Itgal (NM_001253872) Mouse Tagged ORF Clone

CAT#: MR231638

Itgal (myc-DDK-tagged) - Mouse integrin alpha L (Itgal), transcript variant 1

Product Images

Circular map for MR231638

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Itgal
• Synonyms: (p180); Cd11a; LFA-1; LFA-1A; Ly-15; Ly-21
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231638 representing NM_001253872
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTTTCCGGATTGCGGGCCCCAGACTTTTGCTACTGGGACTCCAGCTGTTTGCCAAGGCCTGGAGCT
  ACAACCTGGACACACGGCCTACGCAGAGCTTCTTGGCACAAGCTGGAAGACATTTTGGGTACCAGGTCTT
  GCAGATTGAAGATGGGGTTGTCGTGGGAGCCCCAGGTGAGGGGGACAACACGGGAGGCCTCTATCACTGC
  CGAACAAGCAGCGAGTTCTGCCAGCCAGTCAGCCTACATGGTTCTAACCATACCTCCAAGTACTTGGGAA
  TGACGCTGGCAACAGATGCCGCCAAGGGAAGCCTTTTGGCCTGTGACCCTGGACTGTCTCGGACATGCGA
  TCAGAACACTTACCTCAGTGGCCTCTGCTACCTCTTCCCCCAGAGTCTGGAGGGACCTATGTTACAAAAT
  CGTCCCGCCTATCAGGAATGTATGAAGGGCAAAGTCGACCTGGTGTTTCTGTTCGATGGCTCACAGAGCT
  TGGATAGAAAGGACTTTGAAAAAATCCTGGAATTCATGAAGGATGTGATGAGGAAGCTCAGCAATACTTC
  CTACCAGTTTGCTGCCGTCCAGTTCTCCACAGACTGCAGAACAGAATTTACTTTCTTGGACTACGTTAAG
  CAGAACAAGAACCCCGATGTTCTGCTAGGCAGCGTGCAGCCCATGTTCTTGCTGACCAATACCTTTCGTG
  CCATCAACTATGTGGTGGCACACGTGTTCAAAGAAGAGTCTGGTGCCAGGCCGGATGCTACCAAGGTGCT
  TGTCATCATTACAGACGGGGAGGCAAGTGATAAAGGCAACATCAGTGCGGCCCACGACATAACCCGCTAC
  ATCATCGGGATTGGCAAGCATTTTGTGAGCGTACAAAAGCAAAAGACGCTCCACATATTTGCCTCAGAAC
  CTGTAGAGGAATTTGTGAAGATTCTGGACACCTTTGAGAAGCTGAAGGATCTTTTTACTGACCTGCAGAG
  GAGGATTTATGCTATTGAGGGCACAAACAGACAGGACCTGACATCCTTTAACATGGAACTCTCCTCCAGC
  GGGATCAGCGCAGACCTCAGCAAGGGCCATGCAGTTGTGGGAGCTGTTGGGGCTAAGGATTGGGCCGGGG
  GCTTTCTGGACCTGCGTGAAGACCTGCAGGGTGCCACATTTGTTGGGCAGGAACCGCTGACCTCAGATGT
  GAGAGGGGGCTACCTGGGTTACACTGTGGCCTGGATGACCTCCCGGAGCTCCAGACCCCTGCTGGCAGCA
  GGAGCCCCACGGTACCAGCATGTGGGACAAGTACTGCTTTTCCAAGCCCCAGAGGCTGGAGGACGTTGGA
  ACCAAACCCAGAAGATAGAAGGGACTCAGATCGGATCTTACTTTGGTGGGGAACTATGTAGTGTTGACCT
  GGACCAAGATGGCGAGGCAGAGCTGCTGCTGATTGGAGCACCCCTGTTCTTTGGGGAGCAGAGAGGAGGC
  CGAGTGTTCACTTACCAGAGAAGACAGTCGCTGTTTGAAATGGTCTCAGAGCTACAGGGTGACCCTGGCT
  ACCCGCTTGGTCGGTTTGGAGCCGCCATAACTGCCCTGACGGACATCAATGGGGATAGGCTGACTGATGT
