DNAJC17 (NM_018163) Human Tagged ORF Clone

CAT#: RC200130

DNAJC17 (Myc-DDK-tagged)-Human DnaJ (Hsp40) homolog, subfamily C, member 17 (DNAJC17)

Product Images

Circular map for RC200130

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  Western blot validation of overexpression lysate (Cat# LY413260) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200130 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified DNAJC17 protein (Cat# TP300130). The protein was produced from HEK293T cells transfected with DNAJC17 cDNA clone (Cat# RC200130) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DNAJC17
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200130 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGTGACCAAGGAGCTCTTACAGATGGACCTGTACGCGCTGCTAGGCATTGAGGAGAAGGCAGCGG
  ACAAAGAGGTAAAGAAGGCGTATAGGCAGAAGGCCCTCTCCTGCCACCCAGACAAAAATCCAGATAATCC
  CAGAGCAGCTGAACTCTTCCACCAGCTTTCTCAGGCCTTGGAGGTGCTGACCGATGCTGCAGCCAGGGCT
  GCATATGACAAGGTCAGGAAAGCCAAGAAGCAAGCAGCAGAGAGGACCCAGAAACTTGATGAGAAAAGGA
  AGAAAGTGAAGCTTGACCTGGAGGCCCGGGAGCGGCAGGCCCAGGCCCAGGAGAGTGAGGAGGAAGAGGA
  GAGCCGGAGCACCAGGACACTAGAGCAAGAGATCGAACGCCTGAGAGAAGAGGGTTCCCGGCAGCTGGAG
  GAACAGCAGAGGCTCATCCGGGAGCAGATACGCCAGGAGCGTGACCAGAGGTTGAGAGGAAAGGCAGAAA
  ATACTGAAGGCCAAGGAACCCCCAAACTAAAGCTAAAATGGAAGTGCAAGAAGGAGGATGAGTCAAAAGG
  TGGCTACTCCAAAGACGTCCTCCTACGGCTTTTGCAGAAGTATGGTGAGGTTCTCAACCTGGTGCTTTCC
  AGTAAGAAGCCAGGCACTGCTGTGGTGGAGTTTGCAACCGTCAAGGCAGCGGAGCTGGCTGTCCAGAATG
  AAGTTGGCCTGGTGGATAACCCTCTGAAGATTTCCTGGTTGGAGGGACAGCCCCAGGATGCCGTGGGCCG
  CAGCCACTCAGGACTGTCAAAGGGCTCAGTGCTGTCAGAGAGGGACTACGAGAGCCTCGTCATGATGCGC
  ATGCGCCAGGCGGCCGAGCGGCAACAGCTGATCGCACGGATGCAGCAGGAAGACCAGGAGGGGCCGCCTA
  CG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200130 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAVTKELLQMDLYALLGIEEKAADKEVKKAYRQKALSCHPDKNPDNPRAAELFHQLSQALEVLTDAAARA
  AYDKVRKAKKQAAERTQKLDEKRKKVKLDLEARERQAQAQESEEEEESRSTRTLEQEIERLREEGSRQLE
  EQQRLIREQIRQERDQRLRGKAENTEGQGTPKLKLKWKCKKEDESKGGYSKDVLLRLLQKYGEVLNLVLS
  SKKPGTAVVEFATVKAAELAVQNEVGLVDNPLKISWLEGQPQDAVGRSHSGLSKGSVLSERDYESLVMMR
  MRQAAERQQLIARMQQEDQEGPPT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018163
• ORF Size: 912 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018163.3
• RefSeq Size: 1031 bp
• RefSeq ORF: 915 bp
• Locus ID: 55192
• UniProt ID: Q9NVM6
• Cytogenetics: 15q15.1
• MW: 34.7 kDa
• Gene Summary: May negatively affect PAX8-induced thyroglobulin/TG transcription.[UniProtKB/Swiss-Prot Function]