ACOX1 (NM_004035) Human Tagged ORF Clone

CAT#: RC200230

ACOX1 (Myc-DDK-tagged)-Human acyl-CoA oxidase 1, palmitoyl (ACOX1), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ACOX1
• Synonyms: ACOX; MITCH; PALMCOX; SCOX
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200230 representing NM_004035
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCCGGACCTGCGCAGGGAGCGGGATTCCGCCAGCTTCAACCCGGAGCTGCTTACACACATCCTGG
  ACGGCAGCCCCGAGAAAACCCGGCGCCGCCGAGAGATCGAGAACATGATCCTGAACGACCCAGACTTCCA
  GCATGAGGACTTGAACTTCCTCACTCGCAGCCAGCGTTATGAGGTGGCTGTCAGGAAAAGTGCCATCATG
  GTGAAGAAGATGAGGGAGTTTGGCATCGCTGACCCTGATGAAATTATGTGGTTTAAAAATTTTGTGCACC
  GAGGGCGGCCTGAGCCTCTGGATCTTCACTTGGGCATGTTCCTGCCCACCTTGCTTCACCAGGCAACTGC
  GGAGCAGCAGGAGCGCTTCTTCATGCCCGCCTGGAACTTGGAGATCATTGGCACTTATGCCCAGACAGAG
  ATGGGTCATGGAACTCACCTTCGAGGCTTGGAAACCACAGCCACGTATGACCCTGAAACCCAGGAGTTCA
  TTCTCAACAGTCCTACTGTGACCTCCATTAAATGGTGGCCTGGTGGGCTTGGAAAGACTTCAAATCATGC
  AATAGTTCTTGCCCAGCTCATCACTAAGGGGAAATGCTATGGATTACATGCCTTTATCGTACCTATTCGT
  GAAATCGGGACCCATAAGCCTTTGCCAGGAATTACCGTTGGTGACATCGGCCCCAAATTTGGTTATGATG
  AGATAGACAATGGCTACCTCAAAATGGACAACCATCGTATTCCCAGAGAAAACATGCTGATGAAGTATGC
  CCAGGTGAAGCCTGATGGCACATACGTGAAACCGCTGAGTAACAAGCTGACTTACGGGACCATGGTGTTT
  GTCAGGTCCTTCCTTGTGGGAGAAGCTGCTCGGGCTCTGTCTAAGGCGTGCACCATTGCCATCCGATACA
  GCGCTGTGAGGCACCAGTCTGAAATCAAGCCAGGTGAACCAGAACCACAGATTTTGGATTTTCAAACCCA
  GCAGTATAAACTCTTTCCACTCCTGGCCACTGCCTATGCCTTCCAGTTTGTGGGCGCATACATGAAGGAG
  ACCTATCACCGGATTAACGAAGGCATTGGTCAAGGGGACCTGAGTGAACTGCCTGAGCTTCATGCCCTCA
  CCGCTGGACTGAAGGCTTTCACCTCCTGGACTGCAAACACTGGCATTGAAGCATGTCGGATGGCTTGTGG
  TGGGCATGGCTATTCTCATTGCAGTGGTCTTCCAAATATTTATGTCAATTTCACCCCAAGCTGTACCTTT
  GAGGGAGAAAACACTGTCATGATGCTCCAGACGGCTAGGTTCCTGATGAAAAGTTATGATCAGGTGCACT
  CAGGAAAGTTGGTGTGTGGCATGGTGTCCTATTTGAACGACCTGCCCAGTCAGCGCATCCAGCCACAGCA
  GGTAGCAGTCTGGCCAACCATGGTGGATATCAACAGCCCCGAAAGCCTAACCGAAGCATATAAACTCCGT
  GCAGCCAGATTAGTAGAAATTGCTGCAAAAAACCTTCAAAAAGAAGTGATTCACAGAAAAAGCAAGGAGG
  TAGCTTGGAACCTAACTTCTGTTGACCTTGTTCGAGCAAGTGAGGCACATTGCCACTATGTGGTAGTTAA
  GCTCTTTTCAGAAAAACTCCTCAAAATTCAAGATAAAGCCATTCAAGCTGTCTTAAGGAGTTTATGTCTG
  CTGTATTCTCTGTATGGAATCAGTCAGAACGCGGGGGATTTCCTTCAGGGGAGCATCATGACAGAGCCTC
  AGATTACACAAGTAAACCAGCGTGTAAAGGAGTTACTCACTCTGATTCGCTCAGATGCTGTTGCTTTGGT
  TGATGCATTTGATTTTCAGGATGTGACACTTGGCTCTGTGCTTGGCCGCTATGATGGGAATGTGTATGAA
  AACTTGTTTGAGTGGGCTAAGAACTCCCCACTGAACAAAGCAGAGGTCCACGAATCTTACAAGCACCTGA
  AGTCACTGCAGTCCAAGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200230 representing NM_004035
  Red=Cloning site Green=Tags(s)
  
  MNPDLRRERDSASFNPELLTHILDGSPEKTRRRREIENMILNDPDFQHEDLNFLTRSQRYEVAVRKSAIM
  VKKMREFGIADPDEIMWFKNFVHRGRPEPLDLHLGMFLPTLLHQATAEQQERFFMPAWNLEIIGTYAQTE
  MGHGTHLRGLETTATYDPETQEFILNSPTVTSIKWWPGGLGKTSNHAIVLAQLITKGKCYGLHAFIVPIR
  EIGTHKPLPGITVGDIGPKFGYDEIDNGYLKMDNHRIPRENMLMKYAQVKPDGTYVKPLSNKLTYGTMVF
  VRSFLVGEAARALSKACTIAIRYSAVRHQSEIKPGEPEPQILDFQTQQYKLFPLLATAYAFQFVGAYMKE
  TYHRINEGIGQGDLSELPELHALTAGLKAFTSWTANTGIEACRMACGGHGYSHCSGLPNIYVNFTPSCTF
  EGENTVMMLQTARFLMKSYDQVHSGKLVCGMVSYLNDLPSQRIQPQQVAVWPTMVDINSPESLTEAYKLR
  AARLVEIAAKNLQKEVIHRKSKEVAWNLTSVDLVRASEAHCHYVVVKLFSEKLLKIQDKAIQAVLRSLCL
  LYSLYGISQNAGDFLQGSIMTEPQITQVNQRVKELLTLIRSDAVALVDAFDFQDVTLGSVLGRYDGNVYE
  NLFEWAKNSPLNKAEVHESYKHLKSLQSKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004035
• ORF Size: 1980 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004035.7
• RefSeq Size: 7585 bp
• RefSeq ORF: 1983 bp
• Locus ID: 51
• UniProt ID: Q15067
• Cytogenetics: 17q25.1
• Domains: ACOX, Acyl-CoA_dh
• Protein Families: Druggable Genome
• Protein Pathways: alpha-Linolenic acid metabolism, Biosynthesis of unsaturated fatty acids, Fatty acid metabolism, Metabolic pathways, PPAR signaling pathway
• MW: 74.7 kDa
• Gene Summary: The protein encoded by this gene is the first enzyme of the fatty acid beta-oxidation pathway, which catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. It donates electrons directly to molecular oxygen, thereby producing hydrogen peroxide. Defects in this gene result in pseudoneonatal adrenoleukodystrophy, a disease that is characterized by accumulation of very long chain fatty acids. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]