Cgn (NM_001293727) Mouse Tagged ORF Clone

CAT#: MR231665

Cgn (myc-DDK-tagged) - Mouse cingulin (Cgn), transcript variant 2

Product Images

Circular map for MR231665

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cgn
• Synonyms: 6330408J11Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231665 representing NM_001293727
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGCTGATCCCCGGGGCCCCGTAGACCATGGAGTTCAAATTCGATTTATTACAGAGCCAGAGGGTG
  CTACTGAAATGGGCACCCTTCGTCGTAGTGGGCGGCGCCCTGCAAGGGATGCAAGAGCCAGTACCTATGG
  GGTCGCTGTGCGTGTGCAGGGCATCGCCGGGCAACCGTTTGTGGTTCTTAACAGTGGAGAGAAAGGGACT
  GACTCCTTTGGGGTCCAGATCAAAGGGGGCAACAACCGGGGGTCCCCAGGTGCACTGAGCTCCGATTCAG
  AGCTCCCCGAAAACCCCTACTCTCAAGTCAAGGGACGCCCTGCCACTTCTCGGAGCAGCACATCTGATGA
  GGAGCCCAAGGACCACTTGAATGGAAAACTGATCCGTTCCCAGTCGCAAGCCTCTCTGACCGGGCTTGCC
  TTTATGAGTCCTAGTAATAGGAGCACCAGCTTGCTGGAACTAGCCCCTAAACCGACTTCCTCGATTAACA
  CCATTGACACTGCCCCCTTGTCTTCAGTGGACTCGCTCATCAACAAGTTTGATAGTCAGAAGGGGGGGCA
  GGTCCGGGGTCGCACTGGGCGCCGCACAAGGACACTTCCCCACGAACAGCGCAAGCGGAGCCAGAGTCTG
  GACAGCAGGCTCCCACGGGATACACGTGAGGAACGGGAGCACCAGTCCGCTAACCACTGGACCCGCGGCA
  CAAAGTATGACAACCATGTGGACAGTTCAAAGAACCCATCGCAGAAGCAGAGCCCGTTCAGCAGCTTCAG
  CCGTTCGCGTCAGACTCAAGACTGGGTGCTTCAGAGTTTTGAGGAGACACGGGATCCAGCCATGGTGCAG
  TTCAAATCAACTCCAGACCTTCTCAGAGACCAGAGAGAGACAGCGCCACCAGGCAGTGCGGACCATGTGA
  AGGCCACCATCTATGGCATCCTAAGGGAGGGAAGCTCGGAGAGTGAGGCATCTGTGAGGAGGAAAGTTAG
  TTTGGTGCTGGAGCAAATGCAGCCTCTGGGGGCCCTGGCAGGACAGGCTGAGCTTACCCGAAAAATGGAG
  GAACTTCAGAAGAAGCTGGATGAGGAGGTGAAGAAGCGGCAGAAGCTAGAACCATCCAGGGTTGGGCTGG
  AGAGGCAGCTGGAGGAGAAGGCTGAAGAGTGCCACCGACTGCAGGAGTTGCTGGAGAGGAGAAAGGGGGA
  GGTCCAGCAGAGCAGCAAGGAACTTCAGAACATGAAGCTCCTGCTAGGCCAGGAAGAAGGACTACGGCAT
  GGGCTGGAGGCCCAGGTGAAGGAGCTACAGCTTAAGCTCAAGCATAGCCAGAGTCCCGATTCTGGAAAGG
  AAAGTCTCCTAAAGGACTTGTTAGACACCCGGGAACTTCTAGAAGAACTCTTAGAGGGCAAACAACGAGT
  AGAGGAGCAGCTCAGGCTGAGGGAACGGGAGCTGACAGCCCTGAAGGGGGCCCTGAAAGAGGAGGTGGCC
  TCCCACGACCAGGAGGTGGAACATGTCCGGCTGCAGTACCAGCGAGACACGGAGCAGCTTCGCCGGAGCA
  TGCAAGATGCGACCCAGGACCATGCTGCACTGGAGGCTGAGAGGCAGAAGATGTCTTCCCTCGTGCGGGA
  GCTGCAAAGAGAGCTAGAGGAGACCTCTGAGGAGACAGGGCATTGGCAGAGCATGTTCCAGAAGAACAAG
