Usp44 (NM_001206851) Mouse Tagged ORF Clone

CAT#: MR230956

Usp44 (myc-DDK-tagged) - Mouse ubiquitin specific peptidase 44 (Usp44), transcript variant 1

Product Images

Circular map for MR230956

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Usp44
• Synonyms: E430004F17Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230956 representing NM_001206851
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATAGGTGCAAGCACGTTGAGCAGTTGCAGCTTGCTCAAGGCCATTCCATCTTGGACCCTCAGAAAT
  GGTACTGTATGGTCTGCAACACAACCGAGTCCATTTGGGCTTGCCTCAGCTGTTCCCACGTCGCCTGTGG
  GAAGTACATCCAAGAGCACGCACTGAAGCACTTTCAAGAAAGCAGCCATCCCGTTGCCTTCGAGGTGAAC
  GACATGTACGCTTTTTGTTATCTTTGTAACGATTATGTTCTAAACGATAACGCAGCCGGAGACCTGAAGT
  CACTACGGAGTACACTAAGTACAATCAAAAGTAAAAAGTACCCCTGTGTGGTTCCGAGTGACTCGGTTTT
  ACATCCTGTGGATGCACAGGACCGTGTTTACTCCTTACTCGACGGCACCCAATCTCTGCCCGGAAACGAG
  GATCCAACGTGCGCCGCCCTCTGGCACAGGAGACGGGTGCTCATGGGGAAAGCCTTTCGAACTTGGTTCG
  AACAGTCAGCAATTGGAAGGAAAGGGCAAGAGCCAACTCAGGAGAGAATGGTAGCAAAACGAGAGGCGAA
  GAGAAGGCAGCAGCAGGAGTTGGAGCAGCAGATGAAAGCCGAGCTGGAAAGCACACCTCCGCGGAAGAGT
  TTACGCTTACAAGGCTCCTCGGAGGAGGCGGCCACAATCGAAATCGTGCCAGTGCGGGCACCGCCCCCAC
  CTCCCGCATCCCCAGCAAAGGACAAAGCCGCGCTACCTACTTCGGAAGACAGGACTTTTAAAAAAGTAAG
  TGACTCCTTAATCAAACGAAGGCCCATGGTAACTCCTGGTGTAACCGGACTGAGAAACTTAGGAAATACT
  TGCTATATGAATTCTGTTCTTCAAGTGTTGAGTCATTTACTCATTTTTCGACAATGTTTTTTAAAGCTTG
  ACCTGAACCAGTGGCTGGCTGTGGCTGCCAGCGATAAGGCCCGATCCTATAAGCACTCAGCCGTCACGGA
  GGCGGCGGCACAGCAGATGAACGAAGGGCAAGAGAAAGAGAAAGGCTTCGTATGCTCCAGACATTCGGGT
  TTATCCTCAGGCCTGAGCGGAGGGGCCTCAAAAGGTCGGAACATGGAGCTTATTCAGCCAAGGGAGCCCA
  GTTCCCCGTACAGTTCTCTCTGCCATGAATTGCATATCCTGTTCCAAGTCATGTGGTCTGGAGAGTGGGC
  CTTGGTCTCACCGTTTGCCATGCTTCACTCCGTGTGGAGGCTGATCCCTGCTTTCCGCGGTTACGCCCAG
  CAGGATGCTCAGGAATTTCTTTGTGAACTTCTGGATAAGATACAACGTGAACTAGAGACAACCGGAACCA
  AGTTACCAGCCCTCATCCCCACTTCCCAAAGGAGACTTATTGAGCAAGTTCTGAATGTGGTGAATAACAT
  TTTTCACGGACAATTTCTTAGTCAGGTTACATGTCTTGCTTGTGACAACAAATCAGATACCATAGAATCC
  TTCTGGGACTTGTCCCTGGAGTTTCCAGAGAGATACCAGTGCAGTGGGAAAGATGCTGCCTCCCAGCCGT
  GCCTGGTTACTGACATGTTGGACAAATTTACAGAAACAGAAGCTTTAGAAGGGAAAATCTACATGTGTGA
  CCATTGTAACTCAAAGCGTAGAAAGTTTTCGTCAAAATCAGTTGTATTCACAGAAGCCCAGAAGCAGCTT
  ATGATATGCCATCTACCTCAGGTTCTCAGACTGCACCTCAAACGGTTCAGATGGTCAGGACGTAATAACC
  GAGAGAAGATTGGTGTTCATGTTGTCTTTGAGGAAACCTTAAACATGGAGCCTTATTGCTGCAGGGAGAC
  CCTGAACGCTCTCAGACCAGAGTGCTTTCTCTATAATTTATCTGCTGTCGTAATTCACCATGGAAAAGGA
  TTTGGCTCAGGACACTACACTGCCTACTGCTACAATTCCGAAGGAGGGTTCTGGGTCCACTGCAACGATT
  CCAAGCTGAGCATGTGCACGATGGAAGAAGTACGCAAGGCCCAAGCTTATATCTTATTTTATACTCAGCG
  AGTTACTGAGAATGGACATTCGAAACTCTTGCCTCCCGAGCTCCTGTCCAATAGCCAGCACCCCAGTAAG
  GAGACTGATGCCTCTTCTAATGAAGTCCTTAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230956 representing NM_001206851
  Red=Cloning site Green=Tags(s)
  
