Taok3 (NM_183306) Mouse Tagged ORF Clone

CAT#: MR231349

Taok3 (myc-DDK-tagged) - Mouse TAO kinase 3 (Taok3), transcript variant 2

Product Images

Circular map for MR231349

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Taok3
• Synonyms: 2900006A08Rik; A130052D22; A430105I05Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231349 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGTAAAGGGGCACTGAAGGACCCAGAGATTGCTGATCTGTTCTTCAAGGATGACCCCGAAGAGCTTT
  TTATCGATTTGCATGAAATTGGACATGGGAGCTTTGGAGCCGTGTACTTTGCGACAAATGCGCACACCAA
  CGAGGTGGTCGCTGTTAAGAAAATGTCCTACAGTGGGAAGCAGACCCATGAGAAATGGCAAGATATCCTT
  AAGGAAGTTAAGTTTCTGCAGCAGCTGAAGCATCCTAACACCATCGAGTACAAAGGCTGTTATTTAAAGG
  AGCACACTGCATGGTTGGTGATGGAGTACTGCCTAGGTTCAGCCTCTGACTTGTTAGAAGTTCATAAGAA
  ACCACTTCAGGAAGTGGAGATCGCTGCTATCACCCATGGTGCCTTGCAGGGGCTGGCCTACCTCCACTTT
  CACTCCCTGATTCACAGGGACATTAAAGCAGGGAACATCCTCCTCACAGAGCCAGGTCAGGTGAAGCTGG
  CTGACTTCGGATCTGCCTCCATGGCTTCTCCCGCCAACTCCTTTGTGGGGACTCCTTACTGGATGGCCCC
  AGAGGTAATCTTAGCTATGGACGAAGGGCAGTACGACGGGAAAGTTGACATCTGGTCGCTTGGCATCACC
  TGCATAGAGTTGGCGGAGCGGAAGCCCCCCCTCTTCAACATGAACGCCATGAGCGCCCTCTACCACATCG
  CTCAGAATGACTCCCCCACGCTACAGTCCAGGGAATGGACAGACTCCTTCAGGAGATTCGTTGATTACTG
  CTTGCACAAAATACCTCAGGAAAGACCAGCCGCGGTGGAGCTGTTGCGGCATGACTTCATTCGGAGGGAG
  CGGCCACCGAAGGTCCTCATTGATCTCATACAGAGGACAAAAGATGCTGTCCGAGAGCTGGACAACCTGC
  AGTACAGGAAAATGAAGAAGATCCTCTTCCAGGAGACTCGGAACGGACCCTTGAATGAGTCGCAAGAAGA
  AGAAGAAGATGGTGAGCAAGGAAGCAACCTGAACCGAGAGGTGGACAGTCTGGGCAGCATCCACTCCATT
  CCCAGCACATCAGTGAGCACAGGCAGCCGGAGCAGCAGTGTGAACAGCATGCAGGAGGTCATGGATGAGA
  GCAGCTCTGAGCTCGTCATGATGCAGGAGGATGAAGGCACAGCCAACTCCAGCGCCTCCACGGTACACAA
  GAAGGATCATGTATTCGTAAGGGATGAGGCGGGCCACGGCGATCCCAGGCCTGAGCCGCGGCCTACCCAG
  TCAGTTCAGAGCCGGGCCCTCCACTATCGGAACCGAGAGCGCTTTGCCACGATCAAATCCGCGTCTTTGG
  TTACACGGCAGATCCATGAGCATGAGCAGGAGAACGAGCTGCGGGAACAGATGTCAGGTTATAAGCGGAT
  GCGGCGCCAGCACCAGAAGCAGCTGATCGCCCTGGAGAACAAGCTGAAGGCTGAGATGGATGAGCACCGC
  CTCAAGCTCCAGAAGGAGGTGGAGACGCACGCCAACAACTCGTCCATCGAGCTGGAGAAGCTGGCCAAGA
  AGCAAGTGGCTACCATAGAAAAGGAGGCAAAGGTAGCCGCAGCGGATGAGAAGAAGTTCCAGCAGCAGAT
  CCTGGCCCAGCAGAAGAAGGACTTGACAACTTTCTTAGAAAGTCAGAAGAAGCAGTACAAGATTTGTAAG
  GAGAAAATAAAAGAGGAAATGAACGAGGACCATAGCACACCCAAGAAGGAGAAGCAGGAGCGGATATCGA
  AGCATAAAGAAAACCTGCAGCACACGCAGGCCGAGGAGGAAGCCCACCTGCTCACTCAACAGAGGCTGTA
  CTACGACAGAAACTGCCGCTTCTTCAAACGGAAAATAATGATCAAGCGCCACGAGGTGGAACAGCAGAAC
  ATTCGGGAGGAATTAAATAAGAAGAGGACCCAGAAGGAAATGGAGCACGCCATGCTGATCCGGCATGATG
  AATCCACCCGAGAGCTGGAGTACAGGCAGCTGCACACCCTGCAGAAGCTCCGCATGGATCTGATCCGGCT
