Arhgap24 (NM_001286468) Mouse Tagged ORF Clone

CAT#: MR230797

Arhgap24 (myc-DDK-tagged) - Mouse Rho GTPase activating protein 24 (Arhgap24), transcript variant 3

Product Images

Circular map for MR230797

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Arhgap24
• Synonyms: 0610025G21Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230797 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAGCCAATCATGAGAGCTATCTCCTCATGGCGAGCACCCAGAATGACATGGAAGACTGGGTGAAGT
  CCATCCGCCGAGTCATATGGGGACCTTTTGGAGGAGGTATTTTTGGACAGAAACTGGAAGACACAGTCCG
  ATATGAAAAGAGGTACGGGAACCGGCTGGCACCCATGCTGGTGGAGCAGTGTGTGGACTTCATCCGACAG
  CGGGGGCTGAAGGAAGAAGGCCTCTTCCGGCTGCCGGGCCAGGCGAACCTGGTCAAGGAGCTCCAGGATG
  CGTTCGACTGTGGAGAGAAGCCCTCCTTCGACAGCAACACCGACGTCCACACTGTGGCATCCCTGCTGAA
  ATTGTATCTCCGAGAACTTCCAGAGCCCGTCGTTCCTTATGCAAAGTATGAAGACTTTTTGTCCTGCGCC
  ACGCTGCTCAGTAAGGAGGAAGAAGCAGGTGTTAAGGAGCTAATGAAACAAGTGAAGAGTTTGCCTGTGG
  TTAACTACAACCTTCTGAAGTACATTTGCAGGTTCCTGGATGAAGTGCAGTCCTATTCAGGAGTTAACAA
  GATGAGCGCACAGAACTTGGCAACTGTCTTCGGTCCTAACATCCTGCGCCCAAAGGTGGAAGATCCCTTG
  ACCATCATGGAGGGCACTGTGGTGGTCCAGCAGTTAATGTCAGTGATGATTAGTAAGCATGATCGACTCT
  TCCCCAAAGACACAGAACCACAAAGCAAGCCCCAAGACGGCCCGAACAGCAATAATAACGATGGTCATAA
  GAAAGCCACCATGGGTCAGTTGCAGAACAAAGAAAACAATAATACCAAGGAGAGCCCCGTCAGAAGGTGC
  TCTTGGGACAAGCCTGAATCCCCCCAGAGAAGCAGCGTGGACAATGGATCCCCCACAGCCCTATCAGGCA
  GCAAAACCAACAGCCCAAGGAACAGTATCCACAAGCTGGATATTTCCAGGAGCCCCCCTCTCATGGTCAA
  AAAGAACCCAGCCTTCAACAAGGGCAGTGGGATTGTCACCAACGGGTCATTCAGCAGCAGCAATGCAGAA
  GGTGTCGAGAAGCCTCAAACTACTCCTAATGGGAGTTTACAAGCCAGAAGGACCTCTTCACTGAAGAGTT
  CTGGTACCAAAATGGGCACTCACAGTGTACAGAATGGCACTGTCCGCATGGGTATTCTGAACACTGACAC
  ACTAGGGAATTCCTTGAACGGCCGGAGCATGAGTTGGCTGCCCAATGGCTATGTGACCCTGAGAGATAAC
  AAACAGAAGGAGCCAGCAGGAGAGTCAGGCCAGCACAACAGACTCTCCACCTACGACAATGTCCACCAGC
  AGTTCTCCTCGATGAGCCTCGACGACAAGCACAGCGTGGACAGTGCCACGTGGTCCACCTCCTCCTGCGA
  AATCTCCCTCCCTGAGAACTCCAATTCCTGCCGCTCCTCTACCACCACGTGCCCAGAGCAAGACTTCTAC
  GTTGGGAACTTTGAGGACCCTGTTTTAGACGGCCCACCTCAGGATGATCTTTCCCATCCCGGGGACTATG
  AGAACAAAAGTGACCGCAGGAGCGTAGGGGGCCGAAGCAGCCGCGCCACGAGCAGCAGCGATAACAGCGA
  GACATTCGTGGGCAACACCAGCAGCAACCACAGTGCACTTCACAGTTTAGTTTCCAGCCTGAAGCAGGAG
  ATGACCAAACAGAAGATCGAGTATGAGTCCAGGATAAAAAGCTTAGAACAGCGGAATTTGACTTTGGAAA
  CGGAAATGCTGAGCCTCCATGACGAACTGGATCAAGAGAGGAAAAAGTTCACCATGATAGAAATCAAGAT
  GAGAAACGCTGAGCGCGCAAAGGAGGATGCGGAGAAAAGGAACGACATGCTCCAGAAGGAGATGGAGCAG
  TTCTTCTCCACATTTGGAGACCTGACAGTGGAACCCAGGAGAAGCGAGAGAGGAAACACAATCTGGATCC
  AG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230797 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTANHESYLLMASTQNDMEDWVKSIRRVIWGPFGGGIFGQKLEDTVRYEKRYGNRLAPMLVEQCVDFIRQ
  RGLKEEGLFRLPGQANLVKELQDAFDCGEKPSFDSNTDVHTVASLLKLYLRELPEPVVPYAKYEDFLSCA
  TLLSKEEEAGVKELMKQVKSLPVVNYNLLKYICRFLDEVQSYSGVNKMSAQNLATVFGPNILRPKVEDPL
  TIMEGTVVVQQLMSVMISKHDRLFPKDTEPQSKPQDGPNSNNNDGHKKATMGQLQNKENNNTKESPVRRC
  SWDKPESPQRSSVDNGSPTALSGSKTNSPRNSIHKLDISRSPPLMVKKNPAFNKGSGIVTNGSFSSSNAE
  GVEKPQTTPNGSLQARRTSSLKSSGTKMGTHSVQNGTVRMGILNTDTLGNSLNGRSMSWLPNGYVTLRDN
  KQKEPAGESGQHNRLSTYDNVHQQFSSMSLDDKHSVDSATWSTSSCEISLPENSNSCRSSTTTCPEQDFY
  VGNFEDPVLDGPPQDDLSHPGDYENKSDRRSVGGRSSRATSSSDNSETFVGNTSSNHSALHSLVSSLKQE
  MTKQKIEYESRIKSLEQRNLTLETEMLSLHDELDQERKKFTMIEIKMRNAERAKEDAEKRNDMLQKEMEQ
  FFSTFGDLTVEPRRSERGNTIWIQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286468
• ORF Size: 1965 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286468.1, NP_001273397.1
• RefSeq Size: 2751 bp
• RefSeq ORF: 1965 bp
• Locus ID: 231532
• UniProt ID: Q8C4V1
• Cytogenetics: 5 E5
• MW: 73.3 kDa
• Gene Summary: Rho GTPase-activating protein involved in cell polarity, cell morphology and cytoskeletal organization. Acts as a GTPase activator for the Rac-type GTPase by converting it to an inactive GDP-bound state. Controls actin remodeling by inactivating Rac downstream of Rho leading to suppress leading edge protrusion and promotes cell retraction to achieve cellular polarity. Able to suppress RAC1 and CDC42 activity in vitro. Overexpression induces cell rounding with partial or complete disruption of actin stress fibers and formation of membrane ruffles, lamellipodia, and filopodia. Isoform 2 is a vascular cell-specific GAP involved in modulation of angiogenesis (By similarity).[UniProtKB/Swiss-Prot Function]