TMEM50B (NM_006134) Human Tagged ORF Clone

CAT#: RC200002

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TMEM50B (Myc-DDK-tagged)-Human transmembrane protein 50B (TMEM50B)



Reconstitution Protocol

USD 150.00

USD 225.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol TMEM50B
Synonyms C21orf4; HCVP7TP3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC200002 representing NM_006134.
Blue=ORF Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGCAGGCTTCCTAGATAATTTTCGTTGGCCAGAATGTGAATGTATTGACTGGAGTGAGAGAAGAAAT
GCTGTGGCATCTGTTGTCGCAGGTATATTGTTTTTTACAGGCTGGTGGATAATGATTGATGCAGCTGTG
GTGTATCCTAAGCCAGAACAGTTGAACCATGCCTTTCACACATGTGGTGTATTTTCCACATTGGCTTTC
TTCATGATAAATGCTGTATCCAATGCTCAGGTGAGAGGTGATAGCTATGAAAGCGGCTGTTTAGGAAGA
ACAGGTGCTCGAGTTTGGCTTTTCATTGGTTTCATGTTGATGTTTGGGTCACTTATTGCTTCCATGTGG
ATTCTTTTTGGTGCATATGTTACCCAAAATACTGATGTTTATCCGGGACTAGCTGTGTTTTTTCAAAAT
GCACTTATATTTTTTAGCACTCTGATCTACAAATTTGGAAGAACCGAAGAGCTATGGACC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
>Peptide sequence encoded by RC200002
Blue=ORF Red=Cloning site Green=Tag(s)

MAGFLDNFRWPECECIDWSERRNAVASVVAGILFFTGWWIMIDAAVVYPKPEQLNHAFHTCGVFSTLAF
FMINAVSNAQVRGDSYESGCLGRTGARVWLFIGFMLMFGSLIASMWILFGAYVTQNTDVYPGLAVFFQN
ALIFFSTLIYKFGRTEELWT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_006134
ORF Size 474 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_006134.7
RefSeq Size 2385 bp
RefSeq ORF 477 bp
Locus ID 757
UniProt ID P56557
Cytogenetics 21q22.11
Protein Families Druggable Genome, Transmembrane
MW 17.9 kDa
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