GGA2 (NM_015044) Human Tagged ORF Clone

CAT#: RC200153

GGA2 (Myc-DDK-tagged)-Human golgi-associated, gamma adaptin ear containing, ARF binding protein 2 (GGA2)

Product Images

Circular map for RC200153

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GGA2 (Cat# RC200153, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GGA2(Cat# TA507282). Positive lysates LY414846 (100ug) and LC414846 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY414846) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200153 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified GGA2 protein (Cat# TP300153). The protein was produced from HEK293T cells transfected with GGA2 cDNA clone (Cat# RC200153) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GGA2
• Synonyms: VEAR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200153 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGACCGCGGTGGCGGCGGCTGTGGCGGGAACCGAGTCGGCCCAGGGTCCCCCGGGCCCGGCAG
  CGTCGCTGGAGCTGTGGCTGAACAAAGCCACAGACCCAAGCATGTCGGAACAGGATTGGTCAGCTATCCA
  GAATTTCTGTGAGCAGGTGAACACTGACCCCAATGGCCCCACACATGCGCCCTGGCTACTGGCCCACAAG
  ATCCAGTCTCCGCAAGAGAAGGAAGCTCTTTATGCCTTAACGGTGCTGGAGATGTGCATGAACCACTGTG
  GGGAGAAGTTCCACAGCGAGGTGGCCAAATTTCGTTTCCTGAACGAACTGATCAAAGTGTTGTCCCCAAA
  GTACCTGGGGTCCTGGGCCACAGGAAAAGTTAAAGGAAGAGTCATTGAAATACTCTTCAGTTGGACAGTC
  TGGTTTCCGGAAGACATCAAGATTCGAGACGCTTATCAGATGCTGAAGAAACAAGGAATTATAAAACAAG
  ACCCTAAACTACCAGTGGATAAAATCTTACCCCCACCATCTCCCTGGCCCAAGAGCTCCATCTTTGATGC
  TGATGAAGAAAAGTCCAAGCTTCTGACAAGGCTTCTAAAGAGCAACCACCCCGAGGACCTTCAGGCTGCA
  AACCGGTTAATCAAGAATTTGGTCAAGGAGGAACAAGAAAAATCGGAGAAGGTGTCCAAGAGGGTCAGTG
  CGGTGGAGGAAGTGCGAAGCCATGTGAAGGTGCTGCAGGAGATGCTGAGCATGTACCGCAGGCCAGGGCA
  GGCCCCGCCCGACCAGGAGGCCCTGCAGGTCGTGTATGAGAGGTGTGAAAAGCTGCGGCCCACGCTGTTC
  CGGTTGGCGAGTGACACCACTGATGACGATGATGCACTCGCGGAAATTCTCCAGGCAAATGACCTCCTCA
  CCCAAGGAGTTCTGCTGTACAAACAGGTGATGGAGGGCCGGGTCACCTTTGGAAACAGAGTGACCAGCTC
  ATTGGGAGACATCCCTGTCTCCAGAGTCTTTCAGAATCCAGCAGGCTGCATGAAGACCTGCCCCCTGATT
  GACTTGGAGGTGGACAATGGACCTGCGCAGATGGGGACTGTGGTGCCATCTTTGCTTCATCAGGACCTGG
  CAGCCTTGGGAATCAGTGATGCTCCTGTTACAGGCATGGTTTCTGGTCAGAATTGCTGTGAGGAAAAGAG
  GAATCCCTCCTCCAGCACGCTGCCAGGCGGTGGTGTTCAGAACCCTTCTGCAGACAGGAATTTGCTGGAC
  CTCCTCTCACCACAGCCAGCTCCGTGCCCTCTGAATTATGTTTCGCAGAAAAGTGTCCCCAAGGAAGTGC
  CACCAGGTACTAAGTCCTCTCCAGGTTGGTCCTGGGAGGCTGGCCCGTTGGCTCCTTCCCCATCTTCACA
  GAATACACCTCTGGCTCAAGTGTTTGTCCCTTTGGAGTCTGTTAAGCCCAGCAGCCTGCCGCCTCTCATT
  GTGTATGACCGGAATGGATTCAGAATTCTGCTCCACTTCTCCCAGACGGGAGCCCCTGGGCACCCAGAGG
  TACAGGTGCTGCTCTTGACCATGATGAGCACGGCTCCCCAGCCTGTCTGGGATATCATGTTTCAAGTGGC
  TGTGCCAAAGTCAATGAGAGTGAAGCTGCAGCCGGCATCCAGCTCCAAGCTTCCTGCATTCAGTCCTTTG
  ATGCCTCCAGCTGTGATATCTCAGATGCTGCTGCTTGACAATCCACACAAAGAACCTATCCGCTTACGGT
  ACAAGCTGACATTCAACCAAGGTGGACAGCCTTTCAGCGAAGTAGGAGAAGTGAAAGACTTCCCAGACCT
  GGCTGTCTTGGGCGCAGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200153 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAATAVAAAVAGTESAQGPPGPAASLELWLNKATDPSMSEQDWSAIQNFCEQVNTDPNGPTHAPWLLAHK
  IQSPQEKEALYALTVLEMCMNHCGEKFHSEVAKFRFLNELIKVLSPKYLGSWATGKVKGRVIEILFSWTV
  WFPEDIKIRDAYQMLKKQGIIKQDPKLPVDKILPPPSPWPKSSIFDADEEKSKLLTRLLKSNHPEDLQAA
  NRLIKNLVKEEQEKSEKVSKRVSAVEEVRSHVKVLQEMLSMYRRPGQAPPDQEALQVVYERCEKLRPTLF
  RLASDTTDDDDALAEILQANDLLTQGVLLYKQVMEGRVTFGNRVTSSLGDIPVSRVFQNPAGCMKTCPLI
  DLEVDNGPAQMGTVVPSLLHQDLAALGISDAPVTGMVSGQNCCEEKRNPSSSTLPGGGVQNPSADRNLLD
  LLSPQPAPCPLNYVSQKSVPKEVPPGTKSSPGWSWEAGPLAPSPSSQNTPLAQVFVPLESVKPSSLPPLI
  VYDRNGFRILLHFSQTGAPGHPEVQVLLLTMMSTAPQPVWDIMFQVAVPKSMRVKLQPASSSKLPAFSPL
  MPPAVISQMLLLDNPHKEPIRLRYKLTFNQGGQPFSEVGEVKDFPDLAVLGAA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015044
• ORF Size: 1839 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_015044.4
• RefSeq Size: 5973 bp
• RefSeq ORF: 1842 bp
• Locus ID: 23062
• UniProt ID: Q9UJY4
• Cytogenetics: 16p12.2
• Domains: VHS, GAT, Alpha_adaptinC2
• Protein Pathways: Lysosome
• MW: 67.2 kDa
• Gene Summary: This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) family. This family includes ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. This family member may play a significant role in cargo molecules regulation and clathrin-coated vesicle assembly. [provided by RefSeq, Jul 2008]