HCLS1 (NM_005335) Human Tagged ORF Clone

CAT#: RC200329

HCLS1 (Myc-DDK-tagged)-Human hematopoietic cell-specific Lyn substrate 1 (HCLS1)

Product Images

Circular map for RC200329

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  Western blot validation of overexpression lysate (Cat# LY417367) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200329 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified HCLS1 protein (Cat# TP300329). The protein was produced from HEK293T cells transfected with HCLS1 cDNA clone (Cat# RC200329) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HCLS1
• Synonyms: CTTNL; HS1; lckBP1; p75
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200329 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGAAGTCTGTAGTGGGCCATGATGTGTCTGTTTCCGTGGAGACCCAGGGTGATGATTGGGACACAG
  ATCCTGACTTTGTGAATGACATCTCTGAAAAGGAGCAACGATGGGGAGCCAAGACCATCGAGGGGTCTGG
  ACGCACAGAACACATCAACATCCACCAGCTGAGGAACAAAGTATCAGAGGAGCATGATGTTCTCAGGAAG
  AAAGAGATGGAGTCAGGGCCCAAAGCATCCCATGGCTATGGAGGTCGGTTTGGAGTAGAAAGAGACCGAA
  TGGACAAGAGTGCAGTGGGCCATGAGTATGTTGCCGAGGTGGAGAAGCACTCTTCTCAGACGGATGCTGC
  CAAAGGCTTTGGGGGCAAGTACGGAGTTGAGAGGGACAGGGCAGACAAGTCAGCAGTCGGCTTTGATTAT
  AAAGGAGAAGTGGAGAAGCATACATCTCAGAAAGATTACTCTCGTGGCTTTGGTGGCCGGTACGGGGTGG
  AGAAGGATAAATGGGACAAAGCAGCTCTGGGATATGACTACAAGGGAGAGACGGAGAAACACGAGTCCCA
  GAGAGATTATGCCAAGGGCTTTGGTGGCCAGTATGGAATCCAGAAGGACCGAGTGGATAAGAGCGCTGTC
  GGCTTCAATGAAATGGAGGCCCCGACCACAGCTTATAAGAAGACGACGCCCATAGAAGCCGCTTCTAGTG
  GTGCCCGTGGGCTGAAGGCGAAATTTGAGTCCATGGCTGAGGAGAAGAGGAAGCGAGAGGAAGAGGAGAA
  GGCACAGCAGGTGGCCAGGAGGCAACAGGAGCGAAAGGCTGTGACAAAGAGGAGCCCTGAGGCTCCACAG
  CCAGTGATAGCTATGGAAGAGCCAGCAGTACCGGCCCCACTGCCCAAGAAAATCTCCTCAGAGGCCTGGC
  CTCCAGTTGGGACTCCTCCATCATCAGAGTCTGAGCCTGTGAGAACCAGCAGGGAACACCCAGTGCCCTT
  GCTGCCCATTAGGCAGACTCTCCCGGAGGACAATGAGGAGCCCCCAGCTCTGCCCCCTAGGACTCTGGAA
  GGCCTCCAGGTGGAGGAAGAGCCAGTGTACGAAGCAGAGCCTGAGCCTGAGCCCGAGCCTGAGCCCGAGC
  CTGAGAATGACTATGAGGACGTTGAGGAGATGGACAGGCATGAGCAGGAGGATGAACCAGAGGGGGACTA
  TGAGGAGGTGCTCGAGCCTGAAGATTCTTCTTTTTCTTCTGCTCTGGCTGGATCATCAGGCTGCCCGGCT
  GGGGCTGGGGCTGGGGCTGTGGCTCTGGGGATCTCAGCTGTGGCTCTATATGATTACCAAGGAGAGGGAA
  GTGATGAGCTTTCCTTTGATCCGGACGACGTAATCACTGACATTGAGATGGTGGACGAGGGCTGGTGGCG
  GGGACGTTGCCATGGCCACTTTGGACTCTTCCCTGCAAATTATGTCAAGCTTCTGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200329 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MWKSVVGHDVSVSVETQGDDWDTDPDFVNDISEKEQRWGAKTIEGSGRTEHINIHQLRNKVSEEHDVLRK
  KEMESGPKASHGYGGRFGVERDRMDKSAVGHEYVAEVEKHSSQTDAAKGFGGKYGVERDRADKSAVGFDY
  KGEVEKHTSQKDYSRGFGGRYGVEKDKWDKAALGYDYKGETEKHESQRDYAKGFGGQYGIQKDRVDKSAV
  GFNEMEAPTTAYKKTTPIEAASSGARGLKAKFESMAEEKRKREEEEKAQQVARRQQERKAVTKRSPEAPQ
  PVIAMEEPAVPAPLPKKISSEAWPPVGTPPSSESEPVRTSREHPVPLLPIRQTLPEDNEEPPALPPRTLE
  GLQVEEEPVYEAEPEPEPEPEPEPENDYEDVEEMDRHEQEDEPEGDYEEVLEPEDSSFSSALAGSSGCPA
  GAGAGAVALGISAVALYDYQGEGSDELSFDPDDVITDIEMVDEGWWRGRCHGHFGLFPANYVKLLE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005335
• ORF Size: 1458 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005335.3, NP_005326.1
• RefSeq Size: 2054 bp
• RefSeq ORF: 1461 bp
• Locus ID: 3059
• UniProt ID: P14317
• Cytogenetics: 3q13.33
• Domains: SH3, HS1_rep
• Protein Families: Transcription Factors
• Protein Pathways: Pathogenic Escherichia coli infection, Tight junction
• MW: 54 kDa
• Gene Summary: Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression.[UniProtKB/Swiss-Prot Function]