GGA2 (NM_015044) Human Tagged ORF Clone

CAT#: RC200153

Reviews ()
Write a review

GGA2 (Myc-DDK-tagged)-Human golgi-associated, gamma adaptin ear containing, ARF binding protein 2 (GGA2)



Reconstitution Protocol

USD 571.00

USD 856.00

In Stock*

Size
    • 10 ug

Product images

Frequently bought together (4)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 30 ul

USD 177.00


pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

USD 650.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

USD 160.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 59.00

Other products for "GGA2"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol GGA2
Synonyms VEAR
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC200153 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGACCGCGGTGGCGGCGGCTGTGGCGGGAACCGAGTCGGCCCAGGGTCCCCCGGGCCCGGCAG
CGTCGCTGGAGCTGTGGCTGAACAAAGCCACAGACCCAAGCATGTCGGAACAGGATTGGTCAGCTATCCA
GAATTTCTGTGAGCAGGTGAACACTGACCCCAATGGCCCCACACATGCGCCCTGGCTACTGGCCCACAAG
ATCCAGTCTCCGCAAGAGAAGGAAGCTCTTTATGCCTTAACGGTGCTGGAGATGTGCATGAACCACTGTG
GGGAGAAGTTCCACAGCGAGGTGGCCAAATTTCGTTTCCTGAACGAACTGATCAAAGTGTTGTCCCCAAA
GTACCTGGGGTCCTGGGCCACAGGAAAAGTTAAAGGAAGAGTCATTGAAATACTCTTCAGTTGGACAGTC
TGGTTTCCGGAAGACATCAAGATTCGAGACGCTTATCAGATGCTGAAGAAACAAGGAATTATAAAACAAG
ACCCTAAACTACCAGTGGATAAAATCTTACCCCCACCATCTCCCTGGCCCAAGAGCTCCATCTTTGATGC
TGATGAAGAAAAGTCCAAGCTTCTGACAAGGCTTCTAAAGAGCAACCACCCCGAGGACCTTCAGGCTGCA
AACCGGTTAATCAAGAATTTGGTCAAGGAGGAACAAGAAAAATCGGAGAAGGTGTCCAAGAGGGTCAGTG
CGGTGGAGGAAGTGCGAAGCCATGTGAAGGTGCTGCAGGAGATGCTGAGCATGTACCGCAGGCCAGGGCA
GGCCCCGCCCGACCAGGAGGCCCTGCAGGTCGTGTATGAGAGGTGTGAAAAGCTGCGGCCCACGCTGTTC
CGGTTGGCGAGTGACACCACTGATGACGATGATGCACTCGCGGAAATTCTCCAGGCAAATGACCTCCTCA
CCCAAGGAGTTCTGCTGTACAAACAGGTGATGGAGGGCCGGGTCACCTTTGGAAACAGAGTGACCAGCTC
ATTGGGAGACATCCCTGTCTCCAGAGTCTTTCAGAATCCAGCAGGCTGCATGAAGACCTGCCCCCTGATT
GACTTGGAGGTGGACAATGGACCTGCGCAGATGGGGACTGTGGTGCCATCTTTGCTTCATCAGGACCTGG
CAGCCTTGGGAATCAGTGATGCTCCTGTTACAGGCATGGTTTCTGGTCAGAATTGCTGTGAGGAAAAGAG
GAATCCCTCCTCCAGCACGCTGCCAGGCGGTGGTGTTCAGAACCCTTCTGCAGACAGGAATTTGCTGGAC
CTCCTCTCACCACAGCCAGCTCCGTGCCCTCTGAATTATGTTTCGCAGAAAAGTGTCCCCAAGGAAGTGC
CACCAGGTACTAAGTCCTCTCCAGGTTGGTCCTGGGAGGCTGGCCCGTTGGCTCCTTCCCCATCTTCACA
GAATACACCTCTGGCTCAAGTGTTTGTCCCTTTGGAGTCTGTTAAGCCCAGCAGCCTGCCGCCTCTCATT
GTGTATGACCGGAATGGATTCAGAATTCTGCTCCACTTCTCCCAGACGGGAGCCCCTGGGCACCCAGAGG
TACAGGTGCTGCTCTTGACCATGATGAGCACGGCTCCCCAGCCTGTCTGGGATATCATGTTTCAAGTGGC
TGTGCCAAAGTCAATGAGAGTGAAGCTGCAGCCGGCATCCAGCTCCAAGCTTCCTGCATTCAGTCCTTTG
ATGCCTCCAGCTGTGATATCTCAGATGCTGCTGCTTGACAATCCACACAAAGAACCTATCCGCTTACGGT
ACAAGCTGACATTCAACCAAGGTGGACAGCCTTTCAGCGAAGTAGGAGAAGTGAAAGACTTCCCAGACCT
GGCTGTCTTGGGCGCAGCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC200153 protein sequence
Red=Cloning site Green=Tags(s)

MAATAVAAAVAGTESAQGPPGPAASLELWLNKATDPSMSEQDWSAIQNFCEQVNTDPNGPTHAPWLLAHK
IQSPQEKEALYALTVLEMCMNHCGEKFHSEVAKFRFLNELIKVLSPKYLGSWATGKVKGRVIEILFSWTV
WFPEDIKIRDAYQMLKKQGIIKQDPKLPVDKILPPPSPWPKSSIFDADEEKSKLLTRLLKSNHPEDLQAA
NRLIKNLVKEEQEKSEKVSKRVSAVEEVRSHVKVLQEMLSMYRRPGQAPPDQEALQVVYERCEKLRPTLF
RLASDTTDDDDALAEILQANDLLTQGVLLYKQVMEGRVTFGNRVTSSLGDIPVSRVFQNPAGCMKTCPLI
DLEVDNGPAQMGTVVPSLLHQDLAALGISDAPVTGMVSGQNCCEEKRNPSSSTLPGGGVQNPSADRNLLD
LLSPQPAPCPLNYVSQKSVPKEVPPGTKSSPGWSWEAGPLAPSPSSQNTPLAQVFVPLESVKPSSLPPLI
VYDRNGFRILLHFSQTGAPGHPEVQVLLLTMMSTAPQPVWDIMFQVAVPKSMRVKLQPASSSKLPAFSPL
MPPAVISQMLLLDNPHKEPIRLRYKLTFNQGGQPFSEVGEVKDFPDLAVLGAA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_015044
ORF Size 1839 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_015044.4
RefSeq Size 5973 bp
RefSeq ORF 1842 bp
Locus ID 23062
UniProt ID Q9UJY4
Cytogenetics 16p12.2
Domains VHS, GAT, Alpha_adaptinC2
Protein Pathways Lysosome
MW 67.2 kDa
Gene Summary This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) family. This family includes ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. This family member may play a significant role in cargo molecules regulation and clathrin-coated vesicle assembly. [provided by RefSeq, Jul 2008]
Customer Reviews 
Loading...
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.