FAIM2 (NM_012306) Human Tagged ORF Clone

CAT#: RC200196

FAIM2 (Myc-DDK-tagged)-Human Fas apoptotic inhibitory molecule 2 (FAIM2)

Product Images

Western blot validation of overexpression lysate (Cat# LY415867) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200196 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified FAIM2 protein (Cat# TP300196). The protein was produced from HEK293T cells transfected with FAIM2 cDNA clone (Cat# RC200196) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FAIM2
• Synonyms: LFG; LFG2; NGP35; NMP35; TMBIM2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200196 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCCAGGGAAAGCTCTCCGTGGCTAACAAGGCCCCTGGGACCGAGGGGCAGCAGCAGGTGCATGGCG
  AGAAGAAGGAGGCTCCAGCAGTGCCCTCAGCCCCACCCTCCTATGAGGAAGCCACCTCTGGGGAGGGGAT
  GAAGGCAGGGGCCTTCCCCCCAGCCCCCACAGCGGTGCCTCTCCACCCTAGCTGGGCCTATGTGGACCCC
  AGCAGCAGCTCCAGCTATGACAACGGTTTCCCCACCGGAGACCATGAGCTCTTCACCACTTTCAGCTGGG
  ATGACCAGAAAGTTCGTCGAGTCTTTGTCAGAAAGGTCTACACCATCCTGCTGATTCAGCTGCTGGTGAC
  CTTGGCTGTCGTGGCTCTCTTTACTTTCTGTGACCCTGTCAAGGACTATGTCCAGGCCAACCCAGGCTGG
  TACTGGGCATCCTATGCTGTGTTCTTTGCAACCTACCTGACCCTGGCTTGCTGTTCTGGACCCAGGAGGC
  ATTTCCCCTGGAACCTGATTCTCCTGACCGTCTTTACCCTGTCCATGGCCTACCTCACTGGGATGCTGTC
  CAGCTACTACAACACCACCTCCGTGCTGCTGTGCCTGGGCATCACGGCCCTTGTCTGCCTCTCAGTCACC
  GTCTTCAGCTTCCAGACCAAGTTCGACTTCACCTCCTGCCAGGGCGTGCTCTTCGTGCTTCTCATGACTC
  TTTTCTTCAGCGGACTCATCCTGGCCATCCTCCTACCCTTCCAATATGTGCCCTGGCTCCATGCAGTTTA
  TGCAGCACTGGGAGCGGGTGTATTTACATTGTTCCTGGCACTTGACACCCAGTTGCTGATGGGTAACCGA
  CGCCACTCGCTGAGCCCTGAGGAGTATATTTTTGGAGCCCTCAACATTTACCTAGACATCATCTATATCT
  TCACCTTCTTCCTGCAGCTTTTTGGCACTAACCGAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200196 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTQGKLSVANKAPGTEGQQQVHGEKKEAPAVPSAPPSYEEATSGEGMKAGAFPPAPTAVPLHPSWAYVDP
  SSSSSYDNGFPTGDHELFTTFSWDDQKVRRVFVRKVYTILLIQLLVTLAVVALFTFCDPVKDYVQANPGW
  YWASYAVFFATYLTLACCSGPRRHFPWNLILLTVFTLSMAYLTGMLSSYYNTTSVLLCLGITALVCLSVT
  VFSFQTKFDFTSCQGVLFVLLMTLFFSGLILAILLPFQYVPWLHAVYAALGAGVFTLFLALDTQLLMGNR
  RHSLSPEEYIFGALNIYLDIIYIFTFFLQLFGTNRE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012306
• ORF Size: 948 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012306.4
• RefSeq Size: 4744 bp
• RefSeq ORF: 951 bp
• Locus ID: 23017
• UniProt ID: Q9BWQ8
• Cytogenetics: 12q13.12
• Domains: UPF0005
• Protein Families: Transmembrane
• MW: 35.1 kDa
• Gene Summary: Antiapoptotic protein which protects cells uniquely from Fas-induced apoptosis. Regulates Fas-mediated apoptosis in neurons by interfering with caspase-8 activation. May play a role in cerebellar development by affecting cerebellar size, internal granular layer (IGL) thickness, and Purkinje cell (PC) development.[UniProtKB/Swiss-Prot Function]