B4GALT7 (NM_007255) Human Tagged ORF Clone
B4GALT7 (Myc-DDK-tagged)-Human xylosylprotein beta 1,4-galactosyltransferase, polypeptide 7 (galactosyltransferase I) (B4GALT7)
|Product Name||B4GALT7 (NM_007255) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||EDSP1; EDSSLA; EDSSPD1; XGALT1; XGPT; XGPT1|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC200258 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC200258 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||981 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq||NM_007255.1, NM_007255.2, NP_009186.1|
|RefSeq Size||1747 bp|
|RefSeq ORF||984 bp|
|Protein Pathways||Chondroitin sulfate biosynthesis, Heparan sulfate biosynthesis, Metabolic pathways|
|Gene Summary||This gene is a member of the beta-1,4-galactosyltransferase (beta4GalT) family. Family members encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose. Each beta4GalT member has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus which then remains uncleaved to function as a transmembrane anchor. The enzyme encoded by this gene attaches the first galactose in the common carbohydrate-protein linkage (GlcA-beta1,3-Gal-beta1,3-Gal-beta1,4-Xyl-beta1-O-Ser) found in proteoglycans. This enzyme differs from other beta4GalTs because it lacks the conserved Cys residues found in beta4GalT1-beta4GalT6 and it is located in cis-Golgi instead of trans-Golgi. Mutations in this gene have been associated with the progeroid form of Ehlers-Danlos syndrome. [provided by RefSeq, Oct 2009]|
|The use of this cDNA Clones has been cited in the following citations:|
Expansion of B4GALT7 linkeropathy phenotype to include perinatal lethal skeletal dysplasia
,Mihalic Mosher, T;Zygmunt, DA;Koboldt, DC;Kelly, BJ;Johnson, LR;McKenna, DS;Hood, BC;Hickey, SE;White, P;Wilson, RK;Martin, PT;McBride, KL;,
Eur. J. Hum. Genet. 2019
,PubMed ID 31278392
The spatial separation of processing and transport functions to the interior and periphery of the Golgi stack
,Tie, HC;Ludwig, A;Sandin, S;Lu, L;,
,PubMed ID 30499774
|cDNA Clone Resources|
|SC319505||B4GALT7 (untagged)-Human xylosylprotein beta 1,4-galactosyltransferase, polypeptide 7 (galactosyltransferase I) (B4GALT7)||
|RG200258||B4GALT7 (GFP-tagged) - Human xylosylprotein beta 1,4-galactosyltransferase, polypeptide 7 (galactosyltransferase I) (B4GALT7)||
|RC200258L3||Lenti ORF clone of Human xylosylprotein beta 1,4-galactosyltransferase, polypeptide 7 (galactosyltransferase I) (B4GALT7), Myc-DDK-tagged||
|RC200258L4||Lenti ORF clone of Human xylosylprotein beta 1,4-galactosyltransferase, polypeptide 7 (galactosyltransferase I) (B4GALT7), mGFP tagged||