Trim9 (NM_001286387) Mouse Tagged ORF Clone

CAT#: MR231144

Trim9 (myc-DDK-tagged) - Mouse tripartite motif-containing 9 (Trim9), transcript variant 5

Product Images

Circular map for MR231144

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Trim9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231144 representing NM_001286387
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGAGATGGAAGAAGAGTTAAAATGCCCTGTGTGTGGCTCCTTCTATCGGGAGCCCATCATCTTGC
  CCTGCTCTCACAATTTATGTCAGGCGTGCGCCCGCAACATCCTGGTGCAGACCCCGGAGTCCGAGTCCCC
  CCAGAGCCGCCGGGCCTCGGGCTCTGGGGTTTCTGACTATGATTATCTGGACCTGGACAAGATGAGCCTG
  TACAGCGAGGCGGACAGCGGCTATGGCTCCTACGGAGGTTTCGCCAGCGCCCCCACTACCCCGTGCCAGA
  AGTCGCCCAACGGCGTCCGCGTTTTCCCCCCTGCTATGCCGCCACCGCCCACCCACCTGTCACCGGCTTT
  GGCCCCTGTGCCCCGCAATTCCTGCATCACCTGCCCCCAGTGCCACCGCAGCCTCATTCTGGATGACCGG
  GGGCTCCGCGGTTTCCCCAAGAACCGCGTCCTGGAAGGGGTCATCGACCGCTACCAACAGAGCAAAGCCG
  CGGCCCTCAAGTGCCAGCTCTGCGAGAAGGCGCCCAAGGAAGCCACGGTCATGTGTGAACAGTGCGATGT
  CTTCTACTGCGACCCTTGCCGTCTGCGCTGCCACCCGCCCCGGGGTCCCCTAGCCAAACACCGTCTGGTG
  CCCCCGGCCCAGGGCCGCGTCAGCCGGCGGCTGAGCCCGCGCAAGGTCTCCACCTGCACAGACCACGAGC
  TGGAGAACCACAGCATGTACTGCGTGCAATGCAAGATGCCCGTGTGCTACCAGTGCCTAGAGGAGGGCAA
  ACACTCCAGCCACGAAGTCAAGGCTTTGGGGGCTATGTGGAAATTGCACAAGAGCCAGCTCTCCCAGGCC
  CTGAATGGATTGTCGGACAGGGCCAAAGAAGCCAAGGAGTTCCTGGTGCAGCTCCGCACCATGGTACAAC
  AGATCCAGGAAAACAGTGTGGAGTTTGAGGCCTGCCTGGTGGCCCAGTGTGATGCGCTCATCGATGCCCT
  AAACCGAAGGAAGGCTCAGCTGCTGGCCCGGGTCAACAAGGAGCATGAACACAAGCTGAAGGTGGTTCGG
  GACCAGATCTCTCACTGCACGGTGAAGCTGCGTCAGACCACGGGCCTCATGGAGTACTGCTTGGAAGTGA
  TTAAGGAGAACGACCCCAGTGGCTTTTTGCAGATTTCAGATGCTCTCATAAGGCGAGTTCACTTAACTGA
  GGACCAGTGGGGGAAGGGCACCCTCACTCCCAGGATGACCACAGACTTTGATCTGAGCCTGGACAACAGC
  CCTCTGCTGCAGTCTATTCACCAACTGGACTTCGTGCAAGTGAAAGCCTCCTCTCCAGTCCCAGCAACCC
  CCATCCTACAGCTGGAGGAGTGTTGCACCCACAACAACAGTGCTACGCTGTCCTGGAAACAGCCCCCTCT
  GTCCACCGTAGCCGCCGATGGATACATCCTGGAGCTGGATGACGGCAGTGGTGGTCAGTTCCGGGAAGTG
  TATGTTGGAAAAGAAACAATGTGCACAGTGGACGGCCTTCACTTCAACAGCACATACAACGCTCGGGTTA
  AGGCCTTCAACAAAACAGGAGTCAGCCCCTACAGCAAGACACTGGTCCTCCAGACGTCTGAGGCGGGAGC
  CCATGAGACAAAACCTATGAAGGACACAGACTCAGAAGAACAGACCCTCCCCTTTCCAGTGCCTTCGGAA
  AGATTGCCGCTCCGTAGGATGAGCCCTTTCTCCTCCACCCTTAATCTACAACCCAGCTTCCCCGGGAGGT
  CCTACTTTGATTTCCGGTCCTCACCCCACCAGCTGAGTTTGCATTCTTCTTTACAGTCTCTCAATGCACC
  GGGCTGCAATTTTGAGACACAATCTGCATCCTACTCTCAATTAGTTGACATTAAAAAGCTGTTGGCAGTG
  GCTTGGTTTGCTTTTGACCCCGGCTCGGCCCACTCCGACATCATCTTCTCCAATGACAACTTGACAGTGA
  CGTGCAGTAGCTACGATGACCGGGTGGTGCTGGGGAAAACCGGCTTCTCCAAAGGCGTCCACTACTGGGA
  GCTGACCATAGATCGCTATGACAACCACCCTGATCCAGCATTTGGCGTGGCTCGCATCGATGTGATGAAG
  GATATGATGTTAGGGAAGGACGACAAAGCTTGGGCAATAACTGAAGGAGGGATCACAAAAGGGGCCACCA
  TCGGGGTCCTCCTCGACTTAAACAGAAAGACATTAACGTTTTTCGTCAACAATGAACAGCAAGGCCCCAT
  AGCGTTTGAGAACGTGGAGGGCCTGTTCTTCCCTGCTGTCAGCCTGAACAGGAATGTGCAGGTCACGCTC
  CACACCGGGCTTCCGGTCCCCGACTTCTACTCCAGCCGAGCTTCCATAGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231144 representing NM_001286387
  Red=Cloning site Green=Tags(s)
  
