FAIM2 (NM_012306) Human Tagged ORF Clone

CAT#: RC200196

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FAIM2 (Myc-DDK-tagged)-Human Fas apoptotic inhibitory molecule 2 (FAIM2)



  "NM_012306" in other vectors (6)

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 118.00

USD 517.00


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Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol FAIM2
Synonyms LFG; LFG2; NGP35; NMP35; TMBIM2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC200196 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCCAGGGAAAGCTCTCCGTGGCTAACAAGGCCCCTGGGACCGAGGGGCAGCAGCAGGTGCATGGCG
AGAAGAAGGAGGCTCCAGCAGTGCCCTCAGCCCCACCCTCCTATGAGGAAGCCACCTCTGGGGAGGGGAT
GAAGGCAGGGGCCTTCCCCCCAGCCCCCACAGCGGTGCCTCTCCACCCTAGCTGGGCCTATGTGGACCCC
AGCAGCAGCTCCAGCTATGACAACGGTTTCCCCACCGGAGACCATGAGCTCTTCACCACTTTCAGCTGGG
ATGACCAGAAAGTTCGTCGAGTCTTTGTCAGAAAGGTCTACACCATCCTGCTGATTCAGCTGCTGGTGAC
CTTGGCTGTCGTGGCTCTCTTTACTTTCTGTGACCCTGTCAAGGACTATGTCCAGGCCAACCCAGGCTGG
TACTGGGCATCCTATGCTGTGTTCTTTGCAACCTACCTGACCCTGGCTTGCTGTTCTGGACCCAGGAGGC
ATTTCCCCTGGAACCTGATTCTCCTGACCGTCTTTACCCTGTCCATGGCCTACCTCACTGGGATGCTGTC
CAGCTACTACAACACCACCTCCGTGCTGCTGTGCCTGGGCATCACGGCCCTTGTCTGCCTCTCAGTCACC
GTCTTCAGCTTCCAGACCAAGTTCGACTTCACCTCCTGCCAGGGCGTGCTCTTCGTGCTTCTCATGACTC
TTTTCTTCAGCGGACTCATCCTGGCCATCCTCCTACCCTTCCAATATGTGCCCTGGCTCCATGCAGTTTA
TGCAGCACTGGGAGCGGGTGTATTTACATTGTTCCTGGCACTTGACACCCAGTTGCTGATGGGTAACCGA
CGCCACTCGCTGAGCCCTGAGGAGTATATTTTTGGAGCCCTCAACATTTACCTAGACATCATCTATATCT
TCACCTTCTTCCTGCAGCTTTTTGGCACTAACCGAGAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC200196 protein sequence
Red=Cloning site Green=Tags(s)

MTQGKLSVANKAPGTEGQQQVHGEKKEAPAVPSAPPSYEEATSGEGMKAGAFPPAPTAVPLHPSWAYVDP
SSSSSYDNGFPTGDHELFTTFSWDDQKVRRVFVRKVYTILLIQLLVTLAVVALFTFCDPVKDYVQANPGW
YWASYAVFFATYLTLACCSGPRRHFPWNLILLTVFTLSMAYLTGMLSSYYNTTSVLLCLGITALVCLSVT
VFSFQTKFDFTSCQGVLFVLLMTLFFSGLILAILLPFQYVPWLHAVYAALGAGVFTLFLALDTQLLMGNR
RHSLSPEEYIFGALNIYLDIIYIFTFFLQLFGTNRE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_012306
ORF Size 948 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_012306.4
RefSeq Size 4744 bp
RefSeq ORF 951 bp
Locus ID 23017
UniProt ID Q9BWQ8
Cytogenetics 12q13.12
Domains UPF0005
Protein Families Transmembrane
MW 35.1 kDa
Gene Summary Antiapoptotic protein which protects cells uniquely from Fas-induced apoptosis. Regulates Fas-mediated apoptosis in neurons by interfering with caspase-8 activation. May play a role in cerebellar development by affecting cerebellar size, internal granular layer (IGL) thickness, and Purkinje cell (PC) development.[UniProtKB/Swiss-Prot Function]

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