Tfr2 (NM_001289507) Mouse Tagged ORF Clone

CAT#: MR231243

Tfr2 (myc-DDK-tagged) - Mouse transferrin receptor 2 (Tfr2), transcript variant 1

Product Images

Circular map for MR231243

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tfr2
• Synonyms: Trfr2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231243 representing NM_001289507
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAAACGGCCTAGGTTCAGGCTGCCTGTCAGGGCTGATAAGGGGCCCTCTAGGGCTCCCACAAACGG
  TTTATCGGGGTTTTGTGTGTGTGTGTGTGGACTACTTGCAGAGTTCAGGAGGGGGCACAAGCATGGAGCA
  ACGTTGGGGTCTACTTCGGAGAGTGCAACAGTGGTCCCCAAGACCCTCTCAGACCATCTACAGACGCGTG
  GAAGGCCCTCAGCTGGAGCACCTGGAGGAGGAAGACAGGGAGGAAGGGGCGGAGCTTCCTGCCCAGTTCT
  GCCCCATGGAACTCAAAGGCCCTGAGCACTTAGGCTCCTGTCCCGGGAGGTCAATTCCCATACCCTGGGC
  TGCAGCAGGTCGAAAGGCTGCCCCCTATCTGGTCCTGATCACCCTGCTAATCTTCACTGGGGCCTTCCTC
  CTAGGCTACGTGGCCTTTCGAGGGTCCTGCCAGGCGTGTGGGGACTCCGTGTTGGTGGTCGATGAAGATG
  TCAACCCTGAGGACTCCGGCCGGACCACGTTGTACTGGAGCGACCTCCAGGCCATGTTTCTCCGGTTCCT
  TGGGGAGGGGCGCATGGAAGACACCATCAGGCTGACCAGCCTCCGGGAACGCGTGGCTGGCTCAGCCAGA
  ATGGCCACCCTGGTCCAAGATATCCTCGATAAGCTCTCGCGCCAGAAGCTGGACCACGTGTGGACTGACA
  CGCACTACGTGGGACTTCAGTTCCCAGATCCGGCTCACGCTAACACCCTGCACTGGGTGGATGCAGACGG
  GAGCGTCCAGGAGCAGCTACCGCTGGAGGATCCGGAAGTCTACTGTCCCTACAGCGCCACCGGCAACGCC
  ACGGGCAAGCTGGTGTACGCCCACTACGGGCGGTCGGAGGACCTACAGGACCTAAAAGCCAAGGGCGTGG
  AGCTGGCCGGCAGCCTCCTGCTAGTGCGAGTTGGAATTACTAGCTTCGCCCAGAAGGTAGCCGTTGCCCA
  GGACTTTGGGGCTCAAGGAGTGCTGATATACCCTGACCCATCAGACTTCTCCCAGGATCCCCACAAGCCA
  GGCCTGTCTAGCCACCAGGCTGTGTACGGACATGTGCACCTGGGAACTGGAGACCCTTACACACCTGGCT
  TCCCGTCCTTCAATCAAACCCAGTTCCCTCCAGTAGAATCATCAGGCCTTCCCAGCATCCCCGCCCAGCC
  CATCAGTGCTGACATTGCTGATCAATTGCTCAGGAAACTCACAGGCCCCGTGGCTCCCCAGGAGTGGAAA
  GGTCACCTCTCAGGCTCTCCTTATCGGCTGGGACCTGGGCCCGACTTACGCCTTGTGGTCAACAACCACA
  GAGTCTCTACCCCCATCAGTAACATCTTTGCGTGCATCGAGGGCTTTGCAGAGCCAGATCACTATGTTGT
  CATTGGGGCCCAGAGGGATGCATGGGGCCCAGGAGCAGCCAAGTCTGCAGTGGGGACTGCCATCCTGCTG
  GAGCTGGTTCGGACCTTCTCTTCCATGGTCAGCAATGGGTTCAGACCTCGAAGAAGTCTTTTGTTCATCA
  GCTGGGACGGAGGTGACTTTGGCAGCGTGGGAGCCACAGAGTGGTTGGAGGGCTACCTCAGCGTGCTACA
  CCTCAAAGCTGTTGTGTACGTGAGCCTGGACAACTCCGTGTTGGGAGATGGCAAATTCCATGCTAAGACC
  AGCCCCCTTCTCGTCAGCCTCATTGAGAATATCTTGAAGCAGGTGGACTCCCCTAACCATAGTGGACAGA
  CCCTCTATGAACAAGTGGCACTCACCCACCCCAGCTGGGATGCTGAAGTGATTCAGCCCCTGCCCATGGA
  CAGCAGTGCATATTCCTTCACAGCCTTTGCGGGGGTCCCAGCTGTGGAGTTCTCCTTCATGGAGGATGAT
  CGGGTGTACCCATTCCTGCACACGAAGGAGGACACATATGAGAATCTGCACAAGATGCTGCGAGGTCGCC
  TGCCCGCCGTGGTCCAGGCAGTGGCTCAGCTCGCGGGCCAGCTCCTCATCCGACTGAGCCACGATCACCT
  ACTGCCGCTAGACTTCGGCCGCTATGGAGACGTGGTTCTCAGGCACATCGGCAACCTCAATGAGTTCTCT
  GGGGACCTCAAGGAGCGCGGGCTGACCCTGCAGTGGGTGTACTCTGCAAGGGGGGACTACATCCGTGCGG
  CGGAAAAGCTGCGGAAGGAGATTTACAGCTCGGAGCGGAACGATGAGCGTCTGATGCGCATGTACAACGT
  GCGCATCATGAGGGTGGAGTTCTACTTCCTGTCCCAGTATGTGTCGCCAGCCGACTCCCCATTCCGCCAC
  ATTTTCCTAGGCCAAGGCGACCACACTTTGGGTGCCCTGGTAGACCACCTGCGGATGCTGCGCGCCGATG
  GCTCAGGAGCCGCCTCTTCCCGGTTGACAGCAGGTCTGGGCTTCCAGGAGAGTCGCTTCCGGCGCCAGCT
  GGCGCTGCTCACCTGGACACTGCAGGGGGCAGCCAACGCTCTCAGTGGCGACGTTTGGAACATTGACAAT
  AACTTT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231243 representing NM_001289507
  Red=Cloning site Green=Tags(s)
  