  GAAGAGCTGAGAGCCACCAAGCAGGAACTGCTGCAACTGCGGATGGAGAAGGAGGAGATGGAAGAGGAAC
  TTGGGGAGAAGATGGAGGTCTTACAGAGGGACTTAGAGCAGGCCCGAGCCAGTACAAGAGATACTCACCA
  GGTTGAGGAACTTAAAAAGGAGCTTCGACGCACCCAGGGGGAGCTTAAAGAGCTTCAGGCAGAGCAGCAG
  AACCAGGAGGTGACTGGGCGACACCAGAACCAGGTGTTAGAGAAGCAGCTGGCAGCGCTGAGGGAGGAGG
  CTGATCGAGGCCGAGAGCTGGAGCAGCAGAACCTCCAGCTACAAAAGACCCTGCAGCAACTGCGGCAGGA
  CTGTGAAGAGGCTTCCAAGGCTAAGGTGGCATCAGAGACAGAGGCCATGGTGCTGGGGCAGCGTCGGGCA
  ACCGTGGAAACCACACTTCGGGAGACACAAGAGGAAAATGACGAATTCCGAAGGCGCATCCTGGGTCTGG
  AGCAGCAGTTGAAGGAGGCCCGAGGTCTGGCGGAAGGTGGGGAAGCGGTGGAGGCACGGCTTCGGGACAA
  AGTGCATCGGCTGGAGGTAGAGAAACAGCAGCTGGAAGAGGCTTTGAATGCTGCCCGGGAGGAGGAGGGG
  AACCTGGCAGCAGCCAAGCGGGCTCTGGAGGTCCGGCTAGATGAGGCCCAGCGGGGGCTGGCACGCCTTG
  GGCAGGAACAGCAAGCACTGAACCGGGCCCTAGAGGAAGAGGGGAAGCAGCGGGAGGCTCTCCGGAGGAG
  CAAGGCAGAGTTGGAGGAGCAGAAGCGTTTGCTGAACAGGACTGTAGACCGACTCAACAAAGAGCTGGAG
  CAGATTGGGGATGACTCCAAGCTAGCCCTGCAGCAGCTCCAGGCTCAGATGGAAGATTACAAGGAAAAGG
  CCCGGAAGGAAGTAGCTGATGCCCAGCGTCAGGCCAAGGATTGGGCCAGTGAGGCTGAAAAGAATTCAGG
  GGGGCTGAGCCGGCTTCAGGATGAGCTGCAAAGGCTGCGGCAAGCCCTGCAGACATCCCAGGCTGAGCGG
  GACACAGCCAGGCTGGACAAAGAGCTGCTGGCCCAGCGACTGCAGGGTCTAGAGCAAGAGGCTGAGAACA
  AGAAACGCTTCCAGGACGACAAGGCTCGACAGCTGAAGAGCCTAGAGGAGAAAGTCTCGCGGCTGGAAGC
  GGAGTTAGATGAAGAAAAGAACACTGTGGAACTGTTAACAGATCGGGTGAACCGTGGCCGGGATCAGGTG
  GATCAACTGAGGACAGAGCTCATGCAGGAGAGATCTGCTCGACAGGACTTGGAGTGTGACAAAATCTCCT
  TGGAGAGACAGAACAAGGATTTGAAGACCCGGTTGGCCAGTTCAGAGGGCTTCCAGAAACCCAGCGCTAG
  CCTCTCCCAGCTTGAGTCCCAGAATCAGCTGCTGCAAGAGCGGCTGCAAGCTGAAGAGAGAGAGAAGACA
  GTTCTGCAGTCCACCAATCGCAAACTAGAACGGAGGGTTAAGGAGCTCTCCATCCAGATTGATGATGAAC
  GGCAGCATGTCAACGACCAGAAGGACCAGCTAACCCTGAGGGTGAAGGCTTTGAAGCGGCAGGTGGATGA
  AGCAGAAGAGGAAATTGAGCGGCTGGACAGCCTGAGGAAGAAGGCACAGCGGGAACTGGAGGAACAGCAT
  GAGGTCAATGAACAGCTCCAAGCCCGGATCAAATCCTTGGAAAAGGATGCCTGGCGGAAAGCTTCCCGCT
  CAGCTGCTGAGTCAGCCCTCAAACAAGAGGGGCTGAGCTCTGATGAGGAGTTTGACAACGTCTACGACCC
  CTCATCCATTGCATCACTGCTTACTGAGAGCAACCTGCAGACTAGCTCATGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231665 representing NM_001293727
  Red=Cloning site Green=Tags(s)
  