  GCAGCACCAGACGGAACTGGAGAACCAGCTGGAGTACAATAAGAGGCGGGAGCGGGAGCTGCACCGGAAG
  CACGTCATGGAGCTTCGGCAACAGCCGAAAAACTTAAAGGCCATGGAAATGCAAATTAAAAAACAGTTTC
  AGGACACTTGCAAAGTACAGACCAAACAGTACAAAGCCCTCAAAAATCACCAGTTGGAAGTCACTCCAAA
  GAATGAGCACAAAGCAATCCTAAAGACACTGAAGGACGAGCAGACGAGGAAGCTGGCCATTTTGGCCGAG
  CAGTATGAACAGAGTATAAACGAGATGATGGCCTCTCAAGCGTTACGGCTAGACGAGGCACAAGAGGCAG
  AGTGCCAGGCCTTGAGACTACAGCTCCAGCAGGAGATGGAGCTGCTCAACGCCTACCAGAGCAAAATCAA
  GATGCAAACGGAGGCCCAGCACGAGCGCGAGCTACAGAAGCTAGAGCAGAGGGTGTCCCTGCGCAGAGCG
  CATCTTGAGCAGAAGATTGAAGAGGAGCTGGCTGCCCTGCAGAAGGAACGCAGTGAGAGGATCAAGACTC
  TCCTGGAGAGACAAGAGCGAGAGACCGAGACTTTTGACATGGAGAGCCTCAGAATGGGCTTTGGGAATTT
  GGTGACATTAGATTTTCCTAAGGAGGACTATAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231349 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRKGALKDPEIADLFFKDDPEELFIDLHEIGHGSFGAVYFATNAHTNEVVAVKKMSYSGKQTHEKWQDIL
  KEVKFLQQLKHPNTIEYKGCYLKEHTAWLVMEYCLGSASDLLEVHKKPLQEVEIAAITHGALQGLAYLHF
  HSLIHRDIKAGNILLTEPGQVKLADFGSASMASPANSFVGTPYWMAPEVILAMDEGQYDGKVDIWSLGIT
  CIELAERKPPLFNMNAMSALYHIAQNDSPTLQSREWTDSFRRFVDYCLHKIPQERPAAVELLRHDFIRRE
  RPPKVLIDLIQRTKDAVRELDNLQYRKMKKILFQETRNGPLNESQEEEEDGEQGSNLNREVDSLGSIHSI
  PSTSVSTGSRSSSVNSMQEVMDESSSELVMMQEDEGTANSSASTVHKKDHVFVRDEAGHGDPRPEPRPTQ
  SVQSRALHYRNRERFATIKSASLVTRQIHEHEQENELREQMSGYKRMRRQHQKQLIALENKLKAEMDEHR
  LKLQKEVETHANNSSIELEKLAKKQVATIEKEAKVAAADEKKFQQQILAQQKKDLTTFLESQKKQYKICK
  EKIKEEMNEDHSTPKKEKQERISKHKENLQHTQAEEEAHLLTQQRLYYDRNCRFFKRKIMIKRHEVEQQN
  IREELNKKRTQKEMEHAMLIRHDESTRELEYRQLHTLQKLRMDLIRLQHQTELENQLEYNKRRERELHRK
  HVMELRQQPKNLKAMEMQIKKQFQDTCKVQTKQYKALKNHQLEVTPKNEHKAILKTLKDEQTRKLAILAE
  QYEQSINEMMASQALRLDEAQEAECQALRLQLQQEMELLNAYQSKIKMQTEAQHERELQKLEQRVSLRRA
  HLEQKIEEELAALQKERSERIKTLLERQERETETFDMESLRMGFGNLVTLDFPKEDYR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_183306
• ORF Size: 2694 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_183306.2, NP_899129.2
• RefSeq Size: 4343 bp
• RefSeq ORF: 2697 bp
• Locus ID: 330177
• UniProt ID: Q8BYC6
• Cytogenetics: 5 F
• MW: 105.3 kDa
• Gene Summary: Serine/threonine-protein kinase that acts as a regulator of the p38/MAPK14 stress-activated MAPK cascade and of the MAPK8/JNK cascade. Acts as an activator of the p38/MAPK14 stress-activated MAPK cascade. In response to DNA damage, involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of upstream MAP2K3 and MAP2K6 kinases. Inhibits basal activity of MAPK8/JNK cascade and diminishes its activation in response epidermal growth factor (EGF) (By similarity).[UniProtKB/Swiss-Prot Function]