Dnm1l (NM_001276341) Mouse Tagged ORF Clone

CAT#: MR230677

Dnm1l (myc-DDK-tagged) - Mouse dynamin 1-like (Dnm1l), transcript variant 4

Product Images

Circular map for MR230677

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dnm1l
• Synonyms: 6330417M19Rik; AI450666; Dlp1; Dnm; Dnmlp1; Dr; Drp1; pyt; python
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230677 representing NM_001276341
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTTACACAGATTTTGATGAAATTCGACAAGAAATTGAAAATGAAACTGAAAGAATTTCAGGAAATA
  ATAAGGGGGTAAGCCCTGAGCCAATCCATCTCAAGGTTTTCTCGCCCAACGTTGTCAACCTGACACTTGT
  GGATTTACCGGGAATGACCAAAGTACCTGTAGGCGATCAGCCCAAGGACATCGAGCTTCAGATCAGAGAA
  CTTATTCTTCGGTTCATCAGTAATCCCAATTCCATTATCCTCGCCGTCACTGCTGCAAATACAGATATGG
  CAACATCAGAAGCACTCAAGATTTCCAGAGAGGTAGATCCAGATGGGCGCAGAACTCTAGCTGTAATCAC
  TAAACTTGATCTCATGGATGCGGGTACTGATGCCATGGATGTATTGATGGGAAGGGTTATTCCAGTCAAG
  CTTGGAATAATTGGAGTAGTTAACAGAAGCCAACTGGATATTAACAATAAGAAGAGTGTAACTGATTCAA
  TCCGTGATGAGTATGCTTTTCTTCAAAAGAAGTACCCATCTCTGGCCAACAGAAATGGAACAAAGTATCT
  TGCTAGGACCCTGAATAGGTTACTTATGCATCATATCAGAGATTGTTTACCAGAGCTGAAAACAAGAATA
  AATGTCTTAGCTGCTCAGTATCAGTCTCTTCTAAATAGCTATGGTGAACCGGTGGATGATAAAAGTGCTA
  CTTTACTCCAGCTTATTACCAAATTTGCCACAGAGTATTGTAACACGATTGAAGGAACCGCAAAGTACAT
  TGAAACTTCTGAGCTATGCGGTGGTGCTAGGATTTGTTATATTTTCCATGAGACTTTCGGGCGAACCTTA
  GAATCTGTGGACCCACTAGGTGGCCTTAACACTATTGACATCCTGACTGCCATCAGAAATGCTACTGGCC
  CCCGTCCTGCTTTATTTGTGCCTGAAGTTTCATTTGAGTTACTGGTCAAACGTCAGATTAAGCGTCTAGA
  AGAGCCCAGCCTACGGTGTGTGGAGCTGGTCCATGAGGAGATGCAGAGGATCATTCAGCATTGTAGCAAT
  TACAGCACACAGGAATTGTTACGGTTCCCTAAACTTCACGATGCCATAGTTGAAGTAGTGACCTGTCTTC
  TTCGTAAAAGGTTGCCCGTGACAAATGAAATGGTGCATAACTTAGTGGCAATTGAGCTAGCGTATATCAA
  CACAAAACACCCCGACTTTGCTGATGCCTGTGGGCTAATGAACAATAATATAGAGGAACAAAGAAGAAAC
  AGGCTAGCAAGAGAGCTGCCTTCAGCTGGATCACGGGACAAGTTAATTCAGGACAACAGAAGAGAAACTA
  AAAATGTCCCATCTGCAGGTGGTGGGATTGGAGACGGTGGTCAGGAACCAACAACAGGCAACTGGAGAGG
  AATGCTGAAAACTTCAAAAGCTGAAGAATTACTTGCTGAAGAAAAATCAAAACCAATTCCAATTATGCCA
  GCAAGTCCACAGAAAGGCCATGCTGTCAATTTGCTAGATGTGCCAGTTCCAGTTGCAAGAAAACTGTCTG
  CCCGAGAACAGCGAGATTGTGAGGTTATTGAAAGACTTATCAAATCATATTTTCTAATTGTCAGAAAGAA
  TATTCAAGACAGTGTCCCAAAGGCAGTAATGCATTTTTTGGTTAATCATGTGAAAGATACTCTTCAGAGT
  GAACTGGTAGGGCAGCTGTATAAGTCATCCTTATTAGATGACCTTCTGACTGAATCCGAGGACATGGCCC
  AACGAAGAAAAGAAGCAGCGGATATGCTGAAGGCATTACAAGGAGCCAGTCAAATTATTGCTGAAATCCG
  AGAGACTCATCTTTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230677 representing NM_001276341
  Red=Cloning site Green=Tags(s)
  
  MLYTDFDEIRQEIENETERISGNNKGVSPEPIHLKVFSPNVVNLTLVDLPGMTKVPVGDQPKDIELQIRE
  LILRFISNPNSIILAVTAANTDMATSEALKISREVDPDGRRTLAVITKLDLMDAGTDAMDVLMGRVIPVK
  LGIIGVVNRSQLDINNKKSVTDSIRDEYAFLQKKYPSLANRNGTKYLARTLNRLLMHHIRDCLPELKTRI
  NVLAAQYQSLLNSYGEPVDDKSATLLQLITKFATEYCNTIEGTAKYIETSELCGGARICYIFHETFGRTL
  ESVDPLGGLNTIDILTAIRNATGPRPALFVPEVSFELLVKRQIKRLEEPSLRCVELVHEEMQRIIQHCSN
  YSTQELLRFPKLHDAIVEVVTCLLRKRLPVTNEMVHNLVAIELAYINTKHPDFADACGLMNNNIEEQRRN
  RLARELPSAGSRDKLIQDNRRETKNVPSAGGGIGDGGQEPTTGNWRGMLKTSKAEELLAEEKSKPIPIMP
  ASPQKGHAVNLLDVPVPVARKLSAREQRDCEVIERLIKSYFLIVRKNIQDSVPKAVMHFLVNHVKDTLQS
  ELVGQLYKSSLLDDLLTESEDMAQRRKEAADMLKALQGASQIIAEIRETHLW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001276341
• ORF Size: 1836 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001276341.1, NP_001263270.1
• RefSeq Size: 3829 bp
• RefSeq ORF: 1839 bp
• Locus ID: 74006
• UniProt ID: Q8K1M6
• Cytogenetics: 16 A2
• MW: 69.2 kDa
• Gene Summary: This gene encodes a member of the dynamin family. The encoded protein is localized to the cytoplasm and mitochondrial membrane, is involved in mitochondrial and peroxisomal division, and is essential for mitochondrial fission. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 2. [provided by RefSeq, Feb 2013]