Polr2a (NM_001291068) Mouse Tagged ORF Clone
CAT#: MR231897
- TrueORF®
Polr2a (myc-DDK-tagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide A (Polr2a)
"NM_001291068" in other vectors (1)
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USD 198.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Polr2a |
Synonyms | 220kDa; Rpb1; Rpo2-1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR231897 representing NM_001291068
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACGGGGGTGGCCCCCCCTCCGGGGACAGCGCATGCCCGCTGCGCACCATCAAGAGAGTGCAGTTCG GAGTCCTGAGTCCGGATGAATTGAAGCGGATGTCTGTGACAGAGGGTGGTATCAAATACCCAGAAACAAC GGAGGGAGGTCGCCCCAAACTTGGGGGACTAATGGATCCACGGCAGGGGGTGATTGAGCGGACTGGCCGC TGCCAAACATGTGCAGGAAACATGACCGAGTGTCCTGGCCACTTTGGCCACATCGAATTGGCCAAACCTG TGTTTCATGTGGGCTTCCTGGTGAAGACAATGAAGGTTTTGCGCTGTGTCTGCTTCTTCTGCTCCAAACT GCTTGTAGATTCTAATAACCCGAAGATTAAGGACATCCTGGCCAAATCTAAGGGGCAGCCAAAGAAACGG CTTACGCATGTCTATGACCTGTGCAAGGGCAAAAACATCTGTGAAGGTGGAGAGGAGATGGACAACAAGT TTGGTGTGGAGCAGCCGGAGGGTGATGAGGATCTGACCAAAGAGAAGGGCCATGGCGGCTGTGGGCGGTA CCAACCCCGAATCCGGCGCTCTGGCCTAGAGTTGTATGCTGAATGGAAGCATGTTAATGAGGACTCTCAG GAGAAAAAGATCTTGCTGAGTCCAGAACGAGTGCATGAAATCTTCAAACGCATCTCAGATGAGGAGTGTT TTGTGTTGGGCATGGAGCCTCGCTATGCCCGGCCTGAGTGGATGATTGTCACAGTGTTGCCTGTGCCTCC TCTCTCAGTGAGACCTGCTGTGGTGATGCAGGGTTCTGCTCGAAACCAGGATGATCTGACTCACAAACTG GCTGACATTGTGAAGATCAACAATCAGCTGCGGCGGAACGAGCAGAATGGTGCAGCTGCCCATGTCATAG CAGAAGATGTAAAACTTCTCCAGTTCCATGTGGCCACCATGGTGGACAATGAACTGCCCGGCTTACCCCG TGCCATGCAGAAGTCTGGCCGTCCCCTCAAGTCCCTGAAGCAACGGTTGAAGGGCAAAGAAGGACGGGTT CGAGGGAATCTAATGGGCAAGCGAGTGGACTTCTCAGCCCGCACTGTCATCACCCCTGACCCTAACCTAT CCATTGACCAAGTGGGTGTGCCCCGCTCCATTGCTGCCAACATGACCTTTGCAGAGATTGTCACACCCTT CAATATTGACAGACTTCAGGAATTAGTACGCAGGGGAAACAGCCAGTATCCAGGAGCCAAGTACATCATC CGCGATAATGGCGATCGAATTGACTTGCGTTTCCATCCTAAGCCCAGTGACCTTCATCTGCAGACTGGCT ATAAGGTGGAACGGCACATGTGTGATGGGGACATTGTTATCTTCAATCGTCAGCCAACTTTGCACAAAAT GTCCATGATGGGGCATCGGGTCCGAATCCTCCCCTGGTCTACTTTTCGGTTGAATCTTAGTGTGACAACT CCATACAATGCAGACTTTGATGGAGATGAGATGAATTTGCATCTGCCACAGTCTCTGGAAACACGGGCAG AGATCCAAGAGTTGGCTATGGTGCCTCGAATGATTGTCACCCCCCAGAGCAATCGACCTGTCATGGGTAT TGTGCAAGACACACTCACGGCAGTACGCAAATTCACTAAGAGAGATGTCTTCCTGGAGCGGGGTGAAGTC ATGAACCTCTTAATGTTTCTGTCCACGTGGGATGGCAAGGTCCCACAACCAGCTATCCTCAAGCCCCGGC CCCTGTGGACAGGCAAGCAGATCTTCTCCCTCATCATACCTGGACACATCAATTGTATCCGTACCCACAG CACCCATCCTGATGATGAAGATAGTGGCCCTTACAAGCACATCTCTCCTGGGGACACCAAAGTTGTAGTG