Radil (NM_001289588) Mouse Tagged ORF Clone

CAT#: MR231576

Radil (myc-DDK-tagged) - Mouse Ras association and DIL domains (Radil), transcript variant 1

Product Images

Circular map for MR231576

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Radil
• Synonyms: AI536456; D930005D10Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231576 representing NM_001289588
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTTATGGGACTCAGTTGATCATGTCTCCTCCCACCAAGAATAAGCTGAAGCGACAGAGCCAGCTCC
  TATCCACAATGCTGTCCCGGACACTGAGCTACAAGTATCGTGACCTGGATTCCACCTTCTGCAGCCTAGG
  TGCCAGTGACGACCCCTCAGAGCTCTCCACGCAGCTCTCGGCCCCCGGGGTCCTCAAGGTGTTTGGGGAC
  AGTGTCTGTACAGGCACCCACTATAAGAGTGTCTTGGCCACTGGCTCCTCCAGTGCCCAGGAGCTGGTGA
  AAGAGGCACTGGAGCGCTATGCCCTGGACCCTGAGTGTGCTGGCCAGTACGTGCTATGTGATGTTGTGGG
  CCAAGCTGGGGACTCTGGGCAGCGGTGGCAGGCCCAGTGCTTCCGAGTATTTGGCGACAATGAGAAGCCC
  CTCTTGATCCAGGAGTTATGGAAACCCCGAGAAGGCTTGTCCCGGAGGTTTGAGCTAAGGAAGAAGTCAG
  ATGTGGAAGAGCTTGCATCGAGGGATGTGGATACCACCACCGCAGGGATAAACGCCCAAGCCCGGAGGCT
  ACAGCGTATCCGTGCCAAGGGAACCCCAGCCCTCACCTCTGAAGCTGCTCAAAGCTCCCCACCGACCAGG
  CTGCGCCGTACAGTCAGCGAGACCAGCCTAAGCCCAGCACCATCGCTACCTGAGGCTGCTCAGAGGCCTG
  AGGAGCCCGTCCCTGAGGCCATGCGTTACTCGCTGTACCAGTGCCCGCACCTGCTGCTGTTGCAGGGCTA
  CAGCCAGCAGCACGACAGCCTGGTGTACGTGCTCAGCAAGGAACGGCACACAGTGGGCCAGCGCACACCG
  TCTAGCAAACCCAGCATCAGCTTGTCTGCCCCAGACATCCTGCCCCTGCACTGTACCATTCGCCGCCATC
  AGTCCCCAGAGGGTGGCCCGGCAGGGACCAGGCTAGTGCTGGAGCCCATCACAGGGGCTTCGGTGTCAGT
  CAACTTCTCAGAAGTGGGAAGGAACCCTGTGGTGCTACAACATGGCGACCTGCTCTCCCTGGGTCTCTAT
  TACCTGCTGCTGTTCAAGGACCCTGGGCAGGCACAGCCACTGCCTGCTTGTGCCCTCGCCCGTCTTGGGG
  CGGCACCACAGAGCTGTAGAATGTGTGGTGCGGTGCTCAGAGCCCGTGGGGCCCCTTCCTTGCCTGCTGC
  TGTGGTCCGGCGGCGGTCACTGCTCCTGGAGTTTGAGCCCGATGTGGAGGACACACTGCTGCAGAGGATC
  ATGACACTGATTGAGCCTGGGGGTGACGACCACAAGCTGACACCTGCCTTCCTCCTCTGCCTCTGCATCC
  AGCACTCCGCCATGCACTTCCAGCCGGGTACCTTCAGGCATCTCCTGCTCAAGATCTCCAAAAGGGTCCG
  AGACACTGTCTGGGAGAAGACCAAAGAACTAGCGGAAAAGCAGGCACAACTCCAGGAGCCCATCTCCTGG
  GCCAGCTTCCCCATGGCCGACCTGGTTCCCGACCTGCAGCACATCCTGTTTTGGATGTCAAACTCCATCG
  AGCTCCTGTACTTCATCCAGCAGAAGTCCCCGCTGTACGTGCAGAGCATGGAGGAGGAGCTGGACGTCAC
  AGGCTCCAAGGAGTCGCTGTTCTCCTGCACACTGACGGCCAGCGAGGAGGCCATGGCTGCCCTGGAGGAG
  GTGGTGCTCTATGCTTTCCAGCAGTGCGTGTATTACCTCTCCAAGTGCTTGTATGTCTGCCTCCCGGCCC
  TCTTGGAATGCCCCCCATTTCAGACAGAGCGCAGGGAGAGCTGGCGCTCAGGTCCAGCTCTGCCCGAGGA
  GCTTCGGCGAGTGGTGTCAGTGTTCCAGGCCACCTTGGACCTCCTGCAGCAGCTGCAGATGCATCCTGAG
  GTGGCCTCCCAGATGCTGGCTTATCTCTTCTTCTTCTCTGGCACACTGCTCCTCAACCAGGTCCTAGACA
  AGGGGCCCTCTCTGAGTTGCTTCCATTGGCCCAGGGGTGTGCAGGTGTGTGCCCGCCTGCAGCAGTTCCT
  GGAGTGGGCCCGGAGTGCTGGCCTTGGAGCACCTGCTGAGCGCTTCTTCCGGAAGCTTTCCTGCACCCTG
  CATCTGCTGGCCACTCCCAGGGCTCAGCTCATCCAGATGAGCTGGGCCACCCTGAGGGTCACATTCCCTG
  CCCTGAACCCTGCTCAGCTGCACCGGCTTCTGACTCAGTACCAGCTGGCCTCTGCCATGGGCCCTGTGAG
  CGCTTGGGAGCCAGGAGCCCCAGACGGCCCGGAGGCCTTCCAGTCAGAGGACATCCTGGAGTCCTATGAA
  AACCCGCCGCCCATTGTTCTGCCGAGCCAGGGCTTCCAGGTAGACCTGGAAGCCGACTGCGTGGAGGACA
  GCATCTACCAGCACCTGCTCTACATCCGCCACTTCCTGTGGGGGCTGCGGGGCCAAGCCAGCCCTGACAG
  TGGGCCTGCCCAGCCCGAGAGCATCGAGGGAGCCATCATGGATACCTGGGCCATCCCCTCCTCTGGGCCT
  CAACGTCTGTCCACCTTCTCTTCCCAGGGCCTGTACCACACAATTCCTGAGGGCCATCTGGAAGGCCATG
  GCTGCCCCCTGGCCAATAGGGACCCAGGCAGAGTTGCTGTTGAAACTGCCCCTCCCCATTCTCTTCCTGT
  CACGGGAGCTCCCCGGGCTCAGGGTCCCCCTGGAAGACAGCCTACCCGAGGGGACCGCAGGGGCTCCCAG
  GCTGGCTCTCTGCACACTGACTCCTCCTGCATGCTCACTCCGCCCAGCACGCCACTGGGCCTGGAGCCTG
  CAGGCCCCAGCTGGCCAGAGCCCAGCGGCCTCTGTGGAAGAGCAGTCCTTGACGGGCAGAGGAATGGGCC
  AGGTGGTCTGCCTGGTGCAGTCCTAGAAGGAGATGCGATTCAGGATGCTGAGCCTCCTGCTGAGGCCTCC
  AGCCCCAGCTCCAGCGCTGAGGACTTCTGCTACGTGTTCATGGTGGAGCTGGAGCGAGGCCCCTCGGGGC
  TGGGAATGGGCCTGATTGATGGGATGCACACACCTCTGGGGGCCCAGGGACTCTACATACAGACCCTGCT
  TCCCGGGAGCCCCGCGGCATCTGATGGGCGGCTGTCACTCGGAGACCAAATCCTGGAGGTGAATGGCAGC
  AGCCTGAGGGGTGTCAGCTACATGAGGGCAGTGGATTTGATCCGACATGGAGGCAAGAAGATGCGATTTT
  TAGTTGCCAAGTCTGACATGGAAACGGCCAAGAAAATCCGCTTCCGCAATCCCCCTTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231576 representing NM_001289588
  Red=Cloning site Green=Tags(s)
  
