TRIM62 (NM_018207) Human Tagged ORF Clone

CAT#: RC200126

TRIM62 (Myc-DDK-tagged)-Human tripartite motif containing 62 (TRIM62)

Product Images

Circular map for RC200126

• 

  Western blot validation of overexpression lysate (Cat# LY413216) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200126 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TRIM62
• Synonyms: DEAR1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200126 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTGCAGCCTCAAGGACGAGCTGCTGTGCTCCATCTGCCTGAGCATCTACCAGGACCCGGTGAGCC
  TGGGCTGCGAGCACTACTTCTGCCGCCGCTGCATCACGGAGCACTGGGTGCGGCAGGAGGCGCAGGGCGC
  CCGCGACTGCCCCGAGTGCCGGCGCACGTTCGCCGAGCCCGCGCTGGCGCCCAGCCTCAAGCTGGCCAAC
  ATCGTGGAGCGCTACAGCTCCTTCCCGCTGGACGCCATCCTCAACGCGCGCCGCGCCGCGCGACCCTGCC
  AGGCGCACGACAAGGTCAAGCTCTTCTGCCTCACGGACCGCGCGCTTCTCTGCTTCTTCTGCGACGAGCC
  TGCACTGCACGAGCAGCATCAGGTCACCGGCATCGACGACGCCTTCGACGAGCTGCAGAGGGAGCTGAAG
  GACCAACTTCAGGCCCTTCAAGACAGCGAGCGGGAACACACCGAAGCGCTGCAGCTGCTCAAGCGACAAC
  TGGCGGAGACCAAGTCTTCCACCAAGAGCCTGCGGACCACTATCGGCGAGGCCTTCGAGCGGCTGCACCG
  GCTGCTGCGTGAACGCCAGAAGGCCATGCTAGAGGAGCTGGAGGCGGACACGGCCCGCACGCTGACCGAC
  ATCGAGCAGAAAGTCCAGCGCTACAGCCAGCAGCTGCGCAAGGTCCAGGAGGGAGCCCAGATCCTGCAGG
  AGCGGCTGGCTGAAACCGACCGGCACACCTTCCTGGCTGGGGTGGCCTCACTGTCCGAGCGGCTCAAGGG
  AAAAATCCATGAGACCAACCTCACATATGAAGACTTCCCGACCTCCAAGTACACAGGCCCCCTGCAGTAC
  ACCATCTGGAAGTCCCTGTTCCAGGACATCCACCCAGTGCCAGCCGCCCTAACCCTGGACCCGGGCACAG
  CCCACCAGCGCCTGATCCTGTCGGACGACTGCACCATTGTGGCTTACGGCAACTTGCACCCACAGCCACT
  GCAGGACTCGCCAAAGCGCTTCGATGTGGAGGTGTCGGTGCTGGGTTCTGAAGCCTTCAGTAGTGGCGTC
  CACTACTGGGAGGTGGTGGTGGCGGAGAAGACCCAGTGGGTGATCGGGCTGGCACACGAAGCCGCAAGCC
  GCAAGGGCAGCATCCAGATCCAGCCCAGCCGCGGCTTCTACTGCATCGTGATGCACGATGGCAACCAGTA
  CAGCGCCTGCACGGAGCCCTGGACGCGGCTTAACGTCCGGGACAAGCTTGACAAGGTGGGTGTCTTCCTG
  GACTATGACCAAGGCTTGCTCATCTTCTACAATGCTGATGACATGTCCTGGCTCTACACCTTCCGCGAGA
  AGTTCCCTGGCAAGCTCTGCTCTTACTTCAGCCCTGGCCAGAGCCACGCCAATGGCAAGAACGTTCAGCC
  GCTGCGGATCAACACCGTCCGCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200126 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MACSLKDELLCSICLSIYQDPVSLGCEHYFCRRCITEHWVRQEAQGARDCPECRRTFAEPALAPSLKLAN
  IVERYSSFPLDAILNARRAARPCQAHDKVKLFCLTDRALLCFFCDEPALHEQHQVTGIDDAFDELQRELK
  DQLQALQDSEREHTEALQLLKRQLAETKSSTKSLRTTIGEAFERLHRLLRERQKAMLEELEADTARTLTD
  IEQKVQRYSQQLRKVQEGAQILQERLAETDRHTFLAGVASLSERLKGKIHETNLTYEDFPTSKYTGPLQY
  TIWKSLFQDIHPVPAALTLDPGTAHQRLILSDDCTIVAYGNLHPQPLQDSPKRFDVEVSVLGSEAFSSGV
  HYWEVVVAEKTQWVIGLAHEAASRKGSIQIQPSRGFYCIVMHDGNQYSACTEPWTRLNVRDKLDKVGVFL
  DYDQGLLIFYNADDMSWLYTFREKFPGKLCSYFSPGQSHANGKNVQPLRINTVRI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018207
• ORF Size: 1425 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018207.3
• RefSeq Size: 3865 bp
• RefSeq ORF: 1428 bp
• Locus ID: 55223
• UniProt ID: Q9BVG3
• Cytogenetics: 1p35.1
• Domains: zf-B_box, RING, SPRY, PRY
• Protein Families: Druggable Genome
• MW: 54.3 kDa
• Gene Summary: E3 ubiquitin ligase whose activity is dependent on E2 ubiquitin-conjugating enzyme UBE2D2.[UniProtKB/Swiss-Prot Function]