PAFAH2 (NM_000437) Human Tagged ORF Clone

CAT#: RC200355

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PAFAH2 (Myc-DDK-tagged)-Human platelet-activating factor acetylhydrolase 2, 40kDa (PAFAH2)



Reconstitution Protocol

USD 457.00

USD 686.00

In Stock*

Size
    • 10 ug

Product images

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol PAFAH2
Synonyms HSD-PLA2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC200355 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGGTCAACCAGTCTGTGGGCTTTCCACCTGTCACAGGACCCCACCTCGTAGGCTGTGGGGATGTGA
TGGAGGGTCAGAATCTCCAGGGGAGCTTCTTTCGACTCTTCTACCCCTGCCAAAAGGCAGAGGAGACCAT
GGAGCAGCCCCTGTGGATTCCCCGCTATGAGTACTGCACTGGCCTGGCCGAGTACCTGCAGTTTAATAAG
CGCTGCGGGGGCTTGCTGTTCAACCTGGCGGTGGGATCTTGTCGCCTGCCTGTTAGCTGGAATGGCCCCT
TTAAGACAAAGGACTCTGGATACCCCTTGATCATCTTCTCCCATGGCCTAGGAGCCTTCAGGACTTTGTA
TTCAGCCTTCTGCATGGAGCTGGCCTCACGTGGCTTTGTGGTTGCTGTGCCAGAGCACAGGGACCGGTCA
GCGGCAACCACCTATTTCTGCAAGCAGGCCCCAGAAGAGAACCAGCCCACCAATGAATCGCTGCAGGAGG
AATGGATCCCTTTCCGTCGAGTTGAGGAAGGGGAGAAGGAATTTCATGTTCGGAATCCCCAGGTGCATCA
GCGGGTAAGCGAGTGTTTACGGGTGTTGAAGATCCTGCAAGAGGTCACTGCTGGGCAGACTGTCTTCAAC
ATCTTGCCTGGTGGCTTGGATCTGATGACTTTGAAGGGCAACATTGACATGAGCCGTGTGGCTGTGATGG
GACATTCATTTGGAGGGGCCACAGCTATTCTGGCTTTGGCCAAGGAGACCCAATTTCGGTGTGCGGTGGC
TCTGGATGCTTGGATGTTTCCTCTGGAACGTGACTTTTACCCCAAGGCCCGAGGACCTGTGTTCTTTATC
AATACTGAGAAATTCCAGACAATGGAGAGTGTCAATTTGATGAAGAAGATATGTGCCCAGCATGAACAGT
CTAGGATCATAACCGTTCTTGGTTCTGTTCATCGGAGTCAAACTGACTTTGCTTTTGTGACTGGCAACTT
GATTGGTAAATTCTTCTCCACTGAAACCCGTGGGAGCCTGGACCCCTATGAAGGGCAGGAGGTTATGGTA
CGGGCCATGTTGGCCTTCCTGCAGAAGCACCTCGACCTGAAAGAAGACTATAATCAATGGAACAACCTTA
TTGAAGGCATTGGACCGTCGCTCACCCCAGGGGCCCCCCACCATCTGTCCAGCCTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC200355 protein sequence
Red=Cloning site Green=Tags(s)

MGVNQSVGFPPVTGPHLVGCGDVMEGQNLQGSFFRLFYPCQKAEETMEQPLWIPRYEYCTGLAEYLQFNK
RCGGLLFNLAVGSCRLPVSWNGPFKTKDSGYPLIIFSHGLGAFRTLYSAFCMELASRGFVVAVPEHRDRS
AATTYFCKQAPEENQPTNESLQEEWIPFRRVEEGEKEFHVRNPQVHQRVSECLRVLKILQEVTAGQTVFN
ILPGGLDLMTLKGNIDMSRVAVMGHSFGGATAILALAKETQFRCAVALDAWMFPLERDFYPKARGPVFFI
NTEKFQTMESVNLMKKICAQHEQSRIITVLGSVHRSQTDFAFVTGNLIGKFFSTETRGSLDPYEGQEVMV
RAMLAFLQKHLDLKEDYNQWNNLIEGIGPSLTPGAPHHLSSL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_000437
ORF Size 1176 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000437.4
RefSeq Size 3581 bp
RefSeq ORF 1179 bp
Locus ID 5051
UniProt ID Q99487
Cytogenetics 1p36.11
Domains PAF-AH_p_II
Protein Families Druggable Genome
Protein Pathways Ether lipid metabolism, Metabolic pathways
MW 44 kDa
Gene Summary This gene encodes platelet-activating factor acetylhydrolase isoform 2, a single-subunit intracellular enzyme that catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor (identified as 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine). However, this lipase exhibits a broader substrate specificity than simply platelet activating factor. Two other isoforms of intracellular platelet-activating factor acetylhydrolase exist, and both are multi-subunit enzymes. Additionally, there is a single-subunit serum isoform of this enzyme. [provided by RefSeq, Jul 2008]
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