GAGAATGGGGAGCTTATCATGGGCATCCTTTGTAAGAAGTCCCTGGGCACCTCAGCAGGCTCGCTGGTCC ATATCTCCTACCTGGAGATGGGGCATGACATCACCCGCCTCTTCTACTCCAACATTCAGACTGTCATTAA CAACTGGCTTCTCATCGAGGGTCATACCATTGGCATTGGGGACTCCATTGCAGATTCAAAGACTTACCAG GACATTCAGAACACTATTAAGAAAGCTAAACAAGATGTAATAGAGGTCATTGAGAAGGCTCATAACAATG AGCTAGAACCCACTCCAGGAAACACATTGCGTCAGACATTTGAGAATCAAGTGAATCGTATTCTCAATGA TGCTCGAGACAAAACTGGCTCCTCTGCACAGAAATCCCTCTCTGAATATAACAACTTCAAGTCTATGGTG GTGTCTGGAGCCAAGGGTTCCAAGATCAACATCTCCCAGGTCATTGCTGTTGTCGGGCAGCAGAATGTAG AGGGCAAGCGGATCCCATTTGGATTCAAGCATCGGACTCTTCCTCACTTTATCAAGGATGATTATGGTCC TGAGAGCCGAGGCTTTGTAGAAAATTCATACTTGGCTGGTCTCACACCCACTGAGTTCTTCTTCCATGCC ATGGGAGGACGAGAAGGTCTCATTGACACGGCTGTTAAGACTGCTGAGACTGGGTATATTCAGCGAAGGC TGATAAAGTCCATGGAGTCGGTGATGGTGAAATATGATGCAACTGTGAGGAACTCCATCAACCAGGTGGT ACAGCTGCGCTATGGAGAGGACGGCCTGGCAGGGGAGAGTGTTGAGTTCCAGAATCTGGCTACACTTAAA CCTTCTAATAAAGCTTTTGAGAAGAAGTTCCGTTTTGATTATACCAATGAGAGGGCCCTGCGGCGCACCC TTCAGGAGGACCTGGTAAAGGATGTGCTGAGCAATGCCCATATACAGAATGAGCTGGAACGGGAATTTGA ACGGATGCGTGAAGATCGGGAAGTGCTCAGGGTCATCTTCCCAACTGGTGACAGCAAGGTGGTCCTCCCT TGTAACCTTCTGCGGATGATCTGGAATGCTCAGAAAATCTTCCACATCAACCCTCGCCTTCCCTCTGACC TGCATCCCATCAAAGTGGTAGAGGGAGTCAAAGAATTGAGCAAGAAGTTGGTAATTGTGAATGGAGATGA CCCACTAAGTCGGCAGGCACAGGAGAATGCCACATTGCTCTTCAACATCCACCTAAGATCCACACTCTGC TCTCGCCGCATGGCTGAGGAGTTTCGGCTGAGTGGAGAGGCCTTTGACTGGCTGCTTGGGGAGATAGAGT CCAAGTTCAACCAAGCCATTGCCCATCCTGGAGAAATGGTGGGAGCTCTGGCTGCTCAGTCTCTTGGAGA ACCTGCCACTCAGATGACCCTGAACACTTTCCACTATGCTGGTGTATCTGCCAAGAATGTGACGCTGGGT GTGCCTCGACTTAAGGAGCTTATCAACATTTCCAAGAAGCCAAAGACTCCTTCACTCACTGTCTTCCTGT TAGGTCAATCTGCACGAGATGCTGAAAGAGCCAAGGACATTCTTTGCCGCTTGGAACATACAACATTGAG GAAGGTGACTGCCAATACAGCCATCTACTATGACCCTAACCCCCAGAGCACAGTGGTGGCAGAAGATCAG GAGTGGGTGAATGTGTACTATGAGATGCCCGACTTTGACGTGGCCCGGATATCTCCCTGGCTGCTGCGTG TGGAGCTCGACCGAAAGCACATGACTGACCGGAAGCTTACCATGGAACAGATTGCTGAGAAAATCAATGC AGGTTTTGGCGATGATTTGAATTGTATCTTCAACGATGATAATGCAGAGAAGCTGGTCCTTCGAATCCGC ATCATGAACAGTGATGAAAACAAGATGCAAGAGGAGGAAGAGGTGGTGGATAAAATGGATGATGATGTCT TCCTGCGATGCATTGAGTCCAACATGCTGACAGATATGACCCTGCAGGGTATCGAGCAGATCAGCAAGGT GTACATGCACTTACCTCAGACAGACAACAAGAAGAAGATCATCATCACAGAGGATGGGGAGTTCAAGGCC CTGCAGGAATGGATCTTAGAGACGGATGGTGTGAGCCTGATGCGGGTGCTGAGTGAGAAGGATGTAGACC CTGTGCGCACCACGTCCAATGATATTGTGGAGATCTTCACGGTACTGGGCATTGAGGCTGTGCGGAAGGC TCTGGAGCGAGAACTGTACCATGTCATCTCCTTTGATGGTTCTTATGTCAATTATCGGCATTTGGCGCTC CTGTGTGACACTATGACATGCCGTGGCCACTTGATGGCCATCACTCGTCATGGGGTCAACCGCCAGGACA CTGGACCGCTCATGAAATGCTCCTTTGAGGAAACGGTGGATGTCCTTATGGAAGCAGCAGCACATGGGGA