SWAP70 (NM_015055) Human Tagged ORF Clone

CAT#: RC200074

SWAP70 (Myc-DDK-tagged)-Human SWAP switching B-cell complex 70kDa subunit (SWAP70)

Product Images

Circular map for RC200074

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  Western blot validation of overexpression lysate (Cat# LY414823) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200074 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SWAP70 protein (Cat# TP300074). The protein was produced from HEK293T cells transfected with SWAP70 cDNA clone (Cat# RC200074) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SWAP70
• Synonyms: HSPC321; SWAP-70
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200074 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAGCTTGAAGGAGGAGCTGCTCAAAGCCATCTGGCACGCCTTCACCGCACTCGACCAGGACCACA
  GCGGCAAGGTCTCCAAGTCCCAGCTCAAGGTCCTTTCCCATAACCTGTGCACGGTGCTGAAGGTTCCTCA
  TGACCCAGTTGCCCTTGAAGAGCACTTCAGGGATGATGATGAGGGTCCAGTGTCCAACCAGGGCTACATG
  CCTTATTTAAACAGGTTCATTTTGGAAAAGGTCCAAGACAACTTTGACAAGATTGAATTCAATAGGATGT
  GTTGGACCCTCTGTGTCAAAAAAAACCTCACAAAGAATCCCCTGCTCATTACAGAAGAAGATGCATTTAA
  AATATGGGTTATTTTCAACTTTTTATCTGAGGACAAGTATCCATTAATTATTGTGTCAGAAGAGATTGAA
  TACCTGCTTAAGAAGCTTACAGAAGCTATGGGAGGAGGTTGGCAGCAAGAACAATTTGAACATTATAAAA
  TCAACTTTGATGACAGTAAAAATGGCCTTTCTGCATGGGAACTTATTGAGCTTATTGGAAATGGACAGTT
  TAGCAAAGGCATGGACCGGCAGACTGTGTCTATGGCAATTAATGAAGTCTTTAATGAACTTATATTAGAT
  GTGTTAAAGCAGGGTTACATGATGAAAAAGGGCCACAGACGGAAAAACTGGACTGAAAGATGGTTTGTAC
  TAAAACCCAACATAATTTCTTACTATGTGAGTGAGGATCTGAAGGATAAGAAAGGAGACATTCTCTTGGA
  TGAAAATTGCTGTGTAGAGTCCTTGCCTGACAAAGATGGAAAGAAATGCCTTTTTCTCGTAAAATGTTTT
  GATAAGACTTTTGAAATCAGTGCTTCAGATAAGAAGAAGAAACAGGAGTGGATTCAAGCCATTCATTCTA
  CTATTCATCTGTTGAAGCTGGGCAGCCCTCCACCACACAAAGAAGCCCGCCAGCGTCGGAAAGAACTCCG
  GAAGAAGCAGCTGGCTGAACAAGAGGAACTGGAGCGACAAATGAAGGAACTCCAGGCCGCCAACGAAAGC
  AAGCAGCAGGAGCTGGAGGCCGTGCGGAAGAAACTGGAGGAAGCAGCATCTCGTGCAGCAGAAGAGGAAA
  AGAAACGCCTTCAGACTCAAGTGGAACTTCAGGCCAGGTTCAGCACAGAGCTGGAAAGAGAGAAGCTTAT
  CAGACAGCAGATGGAAGAACAGGTTGCTCAAAAGTCCTCTGAACTGGAACAGTATTTACAGCGAGTACGG
  GAGCTGGAAGACATGTACCTAAAGCTGCAGGAGGCTCTTGAAGATGAGAGACAGGCCCGGCAAGATGAAG
  AGACAGTGCGGAAGCTTCAGGCCAGGTTGTTGGAGGAAGAGTCTTCCAAGAGGGCTGAACTAGAAAAGTG
  GCACTTGGAGCAGCAGCAGGCCATTCAGACAACCGAGGCGGAGAAGCAGGAGTTGGAGAATCAGCGTGTC
  CTGAAGGAACAGGCCCTGCAGGAGGCCATGGAGCAGCTGGAGCAGCTTGAGTTAGAACGGAAGCAAGCAC
  TTGAGCAGTACGAGGAAGTTAAAAAGAAGCTGGAGATGGCAACTAATAAGACCAAGAGCTGGAAGGACAA
  AGTGGCCCATCATGAAGGATTAATTCGACTGATAGAACCAGGTTCAAAGAACCCTCACCTGATCACTAAC
  TGGGGACCTGCAGCTTTCACTGAGGCAGAACTTGAAGAGAGAGAGAAGAACTGGAAAGAGAAAAAGACCA
  CGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200074 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGSLKEELLKAIWHAFTALDQDHSGKVSKSQLKVLSHNLCTVLKVPHDPVALEEHFRDDDEGPVSNQGYM
  PYLNRFILEKVQDNFDKIEFNRMCWTLCVKKNLTKNPLLITEEDAFKIWVIFNFLSEDKYPLIIVSEEIE
  YLLKKLTEAMGGGWQQEQFEHYKINFDDSKNGLSAWELIELIGNGQFSKGMDRQTVSMAINEVFNELILD
  VLKQGYMMKKGHRRKNWTERWFVLKPNIISYYVSEDLKDKKGDILLDENCCVESLPDKDGKKCLFLVKCF
  DKTFEISASDKKKKQEWIQAIHSTIHLLKLGSPPPHKEARQRRKELRKKQLAEQEELERQMKELQAANES
  KQQELEAVRKKLEEAASRAAEEEKKRLQTQVELQARFSTELEREKLIRQQMEEQVAQKSSELEQYLQRVR
  ELEDMYLKLQEALEDERQARQDEETVRKLQARLLEEESSKRAELEKWHLEQQQAIQTTEAEKQELENQRV
  LKEQALQEAMEQLEQLELERKQALEQYEEVKKKLEMATNKTKSWKDKVAHHEGLIRLIEPGSKNPHLITN
  WGPAAFTEAELEEREKNWKEKKTTE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015055
• ORF Size: 1755 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015055.4
• RefSeq Size: 4882 bp
• RefSeq ORF: 1758 bp
• Locus ID: 23075
• UniProt ID: Q9UH65
• Cytogenetics: 11p15.4
• MW: 69 kDa
• Gene Summary: Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion (By similarity).[UniProtKB/Swiss-Prot Function]