  MDRCKHVEQLQLAQGHSILDPQKWYCMVCNTTESIWACLSCSHVACGKYIQEHALKHFQESSHPVAFEVN
  DMYAFCYLCNDYVLNDNAAGDLKSLRSTLSTIKSKKYPCVVPSDSVLHPVDAQDRVYSLLDGTQSLPGNE
  DPTCAALWHRRRVLMGKAFRTWFEQSAIGRKGQEPTQERMVAKREAKRRQQQELEQQMKAELESTPPRKS
  LRLQGSSEEAATIEIVPVRAPPPPPASPAKDKAALPTSEDRTFKKVSDSLIKRRPMVTPGVTGLRNLGNT
  CYMNSVLQVLSHLLIFRQCFLKLDLNQWLAVAASDKARSYKHSAVTEAAAQQMNEGQEKEKGFVCSRHSG
  LSSGLSGGASKGRNMELIQPREPSSPYSSLCHELHILFQVMWSGEWALVSPFAMLHSVWRLIPAFRGYAQ
  QDAQEFLCELLDKIQRELETTGTKLPALIPTSQRRLIEQVLNVVNNIFHGQFLSQVTCLACDNKSDTIES
  FWDLSLEFPERYQCSGKDAASQPCLVTDMLDKFTETEALEGKIYMCDHCNSKRRKFSSKSVVFTEAQKQL
  MICHLPQVLRLHLKRFRWSGRNNREKIGVHVVFEETLNMEPYCCRETLNALRPECFLYNLSAVVIHHGKG
  FGSGHYTAYCYNSEGGFWVHCNDSKLSMCTMEEVRKAQAYILFYTQRVTENGHSKLLPPELLSNSQHPSK
  ETDASSNEVLS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001206851
• ORF Size: 2133 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001206851.3
• RefSeq Size: 2533 bp
• RefSeq ORF: 2136 bp
• Locus ID: 327799
• UniProt ID: Q8C2S0
• Cytogenetics: 10 C2
• MW: 80.9 kDa
• Gene Summary: Deubiquitinase that plays a key regulatory role in the spindle assembly checkpoint or mitotic checkpoint by preventing premature anaphase onset. Acts by specifically mediating deubiquitination of CDC20, a negative regulator of the anaphase promoting complex/cyclosome (APC/C). Deubiquitination of CDC20 leads to stabilize the MAD2L1-CDC20-APC/C ternary complex (also named mitotic checkpoint complex), thereby preventing premature activation of the APC/C. Promotes association of MAD2L1 with CDC20 and reinforces the spindle assembly checkpoint. Acts as a negative regulator of histone H2B (H2BK120ub1) ubiquitination.[UniProtKB/Swiss-Prot Function]