  MRNGLGSGCLSGLIRGPLGLPQTVYRGFVCVCVDYLQSSGGGTSMEQRWGLLRRVQQWSPRPSQTIYRRV
  EGPQLEHLEEEDREEGAELPAQFCPMELKGPEHLGSCPGRSIPIPWAAAGRKAAPYLVLITLLIFTGAFL
  LGYVAFRGSCQACGDSVLVVDEDVNPEDSGRTTLYWSDLQAMFLRFLGEGRMEDTIRLTSLRERVAGSAR
  MATLVQDILDKLSRQKLDHVWTDTHYVGLQFPDPAHANTLHWVDADGSVQEQLPLEDPEVYCPYSATGNA
  TGKLVYAHYGRSEDLQDLKAKGVELAGSLLLVRVGITSFAQKVAVAQDFGAQGVLIYPDPSDFSQDPHKP
  GLSSHQAVYGHVHLGTGDPYTPGFPSFNQTQFPPVESSGLPSIPAQPISADIADQLLRKLTGPVAPQEWK
  GHLSGSPYRLGPGPDLRLVVNNHRVSTPISNIFACIEGFAEPDHYVVIGAQRDAWGPGAAKSAVGTAILL
  ELVRTFSSMVSNGFRPRRSLLFISWDGGDFGSVGATEWLEGYLSVLHLKAVVYVSLDNSVLGDGKFHAKT
  SPLLVSLIENILKQVDSPNHSGQTLYEQVALTHPSWDAEVIQPLPMDSSAYSFTAFAGVPAVEFSFMEDD
  RVYPFLHTKEDTYENLHKMLRGRLPAVVQAVAQLAGQLLIRLSHDHLLPLDFGRYGDVVLRHIGNLNEFS
  GDLKERGLTLQWVYSARGDYIRAAEKLRKEIYSSERNDERLMRMYNVRIMRVEFYFLSQYVSPADSPFRH
  IFLGQGDHTLGALVDHLRMLRADGSGAASSRLTAGLGFQESRFRRQLALLTWTLQGAANALSGDVWNIDN
  NF
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001289507
• ORF Size: 2526 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289507.1, NP_001276436.1
• RefSeq Size: 3578 bp
• RefSeq ORF: 2529 bp
• Locus ID: 50765
• UniProt ID: Q9JKX3
• Cytogenetics: 5 G2
• MW: 93.3 kDa
• Gene Summary: Mediates cellular uptake of transferrin-bound iron in a non-iron dependent manner. May be involved in iron metabolism, hepatocyte function and erythrocyte differentiation.[UniProtKB/Swiss-Prot Function]