  GGCTGTGGGAGCCCCTTTGGAGGAGCAGGGGGCTGTGTACATCTTCAATGGGAAGCCTGGTGGGCTCAGT
  CCCCAGCCAAGCCAGCGTATACAAGGAGCCCAGGTGTTCCCAGGAATCCGGTGGTTTGGCCGCTCCATCC
  ATGGGGTGAAGGACCTTGGAGGGGACAGGCTGGCAGATGTGGTTGTAGGAGCTGAGGGTCGGGTGGTTGT
  GCTGAGCTCCAGGCCGGTGGTGGATGTGGTCACTGAGCTGTCGTTCTCCCCAGAGGAAATCCCAGTGCAC
  GAGGTGGAGTGCTCCTACTCAGCCAGGGAGGAGCAGAAACACGGAGTCAAGCTCAAGGCATGCTTCCGGA
  TCAAGCCCCTCACGCCACAGTTTCAAGGTCGCCTGCTTGCCAACCTCAGCTACACCCTGCAGCTGGATGG
  CCATCGGATGAGGAGCCGAGGGTTGTTCCCAGATGGAAGCCACGAGCTCAGTGGAAACACCTCCATCACC
  CCAGATAAATCCTGCTTGGACTTCCACTTCCACTTCCCGATCTGCATTCAAGACCTCATCTCCCCTATCA
  ATGTCTCCCTGAATTTCTCTCTTTTGGAGGAAGAAGGAACACCAAGGGACCAAAAGGTGGGCAGGGCCAT
  GCAGCCTATCCTGAGACCTTCAATCCACACAGTGACTAAGGAGATCCCTTTTGAGAAGAACTGTGGTGAA
  GATAAGAAGTGTGAGGCAAACCTGACCCTGTCATCCCCTGCCAGATCTGGACCCCTGCGTCTGATGTCCT
  CTGCCAGCCTTGCTGTGGAGTGGACACTGAGCAACTCAGGGGAAGATGCCTACTGGGTGCGATTAGACCT
  GGACTTCCCTCGGGGACTCTCCTTCCGGAAAGTGGAGATGCTTCAGCCACACAGCCGAATGCCTGTGAGC
  TGCGAGGAGCTCACCGAGGGGTCAAGTCTCCTGACTAAGACACTGAAATGCAATGTAAGCTCTCCCATCT
  TCAAAGCAGGCCAGGAGGTGAGCCTCCAGGTGATGTTTAACACGCTACTCAACAGCTCCTGGGAAGACTT
  CGTTGAGCTGAATGGCACTGTGCACTGTGAGAATGAGAACTCAAGCCTCCAGGAGGACAACTCAGCCGCC
  ACCCACATTCCTGTCCTGTACCCTGTCAACATCCTTACTAAGGAGCAGGAGAACTCCACTCTCTATATCA
  GTTTCACCCCTAAAGGTCCCAAGACCCAACAAGTCCAGCATGTCTACCAGGTGAGGATTCAGCCATCTGC
  CTATGACCACAACATGCCCACACTAGAGGCCTTGGTTGGGGTGCCCTGGCCTCACAGTGAGGACCCCATC
  ACATACACATGGAGTGTACAGACGGATCCCCTTGTCACTTGCCACAGCGAGGACCTGAAGAGGCCGTCCA
  GCGAAGCTGAGCAGCCTTGTCTGCCTGGAGTCCAGTTCCGCTGTCCAATTGTCTTCAGGCGGGAGATCCT
  CATCCAAGTGACGGGGACCGTGGAACTCTCCAAGGAAATCAAGGCCTCCTCCACACTCAGCCTCTGCAGC
  TCACTCTCCGTCTCCTTCAACAGCAGCAAGCATTTCCATTTGTATGGCAGCAAAGCCTCCGAGGCCCAGG
  TCCTCGTGAAGGTTGACCTGATCCACGAGAAGGAGATGCTTCACGTGTACGTACTCAGCGGCATTGGGGG
  CCTCGTGCTTCTGTTCCTGATTTTCCTGGCGCTCTACAAGGTTGGCTTCTTCAAACGGAACCTGAAGGAG
  AAGATGGAGGCTGATGGAGGTGTTCCAAATGGAAGCCCTCCAGAAGACACTGACCCTCTGGCAGTACCTG
  GGGAAGAGACCAAAGATATGGGCTGTCTAGAGCCCCTCCGGGAGAGTGACAAGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231638 representing NM_001253872
  Red=Cloning site Green=Tags(s)
  
