WDR1 (NM_017491) Human Tagged ORF Clone

CAT#: RC200303

WDR1 (Myc-DDK-tagged)-Human WD repeat domain 1 (WDR1), transcript variant 1

Product Images

Circular map for RC200303

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  Western blot validation of overexpression lysate (Cat# LY402592) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200303 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified WDR1 protein (Cat# TP300303). The protein was produced from HEK293T cells transfected with WDR1 cDNA clone (Cat# RC200303) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: WDR1
• Synonyms: AIP1; HEL-S-52; NORI-1; PFITS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200303 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGTACGAGATCAAGAAGGTGTTCGCCAGCCTCCCGCAGGTGGAGAGGGGCGTCTCCAAGATCATCG
  GCGGCGACCCTAAGGGCAACAATTTTCTGTACACCAATGGAAAGTGCGTCATCCTAAGGAACATCGACAA
  CCCAGCCCTTGCTGACATCTACACAGAGCACGCCCATCAGGTGGTGGTGGCCAAGTATGCGCCCAGCGGA
  TTCTACATTGCCTCCGGAGATGTGTCTGGGAAGCTGAGGATCTGGGATACCACGCAGAAGGAGCACCTGT
  TGAAGTATGAGTACCAGCCTTTCGCTGGGAAGATCAAAGACATTGCTTGGACTGAAGACAGTAAGAGGAT
  CGCCGTGGTCGGGGAAGGAAGGGAGAAGTTTGGAGCAGTCTTCCTCTGGGATAGTGGCTCTTCTGTGGGC
  GAGATTACAGGACACAACAAAGTCATCAACAGCGTGGACATCAAGCAGAGCCGGCCATACCGGCTGGCCA
  CGGGAAGCGATGATAACTGCGCGGCATTCTTTGAGGGACCCCCATTCAAGTTCAAGTTCACAATTGGCGA
  CCACAGCCGCTTTGTCAACTGTGTGCGATTCTCTCCTGATGGGAACAGATTTGCCACAGCCAGTGCTGAC
  GGCCAGATATACATCTATGACGGGAAGACTGGGGAGAAGGTGTGCGCGCTGGGCGGAAGCAAGGCCCACG
  ACGGTGGGATTTACGCAATTAGTTGGAGTCCCGACAGCACCCATTTGCTTTCTGCTTCTGGGGACAAAAC
  TTCCAAGATTTGGGACGTCAGCGTGAACTCCGTGGTCAGCACATTTCCCATGGGCTCCACGGTTCTGGAC
  CAGCAGCTGGGCTGCCTATGGCAGAAGGACCACCTGCTCAGTGTCTCCCTGTCCGGGTACATCAACTATC
  TGGACAGAAACAACCCCAGCAAGCCCCTGCACGTCATCAAGGGTCACAGTAAATCGATCCAGTGTCTGAC
  GGTGCATAAAAACGGCGGCAAGTCCTACATTTACTCTGGGAGCCACGACGGACACATTAATTACTGGGAT
  TCAGAGACGGGGGAGAACGACTCCTTCGCTGGGAAAGGCCACACGAACCAGGTGTCCAGGATGACCGTGG
  ATGAGTCGGGGCAGCTCATCAGCTGCAGCATGGACGACACCGTGCGGTACACCAGCCTCATGCTGCGGGA
  CTACAGCGGACAAGGAGTTGTGAAACTGGACGTTCAGCCAAAGTGCGTAGCCGTCGGCCCCGGGGGATAC
  GCCGTGGTCGTGTGCATTGGACAGATTGTCCTGCTGAAGGATCAGAGGAAGTGCTTCAGCATCGACAACC
  CCGGCTACGAGCCCGAAGTTGTGGCAGTGCACCCCGGCGGGGACACGGTGGCAATTGGGGGTGTGGACGG
  CAACGTCCGCCTGTATTCCATCCTGGGCACCACGTTGAAGGATGAGGGCAAGCTCCTAGAGGCCAAGGGC
  CCCGTGACCGACGTGGCCTACTCCCACGACGGCGCCTTCCTCGCGGTGTGCGACGCCAGCAAGGTGGTCA
  CAGTGTTCAGCGTTGCTGACGGCTACTCGGAGAACAATGTTTTTTATGGACACCATGCAAAAATCGTCTG
  CCTGGCCTGGTCCCCAGACAATGAACACTTTGCCTCCGGTGGCATGGACATGATGGTGTATGTTTGGACC
  CTGAGTGACCCGGAAACCAGAGTCAAGATCCAAGATGCACACCGGCTGCACCATGTCAGCAGCCTGGCCT
  GGCTGGACGAGCACACGCTGGTCACGACCTCCCATGATGCCTCTGTCAAGGAGTGGACAATCACCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200303 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPYEIKKVFASLPQVERGVSKIIGGDPKGNNFLYTNGKCVILRNIDNPALADIYTEHAHQVVVAKYAPSG
  FYIASGDVSGKLRIWDTTQKEHLLKYEYQPFAGKIKDIAWTEDSKRIAVVGEGREKFGAVFLWDSGSSVG
  EITGHNKVINSVDIKQSRPYRLATGSDDNCAAFFEGPPFKFKFTIGDHSRFVNCVRFSPDGNRFATASAD
  GQIYIYDGKTGEKVCALGGSKAHDGGIYAISWSPDSTHLLSASGDKTSKIWDVSVNSVVSTFPMGSTVLD
  QQLGCLWQKDHLLSVSLSGYINYLDRNNPSKPLHVIKGHSKSIQCLTVHKNGGKSYIYSGSHDGHINYWD
  SETGENDSFAGKGHTNQVSRMTVDESGQLISCSMDDTVRYTSLMLRDYSGQGVVKLDVQPKCVAVGPGGY
  AVVVCIGQIVLLKDQRKCFSIDNPGYEPEVVAVHPGGDTVAIGGVDGNVRLYSILGTTLKDEGKLLEAKG
  PVTDVAYSHDGAFLAVCDASKVVTVFSVADGYSENNVFYGHHAKIVCLAWSPDNEHFASGGMDMMVYVWT
  LSDPETRVKIQDAHRLHHVSSLAWLDEHTLVTTSHDASVKEWTITY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017491
• ORF Size: 1818 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017491.5
• RefSeq Size: 3160 bp
• RefSeq ORF: 1821 bp
• Locus ID: 9948
• UniProt ID: O75083
• Cytogenetics: 4p16.1
• Domains: WD40
• MW: 66.2 kDa
• Gene Summary: This gene encodes a protein containing 9 WD repeats. WD repeats are approximately 30- to 40-amino acid domains containing several conserved residues, mostly including a trp-asp at the C-terminal end. WD domains are involved in protein-protein interactions. The encoded protein may help induce the disassembly of actin filaments. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]