Kcnt1 (NM_001302351) Mouse Tagged ORF Clone

CAT#: MR231697

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  • TrueORF®

Kcnt1 (myc-DDK-tagged) - Mouse potassium channel, subfamily T, member 1 (Kcnt1), transcript variant 3



  "NM_001302351" in other vectors (1)

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Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 1,172.00


Availability*
6 Weeks

Size
    • 10 ug


Product images

Other products for "Kcnt1"

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Kcnt1
Synonyms C030030G16Rik; s; Sl; Slack; slo2; Slo2.2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR231697 representing NM_001302351
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCGGGCCAAGCTGCCGCGCTCGCCGTCCGAGGGCAAGGCGGGTCCGGGGGACACCCCAGCCGGCG
CTGCAGCCCCTGAGGAGCCACACGGGCTCAGCCCGCTACTGCCGGCCCGCGGCGGGGGCTCCGTGGGCAG
CGACGTGGGCCAGAGGGTCCAGGTGGAGTTCTATGTCAATGAGAACACCTTCAAAGAGCGGCTCAAGCTG
TTCTTCATCAAAAACCAAAGATCCAGCCTGAGGATCCGGCTGTTCAACTTCTCCCTCAAGCTCCTCACCT
GCCTGCTGTATATCGTCCGTGTCCTGCTTGACAACCCAGACCAGGGCATCGGATGCTGGGGCTGCACGAA
GTATAACTACACTTTCAATGGCTCATCCTCTGAGTTCCACTGGGCTCCCATCCTGTGGGTGGAGAGGAAA
ATGGCTCTGTGGGTGATCCAGGTCATTGTGGCCACAATAAGCTTCTTAGAGACCATGCTCATCATTTACC
TCAGCTACAAAGGCAACATCTGGGAGCAGATATTCCATGTGTCTTTCGTCTTGGAGATGATCAACACACT
GCCCTTCATCATCACGGTCTTCTGGCCACCTCTGCGGAACCTGTTCATCCCCGTGTTTCTCAACTGCTGG
CTGGCCAAGCATGCGCTGGAGAACATGATTAATGACTTCCACCGTGCCATCCTACGCACACAGTCAGCCA
TGTTCAACCAGGTGCTCATCCTGTTCTGCACCCTGCTGTGCCTGGTCTTCACAGGGACCTGTGGGATCCA
GCACCTGGAGCGAGCAGGTGGCAACTTGAACCTGCTGACCTCCTTCTACTTCTGCATCGTGACTTTCTCA
ACTGTGGGCTTCGGTGATGTGACGCCCAAGATCTGGCCATCCCAGCTCCTGGTGGTCATCCTGATCTGTG
TCACCCTTGTGGTGCTCCCACTGCAATTTGAAGAGCTTGTCTACCTCTGGATGGAGCGTCAGAAGTCAGG
GGGCAACTATAGCCGCCACCGAGCACGGACGGAGAAGCACGTAGTCCTGTGTGTGAGCTCCCTCAAGATT
GATCTCCTCATGGACTTCCTGAATGAGTTCTATGCCCATCCCCGGCTCCAGGACTACTACGTGGTCATCC
TGTGTCCCTCTGAAATGGACGTCCAGGTGCGCAGGGTGCTGCAGATTCCCCTGTGGTCCCAGCGGGTCAT
CTACCTCCAGGGCTCTGCCCTCAAGGACCAGGATCTCATGCGAGCCAAGATGGACAACGGAGAGGCCTGC
TTTATCCTCAGCAGCAGGAATGAGGTGGACCGCACAGCTGCGGATCACCAGACCATCCTTCGAGCCTGGG
CTGTGAAGGACTTTGCCCCCAACTGTCCCCTCTATGTCCAGATCCTCAAGCCCGAAAACAAGTTTCACGT
CAAATTTGCTGACCACGTGGTATGCGAGGAAGAGTGCAAGTACGCCATGCTGGCCCTGAACTGCATCTGC
CCGGCCACCTCCACCCTCATCACCCTGCTGGTGCACACGTCCCGTGGCCAGGAAGGACAGGAGTCTCCCG
AGCAGTGGCAGCGCACGTACGGGAGGTGCTCGGGCAACGAGGTGTACCACATTCGCATGGGTGACAGTAA
ATTCTTCCGGGAGTATGAGGGCAAGAGCTTCACCTACGCAGCCTTCCACGCGCACAAGAAATATGGGGTG
TGCCTCATCGGGCTGAAGCGTGAGGAGAACAAGAGTATCCTGCTGAACCCAGGACCACGGCACATCCTGG
CTGCCTCCGACACCTGCTTCTATATCAATATTACCAAGGAGGAGAACTCAGCTTTCATCTTCAAACAGGA
GGAGAAGCAGAAGAGACGGGGCCTTGCAGGGCAGGCACTATATGAAGGGCCCTCCCGGCTCCCAGTGCAT
AGCATCATCGCCTCTATGGGGACAGTGGCCATGGACCTGCAGAACACAGATTGCCGGCCCTCCCAGGGTG
GCAGTGGCGGGGACGGCACAAAGCTGACTCTGCCCACCGAGAACGGCTCTGGTAGTCGACGCCCCAGCAT
CGCACCCGTTCTGGAGTTGGCAGACAGCTCAGCCCTGTTGCCCTGCGACCTGCTGAGTGACCAGTCAGAG
GATGAGGTGACACCCTCGGACGACGAGGGGCTCTCTGTGGTTGAGTACGTGAAGGGCTATCCCCCCAACT
CACCCTACATTGGCAGCTCCCCGACTTTATGCCACCTCCTGCCTGTGAAAGCCCCCTTCTGCTGCCTGCG
GTTGGACAAGGGCTGCAAACACAACAGCTACGAGGATGCCAAGGCCTATGGGTTCAAGAACAAGCTGATT
ATTGTCTCTGCTGAGACGGCAGGCAACGGGCTCTACAACTTCATCGTGCCTCTGCGCGCCTACTACCGGT
