Sh2b1 (NM_001289541) Mouse Tagged ORF Clone

CAT#: MR230985

Sh2b1 (myc-DDK-tagged) - Mouse SH2B adaptor protein 1 (Sh2b1), transcript variant 5

Product Images

Circular map for MR230985

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sh2b1
• Synonyms: AI425885; C530001K22Rik; Irip; mKIAA1299; Psm; SH2-B; SH2-Bb; Sh2bpsm1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230985 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGGTGCCCCTTCCCCAGAGGATGGGGTCTTCCCTTCTCCGCCAGCGCTGCCACCACCCCCTCCCC
  CAAGTTGGCAAGAGTTCTGTGAGTCCCATGCGAGGGCAGCTGCCCTGGATCTTGCCCGACGTTTTCGCCT
  CTACCTGGCCTCCCACCCACAGTATGCAGAGCCGGGAGCAGAGGCCGCTTTTTCTGGCCGTTTTGCTGAG
  CTCTTCCTGCAGCACTTCGAAGCCGAGGTGGCTCGGGCCTCGGGCTCACTCTCCCCACCTGTCCTGGCTC
  CATTGAGCCCTGGTGTGGAAATCCCACCATCACACGACCTGTCCCTTGAGAGCTGCAGGGTGGGTGGGCC
  CCTAGCAGTGTTGGGCCCTTCTCGATCTTCTGAGGACCTGGCTGGCCCCCTTCCTTCCTCAGTCCCTTCC
  TCTACAACATCCTCAAAGCCAAAGCTCAAGAAGCGCTTCTCCCTCCGCTCAGTGGGCCGTTCAGTCAGAG
  GCTCTGTTCGAGGCATCCTGCAGTGGCGGGGTGCCGTTGACTCGCCCTCCCAAGCTGGGCCTCTGGAGAC
  CACATCCGGCCCTCCAGTTCTAGGTGGAAACAGCAACTCCAACTCCTCGGGTGGTGCTGGGACAGTTGGT
  AGGGCATTGGCTAATGATGGCACATCCCCTGGGGAGAGATGGACTCATCGATTTGAGAGGCTGAGGCTAA
  GTCGTGGAGGGGGAACCCTGAAAGACGGAGCAGGAATGATACAGAGAGAAGAGCTGCTGAGTTTCATGGG
  GGCTGAAGAGGCTGCCCCTGACCCAGCAGGAGTGGGTCGTGGAGGAGGGGCAGCTGGGCTGACCTCAGGA
  GGAGGAGGGCAGCCTCAGTGGCAAAAGTGTCGCTTACTGCTCCGGAGTGAAGGAGAAGGAGGAGGAGGAA
  GTCGCTTGGAGTTCTTTGTACCACCCAAGGCGTCCCGACCCCGTCTCAGCATTCCCTGCTCTACTATTAC
  TGATGTCCGCACAGCCACAGCCCTAGAGATGCCTGACAGGGAGAACACGTTTGTGGTTAAGGTAGAAGGC
  CCTTCAGAGTACATCCTGGAGACAAGTGATGCGCTTCATGTGAAGGCCTGGGTGTCTGACATCCAGGAAT
  GCCTAAGCCCCGGACCCTGTCCTGCTATCAGCCCCCGTCCCATGACCCTTCCCCTGGCCCCTGGGACCTC
  CTTCTTCACAAAGGATAACACAGACAGCCTGGAGTTGCCCTGCCTGAATCATTCAGAGAGTCTGCCTAGC
  CAGGATCTGCTGCTGGGACCCAGCGAGAGTAACGACCGCCTGTCGCAGGGAGCTTATGGGGGCCTCTCAG
  ACCGGCCATCGGCGTCCTTCTCCCCTAGTTCTGCCTCCATTGCTGCCTCCCATTTTGATTCGATGGAACT
  GCTTCCTCCAGAATTGCCTCCTCGAATTCCCATTGAGGAGGGGCCTCCAGCAGGGACAGTTCATCCCCTC
  TCTACCCCCTACCCTCCCCTGGATACTCCAGAAGCAGCCACAGGATCGTTCCTGTTCCAAGGGGAGTCAG
  AGGGGGGTGAGGGGGATCAGCCCCTCTCAGGCTATCCTTGGTTCCACGGCATGCTCTCTCGACTCAAAGC
  TGCCCAGTTAGTGTTAGAAGGAGGCACTGGCTCCCATGGTGTCTTCTTGGTACGCCAGAGTGAGACAAGG
  CGTGGTGAATATGTCCTCACTTTCAACTTCCAGGGCAAGGCCAAGCACCTGCGCTTGTCACTAAATGAGG
  AGGGACAGTGCCGGGTCCAACATCTGTGGTTCCAGTCCATTTTCGATATGCTTGAGCACTTCCGGGTGCA
  CCCCATCCCTCTGGAGTCTGGAGGCTCCAGTGATGTTGTCCTTGTCAGCTATGTGCCCTCCCAGCGGCAG
  CAGGGTGAGCAGAGCAGGTCTGCAGGGGAGGAGGTGCCCGTGCACCCAAGAAGTGAGAACGGAGCACCTC
  CCGTGACCCAGCCCAGCCCTCTGAACCCCCTCCATGGACAGATCCCCCACATCCTGGGGCAGAAGAGGCG
  TCGGGGGCGCCAGAAGTTGCGGCAGCCACAGCCGCAGCAGCCAAAGAGAGGCAAGAGAAAGAGAAAGCGG
  GCAGTGGAGGGGTCCAGGAAGAGCTGGTCCCCGTGGCTGAGCTGGTCCCCATGGTTGAATTGGAAGAGGC
  CA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230985 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNGAPSPEDGVFPSPPALPPPPPPSWQEFCESHARAAALDLARRFRLYLASHPQYAEPGAEAAFSGRFAE