  MEEMEEELKCPVCGSFYREPIILPCSHNLCQACARNILVQTPESESPQSRRASGSGVSDYDYLDLDKMSL
  YSEADSGYGSYGGFASAPTTPCQKSPNGVRVFPPAMPPPPTHLSPALAPVPRNSCITCPQCHRSLILDDR
  GLRGFPKNRVLEGVIDRYQQSKAAALKCQLCEKAPKEATVMCEQCDVFYCDPCRLRCHPPRGPLAKHRLV
  PPAQGRVSRRLSPRKVSTCTDHELENHSMYCVQCKMPVCYQCLEEGKHSSHEVKALGAMWKLHKSQLSQA
  LNGLSDRAKEAKEFLVQLRTMVQQIQENSVEFEACLVAQCDALIDALNRRKAQLLARVNKEHEHKLKVVR
  DQISHCTVKLRQTTGLMEYCLEVIKENDPSGFLQISDALIRRVHLTEDQWGKGTLTPRMTTDFDLSLDNS
  PLLQSIHQLDFVQVKASSPVPATPILQLEECCTHNNSATLSWKQPPLSTVAADGYILELDDGSGGQFREV
  YVGKETMCTVDGLHFNSTYNARVKAFNKTGVSPYSKTLVLQTSEAGAHETKPMKDTDSEEQTLPFPVPSE
  RLPLRRMSPFSSTLNLQPSFPGRSYFDFRSSPHQLSLHSSLQSLNAPGCNFETQSASYSQLVDIKKLLAV
  AWFAFDPGSAHSDIIFSNDNLTVTCSSYDDRVVLGKTGFSKGVHYWELTIDRYDNHPDPAFGVARIDVMK
  DMMLGKDDKAWAITEGGITKGATIGVLLDLNRKTLTFFVNNEQQGPIAFENVEGLFFPAVSLNRNVQVTL
  HTGLPVPDFYSSRASIA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286387
• ORF Size: 2361 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286387.1, NP_001273316.1
• RefSeq Size: 4677 bp
• RefSeq ORF: 2364 bp
• Locus ID: 94090
• UniProt ID: Q8C7M3
• Cytogenetics: 12 C2
• MW: 87.9 kDa
• Gene Summary: E3 ubiquitin-protein ligase which ubiquitinates itself in cooperation with an E2 enzyme UBE2D2/UBC4 and serves as a targeting signal for proteasomal degradation. May play a role in regulation of neuronal functions. May act as a regulator of synaptic vesicle exocytosis by controlling the availability of SNAP25 for the SNARE complex formation.[UniProtKB/Swiss-Prot Function]