INSIG1 (NM_005542) Human Tagged ORF Clone

TrueORF Gold

Protein expression verified by Western blot, fully sequenced and in stock

Click here to learn more.

SKU

RC200312

INSIG1 (Myc-DDK-tagged)-Human insulin induced gene 1 (INSIG1), transcript variant 1

Datasheet SDS Plasmid Map

Reconstitution Protocol

NM_005542 in other vectors (6)

Be the first to review this product

$300.00

In Stock*

Bulk Requests & Clone Modifications

Frequently Bought Together (6)

TA345657	Rabbit Polyclonal Anti-INSIG1 Antibody	100 ul	$585.00
TA592569S	DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags	30 ul	$200.00
CC100003	DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)	5 x 200 ul	$160.00
PRIMER	Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each	100 pmol	$59.00
PS100001	pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug	10 ug	$650.00
AR100028M	DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL	5 ml	$720.00

Specifications

Specifications
Product Data
Type	Human Tagged ORF Clone
Target Symbol	INSIG1
Synonyms	CL6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence >RC200312 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCAGATTGCACGACCACTTCTGGAGCTGCTCCTGTGCGCACAGCGCGAGGCGCCGAGGCCCCCCGC GAGCCAGCGCCGCGGGGCTGGCGGCCAAGGTTGGGGAGATGATCAACGTTTCCGTGTCCGGGCCCTCCCT GCTGGCGGCCCACGGTGCCCCGGACGCTGACCCCGCGCCCAGGGGCCGCAGTGCTGCGATGAGCGGCCCC GAGCCCGGCAGCCCCTACCCCAACACCTGGCATCATCGCCTGTTGCAGAGGAGCCTCGTGCTCTTCTCGG TTGGGGTGGTCCTAGCCCTGGTGCTCAACCTGCTGCAGATCCAGAGGAATGTCACTCTCTTCCCCGAGGA GGTGATCGCCACCATCTTTTCCTCCGCCTGGTGGGTCCCTCCCTGCTGCGGGACAGCAGCTGCTGTTGTT GGCCTACTGTACCCCTGTATCGACAGTCACCTCGGAGAACCCCACAAATTTAAGAGAGAATGGGCCAGTG TCATGCGCTGCATAGCAGTTTTTGTTGGCATTAACCACGCCAGTGCTAAATTGGATTTTGCCAATAATGT CCAGCTGTCCTTGACTTTAGCAGCCCTATCTTTGGGCCTTTGGTGGACATTTGATCGTTCCAGAAGTGGC CTTGGGCTGGGGATCACCATAGCTTTTCTAGCTACGCTGATCACGCAGTTTCTCGTGTATAATGGTGTCT ATCAGTATACATCCCCAGATTTCCTCTATATTCGTTCTTGGCTCCCTTGTATATTTTTCTCAGGAGGCGT CACGGTGGGGAACATAGGACGACAGTTAGCTATGGGTGTTCCTGAAAAGCCCCATAGTGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence >RC200312 protein sequence Red=Cloning site Green=Tags(s) MPRLHDHFWSCSCAHSARRRGPPRASAAGLAAKVGEMINVSVSGPSLLAAHGAPDADPAPRGRSAAMSGP EPGSPYPNTWHHRLLQRSLVLFSVGVVLALVLNLLQIQRNVTLFPEEVIATIFSSAWWVPPCCGTAAAVV GLLYPCIDSHLGEPHKFKREWASVMRCIAVFVGINHASAKLDFANNVQLSLTLAALSLGLWWTFDRSRSG LGLGITIAFLATLITQFLVYNGVYQYTSPDFLYIRSWLPCIFFSGGVTVGNIGRQLAMGVPEKPHSD TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	Chromatograms Sequencher program is needed, download here
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_005542
ORF Size	831 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping	Ambient
Reference Data
RefSeq	NM_005542.6
RefSeq Size	3020 bp
RefSeq ORF	834 bp
Locus ID	3638
UniProt ID	O15503
Cytogenetics	7q36.3
Protein Families	Druggable Genome, Transmembrane
MW	30 kDa
Summary	This gene encodes an endoplasmic reticulum membrane protein that regulates cholesterol metabolism, lipogenesis, and glucose homeostasis. The encoded protein has six transmembrane helices which contain an effector protein binding site. It binds the sterol-sensing domains of sterol regulatory element-binding protein (SREBP) cleavage-activating protein (SCAP) and 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA reductase), and is essential for the sterol-mediated trafficking of these two proteins. It promotes the endoplasmic reticulum retention of SCAP and the ubiquitin-mediated degradation of HMG-CoA reductase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2016]

Reviews

Write Review

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System TrueClones: Full length cDNA clones (Human/Mouse/Rat), expressing native proteins pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
SDS for RC200312

Resources

cDNA Clone Resources
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones Protocol: Lentivirus packaging Product Sheet: AAV AAV Biosafety Lentivirus and AAV FAQ Virus-Mediated Gene Delivery

"NM_005542" in other vectors (6)

SKU	Description	Size	Price
RC200312L1	Lenti ORF clone of Human insulin induced gene 1 (INSIG1), transcript variant 1, Myc-DDK-tagged	10 ug	$600.00
RC200312L2	Lenti ORF clone of Human insulin induced gene 1 (INSIG1), transcript variant 1, mGFP tagged	10 ug	$600.00
RC200312L3	Lenti ORF clone of Human insulin induced gene 1 (INSIG1), transcript variant 1, Myc-DDK-tagged	10 ug	$600.00
RC200312L4	Lenti ORF clone of Human insulin induced gene 1 (INSIG1), transcript variant 1, mGFP tagged	10 ug	$600.00
RG200312	INSIG1 (tGFP-tagged) - Human insulin induced gene 1 (INSIG1), transcript variant 1	10 ug	$489.00 $500.00 $500.00
SC116677	INSIG1 (untagged)-Human insulin induced gene 1 (INSIG1), transcript variant 1	10 ug	$300.00

Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.