BUB3 (NM_004725) Human Tagged ORF Clone
CAT#: RC200376
BUB3 (Myc-DDK-tagged)-Human budding uninhibited by benzimidazoles 3 homolog (yeast) (BUB3), transcript variant 1
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_004725" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | BUB3 |
Synonyms | BUB3L; hBUB3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC200376 representing NM_004725
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCGGTTCTAACGAGTTCAAGCTGAACCAGCCACCCGAGGATGGCATCTCCTCCGTGAAGTTCAGCC CCAACACCTCCCAGTTCCTGCTTGTCTCCTCCTGGGACACGTCCGTGCGTCTCTACGATGTGCCGGCCAA CTCCATGCGGCTCAAGTACCAGCACACCGGCGCCGTCCTGGACTGCGCCTTCTACGATCCAACGCATGCC TGGAGTGGAGGACTAGATCATCAATTGAAAATGCATGATTTGAACACTGATCAAGAAAATCTTGTTGGGA CCCATGATGCCCCTATCAGATGTGTTGAATACTGTCCAGAAGTGAATGTGATGGTCACTGGAAGTTGGGA TCAGACAGTTAAACTGTGGGATCCCAGAACTCCTTGTAATGCTGGGACCTTCTCTCAGCCTGAAAAGGTA TATACCCTCTCAGTGTCTGGAGACCGGCTGATTGTGGGAACAGCAGGCCGCAGAGTGTTGGTGTGGGACT TACGGAACATGGGTTACGTGCAGCAGCGCAGGGAGTCCAGCCTGAAATACCAGACTCGCTGCATACGAGC GTTTCCAAACAAGCAGGGTTATGTATTAAGCTCTATTGAAGGCCGAGTGGCAGTTGAGTATTTGGACCCA AGCCCTGAGGTACAGAAGAAGAAGTATGCCTTCAAATGTCACAGACTAAAAGAAAATAATATTGAGCAGA TTTACCCAGTCAATGCCATTTCTTTTCACAATATCCACAATACATTTGCCACAGGTGGTTCTGATGGCTT TGTAAATATTTGGGATCCATTTAACAAAAAGCGACTGTGCCAATTCCATCGGTACCCCACGAGCATCGCA TCACTTGCCTTCAGTAATGATGGGACTACGCTTGCAATAGCGTCATCATATATGTATGAAATGGATGACA CAGAACATCCTGAAGATGGTATCTTCATTCGCCAAGTGACAGATGCAGAAACAAAACCCAAGTCACCATG TACT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200376 representing NM_004725
Red=Cloning site Green=Tags(s) MTGSNEFKLNQPPEDGISSVKFSPNTSQFLLVSSWDTSVRLYDVPANSMRLKYQHTGAVLDCAFYDPTHA WSGGLDHQLKMHDLNTDQENLVGTHDAPIRCVEYCPEVNVMVTGSWDQTVKLWDPRTPCNAGTFSQPEKV YTLSVSGDRLIVGTAGRRVLVWDLRNMGYVQQRRESSLKYQTRCIRAFPNKQGYVLSSIEGRVAVEYLDP SPEVQKKKYAFKCHRLKENNIEQIYPVNAISFHNIHNTFATGGSDGFVNIWDPFNKKRLCQFHRYPTSIA SLAFSNDGTTLAIASSYMYEMDDTEHPEDGIFIRQVTDAETKPKSPCT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_004725 |
ORF Size | 984 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_004725.4 |
RefSeq Size | 2659 bp |
RefSeq ORF | 987 bp |
Locus ID | 9184 |
UniProt ID | O43684 |
Cytogenetics | 10q26.13 |
Domains | WD40 |
Protein Families | Druggable Genome |
Protein Pathways | Cell cycle |
MW | 37 kDa |
Gene Summary | This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC200376L3 | Lenti-ORF clone of BUB3 (Myc-DDK-tagged)-Human budding uninhibited by benzimidazoles 3 homolog (yeast) (BUB3), transcript variant 1 |
USD 600.00 |
|
RC200376L4 | Lenti-ORF clone of BUB3 (mGFP-tagged)-Human budding uninhibited by benzimidazoles 3 homolog (yeast) (BUB3), transcript variant 1 |
USD 600.00 |
|
RG200376 | BUB3 (tGFP-tagged) - Human budding uninhibited by benzimidazoles 3 homolog (yeast) (BUB3), transcript variant 1 |
USD 500.00 |
|
SC319420 | BUB3 (untagged)-Human budding uninhibited by benzimidazoles 3 homolog (yeast) (BUB3), transcript variant 1 |
USD 300.00 |
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