KCNJ8 (NM_004982) Human Tagged ORF Clone

CAT#: RC200340

KCNJ8 (Myc-DDK-tagged)-Human potassium inwardly-rectifying channel, subfamily J, member 8 (KCNJ8)

Product Images

Circular map for RC200340

• 

  Western blot validation of overexpression lysate (Cat# LY417611) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200340 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KCNJ8
• Synonyms: KIR6.1; uKATP-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200340 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGGCCAGAAAGAGTATCATCCCGGAGGAGTATGTGCTGGCGCGCATCGCCGCAGAGAACCTGCGCA
  AGCCGCGCATCCGAGACCGCCTCCCCAAAGCCCGCTTCATCGCCAAGAGCGGGGCCTGCAACCTGGCGCA
  TAAGAACATCCGTGAGCAAGGACGCTTTCTACAGGACATCTTCACCACCTTGGTGGACCTGAAATGGCGC
  CACACGCTGGTCATCTTTACCATGTCCTTCCTCTGCAGCTGGCTGCTCTTCGCTATCATGTGGTGGCTGG
  TGGCCTTTGCCCATGGGGACATCTATGCTTACATGGAGAAAAGTGGAATGGAGAAAAGTGGTTTGGAGTC
  CACTGTGTGTGTGACTAATGTCAGGTCTTTCACTTCTGCTTTTCTCTTCTCCATTGAAGTTCAAGTTACC
  ATTGGGTTTGGAGGGAGGATGATGACAGAGGAATGCCCTTTGGCCATCACGGTTTTGATTCTCCAGAATA
  TTGTGGGTTTGATCATCAATGCAGTCATGTTAGGCTGCATTTTCATGAAAACAGCTCAGGCTCACAGAAG
  GGCAGAAACTTTGATTTTCAGCCGCCATGCTGTGATTGCCGTCCGAAATGGCAAGCTGTGCTTCATGTTC
  CGAGTGGGTGACCTGAGGAAAAGCATGATCATTAGTGCCTCTGTGCGCATCCAGGTGGTCAAGAAAACAA
  CTACACCTGAAGGGGAGGTGGTTCCTATTCACCAACTGGACATTCCTGTTGATAACCCAATCGAGAGCAA
  TAACATTTTTCTGGTGGCCCCTTTGATCATCTGCCACGTGATTGACAAGCGCAGTCCCCTGTATGACATC
  TCAGCAACTGACCTGGCCAACCAAGACTTGGAGGTCATAGTTATTCTGGAAGGAGTGGTTGAAACTACTG
  GCATCACCACACAAGCACGAACCTCCTACATTGCTGAGGAGATCCAATGGGGCCACCGCTTTGTGTCCAT
  TGTGACTGAGGAAGAAGGAGTGTATTCTGTGGATTACTCCAAATTTGGCAACACTGTTAAAGTAGCTGCT
  CCACGGTGCAGTGCCCGAGAGCTGGATGAGAAACCTTCCATCCTTATTCAGACCCTCCAAAAGAGTGAAC
  TGTCTCATCAAAATTCTCTGAGGAAGCGCAACTCCATGAGAAGAAACAATTCCATGAGGAGGAACAATTC
  TATCCGAAGGAACAATTCTTCCCTCATGGTACCAAAGGTGCAATTTATGACTCCAGAAGGAAATCAAAAC
  ACATCGGAATCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200340 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLARKSIIPEEYVLARIAAENLRKPRIRDRLPKARFIAKSGACNLAHKNIREQGRFLQDIFTTLVDLKWR
  HTLVIFTMSFLCSWLLFAIMWWLVAFAHGDIYAYMEKSGMEKSGLESTVCVTNVRSFTSAFLFSIEVQVT
  IGFGGRMMTEECPLAITVLILQNIVGLIINAVMLGCIFMKTAQAHRRAETLIFSRHAVIAVRNGKLCFMF
  RVGDLRKSMIISASVRIQVVKKTTTPEGEVVPIHQLDIPVDNPIESNNIFLVAPLIICHVIDKRSPLYDI
  SATDLANQDLEVIVILEGVVETTGITTQARTSYIAEEIQWGHRFVSIVTEEEGVYSVDYSKFGNTVKVAA
  PRCSARELDEKPSILIQTLQKSELSHQNSLRKRNSMRRNNSMRRNNSIRRNNSSLMVPKVQFMTPEGNQN
  TSES
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004982
• ORF Size: 1272 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004982.4
• RefSeq Size: 2406 bp
• RefSeq ORF: 1275 bp
• Locus ID: 3764
• UniProt ID: Q15842
• Cytogenetics: 12p12.1
• Domains: IRK
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• MW: 48 kDa
• Gene Summary: Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, is controlled by G-proteins. Defects in this gene may be a cause of J-wave syndromes and sudden infant death syndrome (SIDS). [provided by RefSeq, May 2012]