TRIM62 (NM_018207) Human Tagged ORF Clone

CAT#: RC200126

Reviews ()
Write a review

TRIM62 (Myc-DDK-tagged)-Human tripartite motif containing 62 (TRIM62)



  "NM_018207" in other vectors (5)

Reconstitution Protocol

USD 457.00

USD 686.00


Availability*
In Stock

Size
    • 10 ug


Product images

Other products for "TRIM62"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol TRIM62
Synonyms DEAR1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC200126 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTGCAGCCTCAAGGACGAGCTGCTGTGCTCCATCTGCCTGAGCATCTACCAGGACCCGGTGAGCC
TGGGCTGCGAGCACTACTTCTGCCGCCGCTGCATCACGGAGCACTGGGTGCGGCAGGAGGCGCAGGGCGC
CCGCGACTGCCCCGAGTGCCGGCGCACGTTCGCCGAGCCCGCGCTGGCGCCCAGCCTCAAGCTGGCCAAC
ATCGTGGAGCGCTACAGCTCCTTCCCGCTGGACGCCATCCTCAACGCGCGCCGCGCCGCGCGACCCTGCC
AGGCGCACGACAAGGTCAAGCTCTTCTGCCTCACGGACCGCGCGCTTCTCTGCTTCTTCTGCGACGAGCC
TGCACTGCACGAGCAGCATCAGGTCACCGGCATCGACGACGCCTTCGACGAGCTGCAGAGGGAGCTGAAG
GACCAACTTCAGGCCCTTCAAGACAGCGAGCGGGAACACACCGAAGCGCTGCAGCTGCTCAAGCGACAAC
TGGCGGAGACCAAGTCTTCCACCAAGAGCCTGCGGACCACTATCGGCGAGGCCTTCGAGCGGCTGCACCG
GCTGCTGCGTGAACGCCAGAAGGCCATGCTAGAGGAGCTGGAGGCGGACACGGCCCGCACGCTGACCGAC
ATCGAGCAGAAAGTCCAGCGCTACAGCCAGCAGCTGCGCAAGGTCCAGGAGGGAGCCCAGATCCTGCAGG
AGCGGCTGGCTGAAACCGACCGGCACACCTTCCTGGCTGGGGTGGCCTCACTGTCCGAGCGGCTCAAGGG
AAAAATCCATGAGACCAACCTCACATATGAAGACTTCCCGACCTCCAAGTACACAGGCCCCCTGCAGTAC
ACCATCTGGAAGTCCCTGTTCCAGGACATCCACCCAGTGCCAGCCGCCCTAACCCTGGACCCGGGCACAG
CCCACCAGCGCCTGATCCTGTCGGACGACTGCACCATTGTGGCTTACGGCAACTTGCACCCACAGCCACT
GCAGGACTCGCCAAAGCGCTTCGATGTGGAGGTGTCGGTGCTGGGTTCTGAAGCCTTCAGTAGTGGCGTC
CACTACTGGGAGGTGGTGGTGGCGGAGAAGACCCAGTGGGTGATCGGGCTGGCACACGAAGCCGCAAGCC
GCAAGGGCAGCATCCAGATCCAGCCCAGCCGCGGCTTCTACTGCATCGTGATGCACGATGGCAACCAGTA
CAGCGCCTGCACGGAGCCCTGGACGCGGCTTAACGTCCGGGACAAGCTTGACAAGGTGGGTGTCTTCCTG
GACTATGACCAAGGCTTGCTCATCTTCTACAATGCTGATGACATGTCCTGGCTCTACACCTTCCGCGAGA
AGTTCCCTGGCAAGCTCTGCTCTTACTTCAGCCCTGGCCAGAGCCACGCCAATGGCAAGAACGTTCAGCC
GCTGCGGATCAACACCGTCCGCATC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC200126 protein sequence
Red=Cloning site Green=Tags(s)

MACSLKDELLCSICLSIYQDPVSLGCEHYFCRRCITEHWVRQEAQGARDCPECRRTFAEPALAPSLKLAN
IVERYSSFPLDAILNARRAARPCQAHDKVKLFCLTDRALLCFFCDEPALHEQHQVTGIDDAFDELQRELK
DQLQALQDSEREHTEALQLLKRQLAETKSSTKSLRTTIGEAFERLHRLLRERQKAMLEELEADTARTLTD
IEQKVQRYSQQLRKVQEGAQILQERLAETDRHTFLAGVASLSERLKGKIHETNLTYEDFPTSKYTGPLQY
TIWKSLFQDIHPVPAALTLDPGTAHQRLILSDDCTIVAYGNLHPQPLQDSPKRFDVEVSVLGSEAFSSGV
HYWEVVVAEKTQWVIGLAHEAASRKGSIQIQPSRGFYCIVMHDGNQYSACTEPWTRLNVRDKLDKVGVFL
DYDQGLLIFYNADDMSWLYTFREKFPGKLCSYFSPGQSHANGKNVQPLRINTVRI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_018207
ORF Size 1425 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_018207.3
RefSeq Size 3865 bp
RefSeq ORF 1428 bp
Locus ID 55223
UniProt ID Q9BVG3
Cytogenetics 1p35.1
Domains zf-B_box, RING, SPRY, PRY
Protein Families Druggable Genome
MW 54.3 kDa
Gene Summary E3 ubiquitin ligase whose activity is dependent on E2 ubiquitin-conjugating enzyme UBE2D2.[UniProtKB/Swiss-Prot Function]

Other Versions

Frequently bought together (4)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 30 ul

USD 177.00


pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

USD 650.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

USD 160.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 59.00

Customer Reviews 
Loading...
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.