GAGTGACCCGATGAAGGGAGTCTCTGAGAATATTATGCTGGGCCAGCTCGCTCCAGCTGGTACTGGCTGT TTTGACCTCCTGCTTGATGCTGAGAAGTGCAAATACGGCATGGAAATCCCCACCAATATCCCTGGCCTGG GGGCTGCTGGACCTACCGGCATGTTCTTTGGCTCTGCACCCAGTCCGATGGGAGGAATATCTCCTGCAAT GACACCCTGGAACCAGGGTGCAACTCCCGCCTATGGTGCCTGGTCCCCGAGTGTTGGGAGCGGGATGACC CCAGGAGCGGCCGGCTTCTCTCCCAGTGCTGCATCTGATGCCAGTGGCTTCAGCCCAGGTTACTCCCCTG CATGGTCTCCCACACCAGGCTCTCCGGGCTCCCCTGGACCCTCAAGCCCATACATCCCCTCACCAGGTGG TGCTATGTCTCCCAGCTACTCACCGACATCACCAGCCTATGAGCCACGCTCCCCTGGGGGCTATACACCC CAGAGCCCCTCCTACTCCCCTACTTCACCTTCCTACTCCCCAACCTCTCCATCTTACTCTCCAACCAGTC CCAACTACAGCCCTACCTCTCCTAGCTACTCGCCCACCTCCCCTAGCTACTCGCCAACCTCTCCTTCCTA CTCCCCCACCTCTCCAAGCTATTCCCCAACCTCTCCTAGCTACTCCCCAACCTCTCCAAGCTATTCTCCA ACATCACCTAGCTATTCTCCAACTTCTCCCAGCTACTCACCGACATCTCCTAGCTACTCCCCAACTTCTC CCAGCTACTCACCAACTTCACCAAGCTATTCTCCCACCTCCCCCAGCTACTCACCGACATCTCCCAGCTA CTCACCAACTTCTCCAAGCTACTCACCAACTTCTCCAAGCTACTCACCCACCAGCCCTAACTATTCTCCA ACTAGTCCCAACTATACCCCGACATCACCCAGCTACAGCCCAACCTCACCCAGTTACTCACCTACAAGTC CCAACTATACACCCACCAGCCCTAATTACAGCCCAACCTCTCCAAGCTATTCCCCAACCTCACCCAGTTA TTCCCCCACCTCACCAAGCTACTCCCCCTCCAGCCCACGATATACACCACAGTCTCCAACCTACACACCA AGCTCACCAAGCTACAGTCCCAGCTCACCAAGCTACAGCCCCACTTCACCCAAGTACACCCCAACTAGTC CTTCCTACAGTCCCAGCTCACCAGAGTACACCCCAGCTTCTCCCAAATACTCACCTACAAGCCCTAAATA TTCACCCACTTCTCCCAAGTATTCTCCTACCAGCCCCACTTACTCACCTACCACCCCAAAATATTCTCCA ACCTCCCCGACATACTCACCAACCTCTCCAGTCTATACCCCGACCTCTCCCAAGTACTCCCCAACCAGCC CTACCTACTCGCCCACTTCTCCCAAGTACTCGCCCACCAGTCCCACCTACTCACCCACCTCTCCCAAGGG CTCCACCTACTCTCCCACTTCTCCTGGCTACTCACCCACTAGCCCCACCTACAGCCTCACCAGCCCAGCC ATCAGCCCAGATGACAGCGATGAGGAGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR231897 representing NM_001291068
Red=Cloning site Green=Tags(s) MHGGGPPSGDSACPLRTIKRVQFGVLSPDELKRMSVTEGGIKYPETTEGGRPKLGGLMDPRQGVIERTGR CQTCAGNMTECPGHFGHIELAKPVFHVGFLVKTMKVLRCVCFFCSKLLVDSNNPKIKDILAKSKGQPKKR LTHVYDLCKGKNICEGGEEMDNKFGVEQPEGDEDLTKEKGHGGCGRYQPRIRRSGLELYAEWKHVNEDSQ EKKILLSPERVHEIFKRISDEECFVLGMEPRYARPEWMIVTVLPVPPLSVRPAVVMQGSARNQDDLTHKL ADIVKINNQLRRNEQNGAAAHVIAEDVKLLQFHVATMVDNELPGLPRAMQKSGRPLKSLKQRLKGKEGRV RGNLMGKRVDFSARTVITPDPNLSIDQVGVPRSIAANMTFAEIVTPFNIDRLQELVRRGNSQYPGAKYII RDNGDRIDLRFHPKPSDLHLQTGYKVERHMCDGDIVIFNRQPTLHKMSMMGHRVRILPWSTFRLNLSVTT PYNADFDGDEMNLHLPQSLETRAEIQELAMVPRMIVTPQSNRPVMGIVQDTLTAVRKFTKRDVFLERGEV MNLLMFLSTWDGKVPQPAILKPRPLWTGKQIFSLIIPGHINCIRTHSTHPDDEDSGPYKHISPGDTKVVV ENGELIMGILCKKSLGTSAGSLVHISYLEMGHDITRLFYSNIQTVINNWLLIEGHTIGIGDSIADSKTYQ DIQNTIKKAKQDVIEVIEKAHNNELEPTPGNTLRQTFENQVNRILNDARDKTGSSAQKSLSEYNNFKSMV VSGAKGSKINISQVIAVVGQQNVEGKRIPFGFKHRTLPHFIKDDYGPESRGFVENSYLAGLTPTEFFFHA MGGREGLIDTAVKTAETGYIQRRLIKSMESVMVKYDATVRNSINQVVQLRYGEDGLAGESVEFQNLATLK PSNKAFEKKFRFDYTNERALRRTLQEDLVKDVLSNAHIQNELEREFERMREDREVLRVIFPTGDSKVVLP CNLLRMIWNAQKIFHINPRLPSDLHPIKVVEGVKELSKKLVIVNGDDPLSRQAQENATLLFNIHLRSTLC SRRMAEEFRLSGEAFDWLLGEIESKFNQAIAHPGEMVGALAAQSLGEPATQMTLNTFHYAGVSAKNVTLG VPRLKELINISKKPKTPSLTVFLLGQSARDAERAKDILCRLEHTTLRKVTANTAIYYDPNPQSTVVAEDQ EWVNVYYEMPDFDVARISPWLLRVELDRKHMTDRKLTMEQIAEKINAGFGDDLNCIFNDDNAEKLVLRIR IMNSDENKMQEEEEVVDKMDDDVFLRCIESNMLTDMTLQGIEQISKVYMHLPQTDNKKKIIITEDGEFKA LQEWILETDGVSLMRVLSEKDVDPVRTTSNDIVEIFTVLGIEAVRKALERELYHVISFDGSYVNYRHLAL LCDTMTCRGHLMAITRHGVNRQDTGPLMKCSFEETVDVLMEAAAHGESDPMKGVSENIMLGQLAPAGTGC FDLLLDAEKCKYGMEIPTNIPGLGAAGPTGMFFGSAPSPMGGISPAMTPWNQGATPAYGAWSPSVGSGMT PGAAGFSPSAASDASGFSPGYSPAWSPTPGSPGSPGPSSPYIPSPGGAMSPSYSPTSPAYEPRSPGGYTP QSPSYSPTSPSYSPTSPSYSPTSPNYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSP TSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPNYSP TSPNYTPTSPSYSPTSPSYSPTSPNYTPTSPNYSPTSPSYSPTSPSYSPTSPSYSPSSPRYTPQSPTYTP SSPSYSPSSPSYSPTSPKYTPTSPSYSPSSPEYTPASPKYSPTSPKYSPTSPKYSPTSPTYSPTTPKYSP TSPTYSPTSPVYTPTSPKYSPTSPTYSPTSPKYSPTSPTYSPTSPKGSTYSPTSPGYSPTSPTYSLTSPA ISPDDSDEEN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001291068 |
ORF Size | 5910 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001291068.1, NP_001277997.1 |
RefSeq Size | 6736 bp |
RefSeq ORF | 5913 bp |
Locus ID | 20020 |
UniProt ID | P08775 |
Cytogenetics | 11 42.86 cM |
MW | 217.6 kDa |
Gene Summary | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates (By similarity). Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing (By similarity). Regulation of gene expression levels depends on the balance between methylation and acetylation levels of tha CTD-lysines (PubMed:26687004). Initiation or early elongation steps of transcription of growth-factors-induced immediate early genes are regulated by the acetylation status of the CTD (PubMed:24207025). Methylation and dimethylation have a repressive effect on target genes expression (PubMed:26687004).[UniProtKB/Swiss-Prot Function] |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC229686 | Polr2a (untagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide A (Polr2a) |
USD 1,822.00 |
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