  MFYGTQLIMSPPTKNKLKRQSQLLSTMLSRTLSYKYRDLDSTFCSLGASDDPSELSTQLSAPGVLKVFGD
  SVCTGTHYKSVLATGSSSAQELVKEALERYALDPECAGQYVLCDVVGQAGDSGQRWQAQCFRVFGDNEKP
  LLIQELWKPREGLSRRFELRKKSDVEELASRDVDTTTAGINAQARRLQRIRAKGTPALTSEAAQSSPPTR
  LRRTVSETSLSPAPSLPEAAQRPEEPVPEAMRYSLYQCPHLLLLQGYSQQHDSLVYVLSKERHTVGQRTP
  SSKPSISLSAPDILPLHCTIRRHQSPEGGPAGTRLVLEPITGASVSVNFSEVGRNPVVLQHGDLLSLGLY
  YLLLFKDPGQAQPLPACALARLGAAPQSCRMCGAVLRARGAPSLPAAVVRRRSLLLEFEPDVEDTLLQRI
  MTLIEPGGDDHKLTPAFLLCLCIQHSAMHFQPGTFRHLLLKISKRVRDTVWEKTKELAEKQAQLQEPISW
  ASFPMADLVPDLQHILFWMSNSIELLYFIQQKSPLYVQSMEEELDVTGSKESLFSCTLTASEEAMAALEE
  VVLYAFQQCVYYLSKCLYVCLPALLECPPFQTERRESWRSGPALPEELRRVVSVFQATLDLLQQLQMHPE
  VASQMLAYLFFFSGTLLLNQVLDKGPSLSCFHWPRGVQVCARLQQFLEWARSAGLGAPAERFFRKLSCTL
  HLLATPRAQLIQMSWATLRVTFPALNPAQLHRLLTQYQLASAMGPVSAWEPGAPDGPEAFQSEDILESYE
  NPPPIVLPSQGFQVDLEADCVEDSIYQHLLYIRHFLWGLRGQASPDSGPAQPESIEGAIMDTWAIPSSGP
  QRLSTFSSQGLYHTIPEGHLEGHGCPLANRDPGRVAVETAPPHSLPVTGAPRAQGPPGRQPTRGDRRGSQ
  AGSLHTDSSCMLTPPSTPLGLEPAGPSWPEPSGLCGRAVLDGQRNGPGGLPGAVLEGDAIQDAEPPAEAS
  SPSSSAEDFCYVFMVELERGPSGLGMGLIDGMHTPLGAQGLYIQTLLPGSPAASDGRLSLGDQILEVNGS
  SLRGVSYMRAVDLIRHGGKKMRFLVAKSDMETAKKIRFRNPPS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001289588
• ORF Size: 3279 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289588.1, NP_001276517.1
• RefSeq Size: 3796 bp
• RefSeq ORF: 3282 bp
• Locus ID: 231858
• Cytogenetics: 5 G2
• MW: 120.1 kDa
• Gene Summary: Downstream effector of Rap required for cell adhesion and migration of neural crest precursors during development.[UniProtKB/Swiss-Prot Function]