Flotillin 1 (FLOT1) (NM_005803) Human Tagged ORF Clone

CAT#: RC200231

Reviews ()
Write a review

FLOT1 (Myc-DDK-tagged)-Human flotillin 1 (FLOT1)



  "NM_005803" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 457.00

USD 686.00


Availability*
In Stock

Size
    • 10 ug


Product images

Other products for "FLOT1"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol FLOT1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC200231 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTTTTCACTTGTGGCCCAAATGAGGCCATGGTGGTCTCCGGGTTCTGCCGAAGCCCCCCAGTCATGG
TGGCTGGAGGGCGTGTCTTTGTCCTGCCCTGCATCCAACAGATCCAGAGGATCTCTCTCAACACACTGAC
CCTCAATGTCAAGAGTGAAAAGGTTTACACTCGCCATGGGGTCCCCATCTCAGTCACTGGCATTGCCCAG
GTAAAAATCCAGGGGCAGAACAAGGAGATGTTGGCGGCCGCCTGTCAGATGTTCCTGGGGAAGACGGAGG
CTGAGATTGCCCACATTGCCCTGGAGACGTTAGAGGGCCACCAGAGGGCCATCATGGCCCACATGACTGT
GGAGGAGATCTATAAGGACAGGCAGAAATTCTCAGAACAGGTTTTCAAAGTGGCCTCCTCAGACCTGGTC
AACATGGGCATCAGTGTGGTTAGCTACACTCTGAAGGACATTCACGATGACCAGGACTATTTGCACTCTT
TGGGGAAGGCTCGAACAGCTCAAGTCCAAAAAGATGCACGGATTGGAGAAGCAGAGGCCAAGAGAGATGC
TGGGATCCGGGAAGCTAAAGCCAAGCAGGAAAAGGTGTCTGCTCAGTACCTGAGTGAGATCGAGATGGCC
AAGGCACAGAGAGATTACGAACTGAAGAAGGCCGCCTATGACATCGAGGTCAACACCCGCCGAGCACAGG
CTGACCTGGCCTATCAGCTTCAGGTGGCCAAGACTAAGCAGCAGATTGAGGAGCAGCGGGTGCAGGTGCA
GGTGGTGGAGCGGGCCCAGCAGGTGGCAGTGCAGGAGCAGGAGATCGCCCGGCGGGAGAAGGAGCTGGAG
GCCCGGGTGCGGAAGCCAGCGGAAGCGGAGCGCTACAAGCTGGAGCGCCTAGCCGAGGCAGAGAAGTCCC
AACTAATTATGCAGGCGGAGGCAGAAGCCGCGTCTGTGCGGATGCGTGGGGAAGCTGAGGCCTTTGCCAT
AGGGGCCCGAGCCCGAGCCGAGGCTGAGCAGATGGCCAAGAAGGCAGAAGCCTTCCAGCTGTACCAAGAG
GCTGCTCAGCTGGACATGCTGCTAGAGAAGCTGCCCCAGGTGGCAGAGGAGATCAGTGGTCCCTTGACTT
CAGCCAATAAGATCACACTGGTGTCCAGCGGCAGTGGGACCATGGGGGCAGCCAAAGTGACTGGGGAAGT
ACTGGACATTCTAACTCGCCTGCCAGAGAGTGTGGAAAGACTCACAGGCGTGAGCATCTCCCAGGTGAAT
CACAAGCCTTTGAGAACAGCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC200231 protein sequence
Red=Cloning site Green=Tags(s)

MFFTCGPNEAMVVSGFCRSPPVMVAGGRVFVLPCIQQIQRISLNTLTLNVKSEKVYTRHGVPISVTGIAQ
VKIQGQNKEMLAAACQMFLGKTEAEIAHIALETLEGHQRAIMAHMTVEEIYKDRQKFSEQVFKVASSDLV
NMGISVVSYTLKDIHDDQDYLHSLGKARTAQVQKDARIGEAEAKRDAGIREAKAKQEKVSAQYLSEIEMA
KAQRDYELKKAAYDIEVNTRRAQADLAYQLQVAKTKQQIEEQRVQVQVVERAQQVAVQEQEIARREKELE
ARVRKPAEAERYKLERLAEAEKSQLIMQAEAEAASVRMRGEAEAFAIGARARAEAEQMAKKAEAFQLYQE
AAQLDMLLEKLPQVAEEISGPLTSANKITLVSSGSGTMGAAKVTGEVLDILTRLPESVERLTGVSISQVN
HKPLRTA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_005803
ORF Size 1281 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005803.4
RefSeq Size 1839 bp
RefSeq ORF 1284 bp
Locus ID 10211
UniProt ID O75955
Cytogenetics 6p21.33
Domains Band_7, Flotillin
Protein Pathways Insulin signaling pathway
MW 47.4 kDa
Gene Summary This gene encodes an protein that localizes to the caveolae, which are small domains on the inner cell membranes. This protein plays a role in vesicle trafficking and cell morphology. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]

Other Versions

Frequently bought together (5)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 30 ul

USD 177.00


pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

USD 650.00


Goat Polyclonal Antibody against Flotillin 1
    • 100 ug

USD 465.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

USD 160.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 59.00

Customer Reviews 
Loading...
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.