp
• RefSeq ORF: 492 bp
• Locus ID: 5919
• UniProt ID: Q99969
• Cytogenetics: 7q36.1
• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Secreted Protein
• MW: 18.6 kDa
• Gene Summary: This gene encodes a secreted chemotactic protein that initiates chemotaxis via the ChemR23 G protein-coupled seven-transmembrane domain ligand. Expression of this gene is upregulated by the synthetic retinoid tazarotene and occurs in a wide variety of tissues. The active protein has several roles, including that as an adipokine and as an antimicrobial protein with activity against bacteria and fungi. [provided by RefSeq, Nov 2014]