  MSFRIAGPRLLLLGLQLFAKAWSYNLDTRPTQSFLAQAGRHFGYQVLQIEDGVVVGAPGEGDNTGGLYHC
  RTSSEFCQPVSLHGSNHTSKYLGMTLATDAAKGSLLACDPGLSRTCDQNTYLSGLCYLFPQSLEGPMLQN
  RPAYQECMKGKVDLVFLFDGSQSLDRKDFEKILEFMKDVMRKLSNTSYQFAAVQFSTDCRTEFTFLDYVK
  QNKNPDVLLGSVQPMFLLTNTFRAINYVVAHVFKEESGARPDATKVLVIITDGEASDKGNISAAHDITRY
  IIGIGKHFVSVQKQKTLHIFASEPVEEFVKILDTFEKLKDLFTDLQRRIYAIEGTNRQDLTSFNMELSSS
  GISADLSKGHAVVGAVGAKDWAGGFLDLREDLQGATFVGQEPLTSDVRGGYLGYTVAWMTSRSSRPLLAA
  GAPRYQHVGQVLLFQAPEAGGRWNQTQKIEGTQIGSYFGGELCSVDLDQDGEAELLLIGAPLFFGEQRGG
  RVFTYQRRQSLFEMVSELQGDPGYPLGRFGAAITALTDINGDRLTDVAVGAPLEEQGAVYIFNGKPGGLS
  PQPSQRIQGAQVFPGIRWFGRSIHGVKDLGGDRLADVVVGAEGRVVVLSSRPVVDVVTELSFSPEEIPVH
  EVECSYSAREEQKHGVKLKACFRIKPLTPQFQGRLLANLSYTLQLDGHRMRSRGLFPDGSHELSGNTSIT
  PDKSCLDFHFHFPICIQDLISPINVSLNFSLLEEEGTPRDQKVGRAMQPILRPSIHTVTKEIPFEKNCGE
  DKKCEANLTLSSPARSGPLRLMSSASLAVEWTLSNSGEDAYWVRLDLDFPRGLSFRKVEMLQPHSRMPVS
  CEELTEGSSLLTKTLKCNVSSPIFKAGQEVSLQVMFNTLLNSSWEDFVELNGTVHCENENSSLQEDNSAA
  THIPVLYPVNILTKEQENSTLYISFTPKGPKTQQVQHVYQVRIQPSAYDHNMPTLEALVGVPWPHSEDPI
  TYTWSVQTDPLVTCHSEDLKRPSSEAEQPCLPGVQFRCPIVFRREILIQVTGTVELSKEIKASSTLSLCS
  SLSVSFNSSKHFHLYGSKASEAQVLVKVDLIHEKEMLHVYVLSGIGGLVLLFLIFLALYKVGFFKRNLKE
  KMEADGGVPNGSPPEDTDPLAVPGEETKDMGCLEPLRESDKD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001253872
• ORF Size: 3486 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001253872.1, NP_001240801.1
• RefSeq Size: 5225 bp
• RefSeq ORF: 3489 bp
• Locus ID: 16408
• Cytogenetics: 7 69.44 cM
• MW: 128.8 kDa