  MMADPRGPVDHGVQIRFITEPEGATEMGTLRRSGRRPARDARASTYGVAVRVQGIAGQPFVVLNSGEKGT
  DSFGVQIKGGNNRGSPGALSSDSELPENPYSQVKGRPATSRSSTSDEEPKDHLNGKLIRSQSQASLTGLA
  FMSPSNRSTSLLELAPKPTSSINTIDTAPLSSVDSLINKFDSQKGGQVRGRTGRRTRTLPHEQRKRSQSL
  DSRLPRDTREEREHQSANHWTRGTKYDNHVDSSKNPSQKQSPFSSFSRSRQTQDWVLQSFEETRDPAMVQ
  FKSTPDLLRDQRETAPPGSADHVKATIYGILREGSSESEASVRRKVSLVLEQMQPLGALAGQAELTRKME
  ELQKKLDEEVKKRQKLEPSRVGLERQLEEKAEECHRLQELLERRKGEVQQSSKELQNMKLLLGQEEGLRH
  GLEAQVKELQLKLKHSQSPDSGKESLLKDLLDTRELLEELLEGKQRVEEQLRLRERELTALKGALKEEVA
  SHDQEVEHVRLQYQRDTEQLRRSMQDATQDHAALEAERQKMSSLVRELQRELEETSEETGHWQSMFQKNK
  EELRATKQELLQLRMEKEEMEEELGEKMEVLQRDLEQARASTRDTHQVEELKKELRRTQGELKELQAEQQ
  NQEVTGRHQNQVLEKQLAALREEADRGRELEQQNLQLQKTLQQLRQDCEEASKAKVASETEAMVLGQRRA
  TVETTLRETQEENDEFRRRILGLEQQLKEARGLAEGGEAVEARLRDKVHRLEVEKQQLEEALNAAREEEG
  NLAAAKRALEVRLDEAQRGLARLGQEQQALNRALEEEGKQREALRRSKAELEEQKRLLNRTVDRLNKELE
  QIGDDSKLALQQLQAQMEDYKEKARKEVADAQRQAKDWASEAEKNSGGLSRLQDELQRLRQALQTSQAER
  DTARLDKELLAQRLQGLEQEAENKKRFQDDKARQLKSLEEKVSRLEAELDEEKNTVELLTDRVNRGRDQV
  DQLRTELMQERSARQDLECDKISLERQNKDLKTRLASSEGFQKPSASLSQLESQNQLLQERLQAEEREKT
  VLQSTNRKLERRVKELSIQIDDERQHVNDQKDQLTLRVKALKRQVDEAEEEIERLDSLRKKAQRELEEQH
  EVNEQLQARIKSLEKDAWRKASRSAAESALKQEGLSSDEEFDNVYDPSSIASLLTESNLQTSSC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001293727
• ORF Size: 3552 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001293727.1, NP_001280656.1
• RefSeq Size: 5015 bp
• RefSeq ORF: 3555 bp
• Locus ID: 70737
• Cytogenetics: 3 40.74 cM
• MW: 136.2 kDa