REC8 (NM_005132) Human Tagged ORF Clone

CAT#: RC200132

REC8 (Myc-DDK-tagged)-Human REC8 homolog (yeast) (REC8), transcript variant 1

Product Images

Circular map for RC200132

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  Western blot validation of overexpression lysate (Cat# LY401575) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200132 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified REC8 protein (Cat# TP300132). The protein was produced from HEK293T cells transfected with REC8 cDNA clone (Cat# RC200132) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: REC8
• Synonyms: HR21spB; REC8L1; Rec8p
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200132 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCTACTATCCCAACGTGCTTCAGCGCCACACCGGCTGCTTTGCCACCATCTGGCTGGCGGCGACTC
  GCGGCAGCCGGTTGGTGAAGCGCGAATACCTGAGGGTGAATGTGGTGAAAACCTGCGAGGAAATCCTCAA
  TTACGTGCTGGTACGAGTGCAACCCCCGCAGCCCGGCCTGCCGCGGCCCCGCTTCTCCCTCTATCTCTCA
  GCCCAACTTCAGATCGGTGTGATCCGCGTCTATTCTCAACAATGCCAGTACCTCGTGGAGGACATCCAGC
  ACATCTTGGAGCGCCTCCACCGTGCCCAGCTGCAGATCCGAATAGATATGGAGACTGAGCTACCCAGCCT
  GCTGCTTCCTAACCACCTGGCCATGATGGAGACCCTAGAAGATGCTCCAGATCCCTTTTTTGGGATGATG
  TCTGTGGATCCCAGACTTCCTAGTCCTTTCGATATCCCTCAGATTCGACACCTCTTAGAGGCTGCAATCC
  CAGAGAGAGTTGAAGAGATCCCTCCTGAAGTTCCTACAGAGCCCAGGGAGCCAGAGAGGATTCCGGTCAC
  TGTGCTGCCACCTGAGGCCATCACGATCCTGGAGGCAGAGCCCATACGGATGCTGGAGATTGAGGGTGAA
  CGGGAGCTCCCAGAGGTCAGCCGCCGAGAACTGGACCTGCTGATCGCAGAGGAAGAAGAAGCTATCTTGT
  TAGAAATCCCGCGGCTCCCACCTCCAGCTCCTGCAGAGGTGGAAGGAATAGGAGAGGCACTGGGTCCTGA
  GGAGCTGAGGCTGACAGGCTGGGAACCTGGGGCCCTACTCATGGAGGTGACCCCCCCGGAGGAGCTGCGT
  CTGCCAGCCCCACCCAGCCCAGAGAGGAGGCCCCCAGTCCCCCCACCTCCTCGCCGCCGCCGTCGTCGCC
  GGTTACTGTTCTGGGACAAGGAGACTCAGATCTCCCCGGAGAAATTCCAGGAACAACTGCAAACCAGAGC
  CCACTGCTGGGAATGTCCTATGGTGCAGCCGCCCGAGAGGACCATCAGAGGCCCTGCGGAGTTGTTCAGA
  ACCCCAACTCTCTCTGGCTGGCTACCCCCTGAACTACTGGGTCTCTGGACCCATTGTGCCCAGCCACCCC
  CAAAAGCCCTCAGGCGAGAGCTGCCTGAGGAGGCAGCCGCTGAGGAGGAAAGGAGAAAGATTGAAGTTCC
  AAGTGAGATTGAGGTCCCGAGGGAGGCCCTGGAGCCCAGTGTTCCCCTTATGGTGTCTTTAGAGATCTCC
  CTAGAGGCAGCTGAAGAGGAGAAGTCCCGCATCAGCCTCATCCCACCAGAAGAACGGTGGGCCTGGCCTG
  AGGTGGAGGCGCCAGAAGCTCCTGCATTGCCCGTGGTGCCTGAACTCCCTGAGGTGCCCATGGAGATGCC
  TTTGGTGCTGCCCCCAGAGCTCGAGCTGCTCTCACTGGAAGCAGTGCACAGGGCAGTGGCACTGGAGCTG
  CAGGCTAACAGGGAGCCCGACTTCAGCAGCCTGGTGTCACCTCTCAGCCCCCGCAGGATGGCTGCCCGGG
  TCTTCTACCTGCTCCTGGTGCTCTCAGCGCAACAGATTCTTCACGTGAAACAAGAAAAGCCATATGGTCG
  CCTCCTGATCCAGCCGGGGCCCAGATTCCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200132 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MFYYPNVLQRHTGCFATIWLAATRGSRLVKREYLRVNVVKTCEEILNYVLVRVQPPQPGLPRPRFSLYLS
  AQLQIGVIRVYSQQCQYLVEDIQHILERLHRAQLQIRIDMETELPSLLLPNHLAMMETLEDAPDPFFGMM
  SVDPRLPSPFDIPQIRHLLEAAIPERVEEIPPEVPTEPREPERIPVTVLPPEAITILEAEPIRMLEIEGE
  RELPEVSRRELDLLIAEEEEAILLEIPRLPPPAPAEVEGIGEALGPEELRLTGWEPGALLMEVTPPEELR
  LPAPPSPERRPPVPPPPRRRRRRRLLFWDKETQISPEKFQEQLQTRAHCWECPMVQPPERTIRGPAELFR
  TPTLSGWLPPELLGLWTHCAQPPPKALRRELPEEAAAEEERRKIEVPSEIEVPREALEPSVPLMVSLEIS
  LEAAEEEKSRISLIPPEERWAWPEVEAPEAPALPVVPELPEVPMEMPLVLPPELELLSLEAVHRAVALEL
  QANREPDFSSLVSPLSPRRMAARVFYLLLVLSAQQILHVKQEKPYGRLLIQPGPRFH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005132
• ORF Size: 1641 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005132.3
• RefSeq Size: 2253 bp
• RefSeq ORF: 1644 bp
• Locus ID: 9985
• UniProt ID: O95072
• Cytogenetics: 14q12
• Domains: Rad21_Rec8, Rad21_Rec8_N
• Protein Pathways: Oocyte meiosis
• MW: 62.6 kDa
• Gene Summary: This gene encodes a member of the kleisin family of SMC (structural maintenance of chromosome) protein partners. The protein localizes to the axial elements of chromosomes during meiosis in both oocytes and spermatocytes. In the mouse, the homologous protein is a key component of the meiotic cohesion complex, which regulates sister chromatid cohesion and recombination between homologous chromosomes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008]