CCCGCCGGGAGCTCAACCCTATCGTGTTGCTGCTTGACAACAAGCCTGACCACCACTTCCTGGAGGCCAT
CTGTTGCTTCCCCATGGTCTACTACATGGAGGGATCCGTGGACAACCTGGACAGCTTACTGCAGTGTGGC
ATCATCTATGCTGACAACCTGGTGGTGGTGGACAAGGAGAGCACTATGAGCGCTGAAGAGGACTACATGG
CAGATGCCAAGACCATCGTCAATGTGCAAACCATGTTCCGGCTTTTCCCCAGTCTCAGCATCACCACGGA
GCTCACACACCCTTCCAACATGCGGTTCATGCAGTTCCGTGCCAAGGACAGCTACTCTCTGGCTCTTTCC
AAACTTGAAAAGCAAGAACGGGAGAACGGCTCCAACCTGGCCTTCATGTTCCGCCTGCCATTTGCTGCTG
GTCGAGTATTTAGTATCAGCATGTTGGATACACTGCTCTACCAGTCCTTCGTGAAGGACTACATGATCAC
CATCACCAGGCTGCTCTTGGGCCTTGATACTACACCAGGCTCCGGCTACCTCTGTGCAATGAAGGTAACC
GAGGACGACCTGTGGATCCGCACTTACGGCCGCCTCTTCCAGAAACTCTGCTCCTCCAGCGCCGAGATCC
CCATCGGCATCTACAGGACCGAGTGCCATGTCTTCTCGGAGCCCCATGACGTCAGAGCCCAGTCTCAGAT
CTCGGTGAACATGGAGGACTGCGAGGATACTCGGGAGGCCAAGGGACCCTGGGGCACACGAGCTGCATCT
GGCAGTGGCAGCACCCATGGCCGTCACGGGGGCAGTGCTGACCCAGTGGAGCACCCACTACTACGTCGCA
AGAGCCTGCAGTGGGCCCGCAAGCTGAGTCGCAAGAGCACCAAGCAGGCAGGGAAGGCACCTGTGGCCAC
AGACTGGATCACCCAGCAGCGGCTCAGCCTGTACCGACGCTCAGAGCGCCAGGAGCTCTCAGAGCTGGTC
AAGAACCGAATGAAGCACCTGGGACTGCCCACCACTGGCTATGAGGACGTAGCAAATTTAACAGCCAGTG
ATGTGATGAATCGGGTAAACCTGGGATATTTGCAAGATGAGATGAACGATCATCACCAGAACACCCTTTC
CTATGTACTCATCAACCCCCCGCCAGACACAAGACTGGAACCCAACGACATTGTGTACCTCATCCGTTCC
GACCCCCTGGCCCATGTGGCCAGCAGCTCCCAGAGTCGGAAAAGCAGCTGCAGCAACAAGCTCTCATCCT
GTAATCCTGAGACCAGGGATGAGACCCAGCTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR231697 representing NM_001302351
Red=Cloning site Green=Tags(s)

MARAKLPRSPSEGKAGPGDTPAGAAAPEEPHGLSPLLPARGGGSVGSDVGQRVQVEFYVNENTFKERLKL
FFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERK
MALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIPVFLNCW
LAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFS
TVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKI
DLLMDFLNEFYAHPRLQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEAC
FILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCIC
PATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGV
CLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVH
SIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSE
DEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLI
IVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCG
IIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALS
KLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVT
EDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQSQISVNMEDCEDTREAKGPWGTRAAS
GSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELV
KNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRS
DPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001302351
ORF Size 3672 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001302351.1, NP_001289280.1
RefSeq Size 5945 bp
RefSeq ORF 3675 bp
Locus ID 227632
UniProt ID Q6ZPR4
Cytogenetics 2 A3
MW 138.6 kDa
Gene Summary This gene encodes a member of the Slo potassium channel family that has shown to be activated by both sodium and chloride ions. This channel represents the largest potassium channel subunit yet identified. This channel may be important in development and pain signaling. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

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