C20orf31(EDEM2) (NM_018217) Human Tagged ORF Clone

CAT#: RC200124

EDEM2 (Myc-DDK-tagged)-Human ER degradation enhancer, mannosidase alpha-like 2 (EDEM2), transcript variant 1

Clone Modification / Custom Cloning Service

USD 98.00

USD 560.00


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Size
    • 10 ug

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Specifications

Product Data
Product Name C20orf31(EDEM2) (NM_018217) Human Tagged ORF Clone
Symbol EDEM2
Synonyms bA4204.1; C20orf31; C20orf49
Vector pCMV6-Entry
Sequence Data
ORF Nucleotide Sequence
>RC200124 representing NM_018217
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCTTTCCGGCTGCTCATCCCGCTCGGCCTCCTGTGCGCGCTGCTGCCTCAGCACCATGGTGCGCCAG
GTCCCGACGGCTCCGCGCCAGATCCCGCCCACTACAGGGAGCGAGTCAAGGCCATGTTCTACCACGCCTA
CGACAGCTACCTGGAGAATGCCTTTCCCTTCGATGAGCTGCGACCTCTCACCTGTGACGGGCACGACACC
TGGGGCAGTTTTTCTCTGACTCTAATTGATGCACTGGACACCTTGCTGATTTTGGGGAATGTCTCAGAAT
TCCAAAGAGTGGTTGAAGTGCTCCAGGACAGCGTGGACTTTGATATTGATGTGAACGCCTCTGTGTTTGA
AACAAACATTCGAGTGGTAGGAGGACTCCTGTCTGCTCATCTGCTCTCCAAGAAGGCTGGGGTGGAAGTA
GAGGCTGGATGGCCCTGTTCCGGGCCTCTCCTGAGAATGGCTGAGGAGGCGGCCCGAAAACTCCTCCCAG
CCTTTCAGACCCCCACTGGCATGCCATATGGAACAGTGAACTTACTTCATGGCGTGAACCCAGGAGAGAC
CCCTGTCACCTGTACGGCAGGGATTGGGACCTTCATTGTTGAATTTGCCACCCTGAGCAGCCTCACTGGT
GACCCGGTGTTCGAAGATGTGGCCAGAGTGGCTTTGATGCGCCTCTGGGAGAGCCGGTCAGATATCGGGC
TGGTCGGCAACCACATTGATGTGCTCACTGGCAAGTGGGTGGCCCAGGACGCAGGCATCGGGGCTGGCGT
GGACTCCTACTTTGAGTACTTGGTGAAAGGAGCCATCCTGCTTCAGGATAAGAAGCTCATGGCCATGTTC
CTAGAGTATAACAAAGCCATCCGGAACTACACCCGCTTCGATGACTGGTACCTGTGGGTTCAGATGTACA
AGGGGACTGTGTCCATGCCAGTCTTCCAGTCCTTGGAGGCCTACTGGCCTGGTCTTCAGAGCCTCATTGG
AGACATTGACAATGCCATGAGGACCTTCCTCAACTACTACACTGTATGGAAGCAGTTTGGGGGGCTCCCG
GAATTCTACAACATTCCTCAGGGATACACAGTGGAGAAGCGAGAGGGCTACCCACTTCGGCCAGAACTTA
TTGAAAGCGCAATGTACCTCTACCGTGCCACGGGGGATCCCACCCTCCTAGAACTCGGAAGAGATGCTGT
GGAATCCATTGAAAAAATCAGCAAGGTGGAGTGCGGATTTGCAACAATCAAAGATCTGCGAGACCACAAG
CTGGACAACCGCATGGAGTCGTTCTTCCTGGCCGAGACTGTGAAATACCTCTACCTCCTGTTTGACCCAA
CCAACTTCATCCACAACAATGGGTCCACCTTCGACACGGTGATCACCCCCTATGGGGAGTGCATCCTGGG
GGCTGGGGGGTACATCTTCAACACAGAAGCTCACCCCATCGACCCTGCCGCCCTGCACTGCTGCCAGAGG
CTGAAGGAAGAGCAGTGGGAGGTGGAGGACTTGATGAGGGAATTCTACTCTCTCAAACGGAGCAGGTCGA
AATTTCAGAAAAACACTGTTAGTTCGGGGCCATGGGAACCTCCAGCAAGGCCAGGAACACTCTTCTCACC
AGAAAACCATGACCAGGCAAGGGAGAGGAAGCCTGCCAAACAGAAGGTCCCACTTCTCAGCTGCCCCAGT
CAGCCCTTCACCTCCAAGTTGGCATTACTGGGACAGGTTTTCCTAGACTCCTCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC200124 representing NM_018217
Red=Cloning site Green=Tags(s)

MPFRLLIPLGLLCALLPQHHGAPGPDGSAPDPAHYRERVKAMFYHAYDSYLENAFPFDELRPLTCDGHDT
WGSFSLTLIDALDTLLILGNVSEFQRVVEVLQDSVDFDIDVNASVFETNIRVVGGLLSAHLLSKKAGVEV
EAGWPCSGPLLRMAEEAARKLLPAFQTPTGMPYGTVNLLHGVNPGETPVTCTAGIGTFIVEFATLSSLTG
DPVFEDVARVALMRLWESRSDIGLVGNHIDVLTGKWVAQDAGIGAGVDSYFEYLVKGAILLQDKKLMAMF
LEYNKAIRNYTRFDDWYLWVQMYKGTVSMPVFQSLEAYWPGLQSLIGDIDNAMRTFLNYYTVWKQFGGLP
EFYNIPQGYTVEKREGYPLRPELIESAMYLYRATGDPTLLELGRDAVESIEKISKVECGFATIKDLRDHK
LDNRMESFFLAETVKYLYLLFDPTNFIHNNGSTFDTVITPYGECILGAGGYIFNTEAHPIDPAALHCCQR
LKEEQWEVEDLMREFYSLKRSRSKFQKNTVSSGPWEPPARPGTLFSPENHDQARERKPAKQKVPLLSCPS
QPFTSKLALLGQVFLDSS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_018217
ORF Size 1734 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reference Data
RefSeq NM_018217.1, NM_018217.2, NP_060687
RefSeq Size 1885
RefSeq ORF 1737
Locus ID 55741
Cytogenetics 20q11.22
Domains Glyco_hydro_47
Protein Families Secreted Protein
MW 64.6 kDa
Gene Summary In the endoplasmic reticulum (ER), misfolded proteins are retrotranslocated to the cytosol and degraded by the proteasome in a process known as ER-associated degradation (ERAD). EDEM2 belongs to a family of proteins involved in ERAD of glycoproteins (Mast et al., 2005 [PubMed 15537790]).[supplied by OMIM, Mar 2008].
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Citations (1)