  LFLQHFEAEVARASGSLSPPVLAPLSPGVEIPPSHDLSLESCRVGGPLAVLGPSRSSEDLAGPLPSSVPS
  STTSSKPKLKKRFSLRSVGRSVRGSVRGILQWRGAVDSPSQAGPLETTSGPPVLGGNSNSNSSGGAGTVG
  RALANDGTSPGERWTHRFERLRLSRGGGTLKDGAGMIQREELLSFMGAEEAAPDPAGVGRGGGAAGLTSG
  GGGQPQWQKCRLLLRSEGEGGGGSRLEFFVPPKASRPRLSIPCSTITDVRTATALEMPDRENTFVVKVEG
  PSEYILETSDALHVKAWVSDIQECLSPGPCPAISPRPMTLPLAPGTSFFTKDNTDSLELPCLNHSESLPS
  QDLLLGPSESNDRLSQGAYGGLSDRPSASFSPSSASIAASHFDSMELLPPELPPRIPIEEGPPAGTVHPL
  STPYPPLDTPEAATGSFLFQGESEGGEGDQPLSGYPWFHGMLSRLKAAQLVLEGGTGSHGVFLVRQSETR
  RGEYVLTFNFQGKAKHLRLSLNEEGQCRVQHLWFQSIFDMLEHFRVHPIPLESGGSSDVVLVSYVPSQRQ
  QGEQSRSAGEEVPVHPRSENGAPPVTQPSPLNPLHGQIPHILGQKRRRGRQKLRQPQPQQPKRGKRKRKR
  AVEGSRKSWSPWLSWSPWLNWKRP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001289541
• ORF Size: 2175 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289541.1, NP_001276470.1
• RefSeq Size: 2959 bp
• RefSeq ORF: 2175 bp
• Locus ID: 20399
• UniProt ID: Q91ZM2
• Cytogenetics: 7 69.06 cM
• MW: 77.6 kDa
• Gene Summary: Adapter protein for several members of the tyrosine kinase receptor family. Involved in multiple signaling pathways mediated by Janus kinase (JAK) and receptor tyrosine kinases, including the receptors of insulin (INS), insulin-like growth factor I (IGF1), nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF), platelet-derived growth factor (PDGF) and fibroblast growth factors (FGFs). In growth hormone (GH) signaling, autophosphorylated ('Tyr-813') JAK2 recruits SH2B1, which in turn is phosphorylated by JAK2 on tyrosine residues. These phosphotyrosines form potential binding sites for other signaling proteins. GH also promotes serine/threonine phosphorylation of SH2B1 and these phosphorylated residues may serve to recruit other proteins to the GHR-JAK2-SH2B1 complexes, such as RAC1. In leptin (LEP) signaling, binds to and potentiates the activation of JAK2 by globally enhancing downstream pathways. In response to leptin, binds simultaneously to both, JAK2 and IRS1 or IRS2, thus mediating formation of a complex of JAK2, SH2B1 and IRS1 or IRS2. Mediates tyrosine phosphorylation of IRS1 and IRS2, resulting in activation of the PI 3-kinase pathway. Acts as positive regulator of NGF-mediated activation of the Akt/Forkhead pathway; prolongs NGF-induced phosphorylation of AKT1 on 'Ser-473' and AKT1 enzymatic activity. Enhances the kinase activity of the cytokine receptor-associated tyrosine kinase JAK2 and of other receptor tyrosine kinases, such as FGFR3 and NTRK1. For JAK2, the mechanism seems to involve dimerization of both, SH2B1 and JAK2. Enhances RET phosphorylation and kinase activity (By similarity). Isoforms seem to be differentially involved in IGF-I and PDGF-induced mitogenesis, according the order: isoform 3 > isoform 4 > isoform 1 > isoform 2.[UniProtKB/Swiss-Prot Function]