KLHDC2 (NM_014315) Human Tagged ORF Clone

CAT#: RC200116

KLHDC2 (Myc-DDK-tagged)-Human kelch domain containing 2 (KLHDC2)

Product Images

Western blot validation of overexpression lysate (Cat# LY402313) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200116 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified KLHDC2 protein (Cat# TP300116). The protein was produced from HEK293T cells transfected with KLHDC2 cDNA clone (Cat# RC200116) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KLHDC2
• Synonyms: HCLP-1; HCLP1; LCP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200116 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGATGGCAACGAGGATCTGCGGGCTGACGACTTGCCTGGGCCAGCCTTCGAGAGCTATGAGTCCA
  TGGAGCTTGCCTGCCCCGCTGAGCGCAGCGGCCACGTAGCCGTCAGCGACGGGCGCCACATGTTCGTCTG
  GGGCGGCTACAAGAGTAATCAAGTCAGAGGATTATATGACTTTTATCTGCCTAGAGAAGAACTATGGATC
  TACAACATGGAGACTGGAAGATGGAAAAAAATCAACACTGAAGGTGATGTTCCTCCTTCTATGTCAGGAA
  GCTGTGCTGTGTGTGTAGACAGGGTGCTGTACTTGTTTGGAGGACACCATTCAAGAGGCAATACCAATAA
  GTTCTACATGCTGGATTCAAGGTCTACAGACAGAGTGTTACAGTGGGAAAGAATTGATTGCCAAGGAATT
  CCTCCATCATCAAAGGACAAACTTGGTGTCTGGGTATATAAAAACAAGTTAATATTTTTTGGAGGGTATG
  GATATTTGCCTGAAGATAAAGTATTGGGAACTTTTGAATTCGATGAAACATCTTTTTGGAATTCAAGTCA
  TCCAAGAGGATGGAATGATCATGTACATATTTTAGATACTGAAACATTTACCTGGAGCCAGCCTATAACT
  ACTGGTAAAGCACCTTCACCTCGTGCTGCCCATGCCTGTGCAACTGTCGGAAATAGAGGCTTCGTGTTTG
  GAGGCAGATATCGAGATGCTAGAATGAATGATCTTCACTATCTTAATCTGGATACATGGGAGTGGAATGA
  ATTAATTCCACAAGGCATATGCCCAGTTGGTCGATCTTGGCACTCACTAACACCAGTTTCTTCAGATCAT
  CTTTTTCTCTTTGGAGGATTTACCACTGATAAACAGCCACTAAGTGATGCCTGGACTTACTGCATCAGTA
  AAAATGAATGGATACAATTTAATCATCCATATACCGAAAAACCAAGGTTATGGCACACAGCTTGTGCCAG
  CGATGAAGGAGAAGTAATTGTTTTTGGTGGATGTGCCAACAACTTGCTTGTCCATCACAGAGCTGCACAC
  AGTAATGAAATACTAATATTTTCAGTTCAACCAAAATCTCTTGTACGGCTAAGCTTAGAAGCAGTCATTT
  GCTTTAAAGAAATGTTAGCCAACTCATGGAACTGCCTTCCAAAACACTTACTTCACAGTGTTAATCAGAG
  GTTTGGTAGTAACAACACTTCTGGATCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200116 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MADGNEDLRADDLPGPAFESYESMELACPAERSGHVAVSDGRHMFVWGGYKSNQVRGLYDFYLPREELWI
  YNMETGRWKKINTEGDVPPSMSGSCAVCVDRVLYLFGGHHSRGNTNKFYMLDSRSTDRVLQWERIDCQGI
  PPSSKDKLGVWVYKNKLIFFGGYGYLPEDKVLGTFEFDETSFWNSSHPRGWNDHVHILDTETFTWSQPIT
  TGKAPSPRAAHACATVGNRGFVFGGRYRDARMNDLHYLNLDTWEWNELIPQGICPVGRSWHSLTPVSSDH
  LFLFGGFTTDKQPLSDAWTYCISKNEWIQFNHPYTEKPRLWHTACASDEGEVIVFGGCANNLLVHHRAAH
  SNEILIFSVQPKSLVRLSLEAVICFKEMLANSWNCLPKHLLHSVNQRFGSNNTSGS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014315
• ORF Size: 1218 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014315.3
• RefSeq Size: 1808 bp
• RefSeq ORF: 1221 bp
• Locus ID: 23588
• UniProt ID: Q9Y2U9
• Cytogenetics: 14q21.3
• Domains: Kelch
• MW: 46.1 kDa
• Gene Summary: Represses CREB3-mediated transcription by interfering with CREB3-DNA binding.[UniProtKB/Swiss-Prot Function]