MRPL16 (NM_017840) Human Tagged ORF Clone
CAT#: RC200167
MRPL16 (Myc-DDK-tagged)-Human mitochondrial ribosomal protein L16 (MRPL16), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_017840" in other vectors (5)
USD 198.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | MRPL16 |
Synonyms | L16mt; MRP-L16; PNAS-111 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC200167 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGAGGCTGCTGGCTCGCGCTAGTGCGCCGCTCCTGCGGGTGCCCTTGTCAGATTCCTGGGCACTCC TCCCCGCCAGTGCTGGCGTAAAGACACTGCTCCCAGTACCAAGTTTTGAAGATGTTTCCATTCCTGAAAA ACCCAAGCTTAGATTTATTGAAAGGGCACCACTTGTGCCAAAAGTAAGAAGAGAACCTAAAAATTTAAGT GACATACGGGGACCTTCCACTGAAGCTACGGAGTTTACAGAAGGCAATTTTGCAATCTTGGCATTGGGTG GTGGCTACCTGCATTGGGGCCACTTTGAAATGATGCGCCTGACAATCAACCGCTCTATGGACCCCAAGAA CATGTTTGCCATATGGCGAGTACCAGCCCCTTTCAAGCCCATCACTCGCAAAAGTGTTGGGCATCGCATG GGGGGAGGCAAAGGTGCTATTGACCACTACGTGACACCTGTGAAGGCTGGCCGCCTTGTTGTAGAGATGG GTGGGCGTTGTGAATTTGAAGAAGTGCAAGGTTTCCTTGACCAGGTTGCCCACAAGTTGCCCTTCGCAGC AAAGGCTGTGAGCCGCGGGACTCTAGAGAAGATGCGAAAAGATCAAGAGGAAAGAGAACGTAACAACCAG AACCCCTGGACATTTGAGCGAATAGCCACTGCCAACATGCTGGGCATACGGAAAGTACTGAGCCCATATG ACTTGACCCACAAGGGGAAATACTGGGGCAAGTTCTACATGCCCAAACGTGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200167 protein sequence
Red=Cloning site Green=Tags(s) MWRLLARASAPLLRVPLSDSWALLPASAGVKTLLPVPSFEDVSIPEKPKLRFIERAPLVPKVRREPKNLS DIRGPSTEATEFTEGNFAILALGGGYLHWGHFEMMRLTINRSMDPKNMFAIWRVPAPFKPITRKSVGHRM GGGKGAIDHYVTPVKAGRLVVEMGGRCEFEEVQGFLDQVAHKLPFAAKAVSRGTLEKMRKDQEERERNNQ NPWTFERIATANMLGIRKVLSPYDLTHKGKYWGKFYMPKRV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_017840 |
ORF Size | 753 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_017840.4 |
RefSeq Size | 1196 bp |
RefSeq ORF | 756 bp |
Locus ID | 54948 |
UniProt ID | Q9NX20 |
Cytogenetics | 11q12.1 |
Domains | Ribosomal_L16 |
MW | 28.4 kDa |
Gene Summary | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC200167L3 | Lenti ORF clone of Human mitochondrial ribosomal protein L16 (MRPL16), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
USD 600.00 |
|
RC200167L4 | Lenti ORF clone of Human mitochondrial ribosomal protein L16 (MRPL16), nuclear gene encoding mitochondrial protein, mGFP tagged |
USD 600.00 |
|
RG200167 | MRPL16 (tGFP-tagged) - Human mitochondrial ribosomal protein L16 (MRPL16), nuclear gene encoding mitochondrial protein |
USD 500.00 |
|
SC113890 | MRPL16 (untagged)-Human mitochondrial ribosomal protein L16 (MRPL16), nuclear gene encoding mitochondrial protein |
USD 300.00 |
|
SC320577 | MRPL16 (untagged)-Human mitochondrial ribosomal protein L16 (MRPL16), nuclear gene encoding mitochondrial protein |
USD 300.00 |
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