Inpp5e (NM_001290437) Mouse Tagged ORF Clone

CAT#: MR230658

Inpp5e (myc-DDK-tagged) - Mouse inositol polyphosphate-5-phosphatase E (Inpp5e), transcript variant 2

Product Images

Circular map for MR230658

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Inpp5e
• Synonyms: 72kDa; 1200002L24Rik; mKIAA0123
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230658 representing NM_001290437
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCATCCAAGTCAGCTTGCCTGCGTCACACTGAGGCACCTGGGCAGCTAGAAGGCAGAATGCTCCAGG
  GACAGCCTCCGAACACGGAAAAGAAGCTCATCCCAACACCAGGCTTCTTACCAGCCTCTGACTCTCAGGG
  TTCAGAGACAAACCCCATGCCTCCTTTCAGCATTCCAGCAAAAACAAGCAACCAGAACCCACAAACTAAG
  GCAAATCTCATCACCCCACAGCCACCCATTAGACCCAAGTTGGAGCGAACCCTGTCCCTCGATGACAAGG
  GCTGGAGAAGGAGGCGTTTCCGGGGTAGTCAGGAGGATCTGACTGTCCAGAATGGGGCCAGTCCCTGCAG
  GGGCTCCTTGCAAGACTCAGTAGCCCAGTCCCCTGCCTACAGCCGTCCCCTGCCCTGCCTCAGCACATCC
  TTGCAGGAGATACCTAAGTCCCGAAGGGCCACAGGCAGTGAGGGAGGGAGCCCATCCTTGTGGAGTGACT
  GTCTTTCTGGAATGATCAGCACCTCCTTGGACCTCCTGCACAGAGATGCTGCCTCAGGTGGGCCCCCCTC
  CAGGTTGGCAAGTTTGCATGCCTCACACACACCACCAGCTATGGACCTCAGCATAGCCTCCAGCTCCCTG
  AGAACAGCAAACAAGGTTGACCCCGAGCACACGGACTACAAGCTCCGCATGCAGACCAGACTGGTCAGGG
  CCCACAGCAACCTGGGCCCTAGCAGACCCCGGAGCCCCTTGGCTGGTGACGATCACTCCATTCACTCAGC
  CAGATCTTTCAGCCTTCTGGCCCCCATCCGCACCAAGGACATCAGAAGCAGGAGCTATCTGGAGGGAAGT
  CTTCTGGCCAGTGGGGCCCTGCTAGGAGCAGAAGAGCTGGCCAGGTACTTCCCAGACCGAAACATGGCTC
  TCTTCGTGGCTACCTGGAACATGCAGGGCCAGAAGGAGCTCCCAGCGAGCCTGGATGAGTTTCTGCTCCC
  CACCGAGGCTGACTACACTCAGGACCTGTATGTCATTGGAATTCAGGAGGGCTGCTCTGACAGGCGGGAG
  TGGGAGACACGCCTGCAGGAGACACTGGGCCCTCAGTATGTACTGCTGTCATCAGCAGCACATGGGGTCC
  TGTACATGTCCCTGTTTATCCGTAGGGACCTCATCTGGTTCTGCTCAGAGGTCGAGTACTCCACAGTAAC
  TACACGCATCGTGTCTCAGATCAAGACCAAGGGGGCCCTGGGCGTCAGCTTCACCTTTTTTGGCACCTCC
  TTCCTCTTCATCACATCTCACTTCACCTCTGGAGATGGGAAGGTAGCAGAGCGGCTACTGGACTACAGCA
  GAACCATCCAAGCCCTAGCCCTGCCCCGGAATGTGCCAGACACAAATCCCTACCGCTCTAGTGCAGGGGA
  TGTCACTACCCGGTTTGATGAGGTCTTCTGGTTTGGGGACTTCAACTTCCGCCTGAGTGGTGGACGAGTG
  GCTGTGGAGGCCTTCCTGAAGCAGAAACCTGAGGTGGATGTGCTGGCTCTCCTCCAACACGACCAGCTCA
  CCCGGGAGATGAAGAAAGGGTCCATCTTCAGGGGCTTTGAGGAGGCAGAGATTCACTTTCTTCCATCCTA
  CAAGTTTGACATTGGGAAGGACACCTACGACAGCACCTCCAAGCAAAGGACACCCTCCTACACAGACCGA
  GTCCTATACAAAAGCCGTCACAAGGGTGACATCTGTCCCATGAAGTATTCCTCTTGTCCTGGGATCAAGA
  CTTCAGACCACCGTCCTGTGTATGGCTTGTTCCAGGTGAAAGTGAGGCCAGGACGAGACAAGAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230658 representing NM_001290437
  Red=Cloning site Green=Tags(s)
  
  MPSKSACLRHTEAPGQLEGRMLQGQPPNTEKKLIPTPGFLPASDSQGSETNPMPPFSIPAKTSNQNPQTK
  ANLITPQPPIRPKLERTLSLDDKGWRRRRFRGSQEDLTVQNGASPCRGSLQDSVAQSPAYSRPLPCLSTS
  LQEIPKSRRATGSEGGSPSLWSDCLSGMISTSLDLLHRDAASGGPPSRLASLHASHTPPAMDLSIASSSL
  RTANKVDPEHTDYKLRMQTRLVRAHSNLGPSRPRSPLAGDDHSIHSARSFSLLAPIRTKDIRSRSYLEGS
  LLASGALLGAEELARYFPDRNMALFVATWNMQGQKELPASLDEFLLPTEADYTQDLYVIGIQEGCSDRRE
  WETRLQETLGPQYVLLSSAAHGVLYMSLFIRRDLIWFCSEVEYSTVTTRIVSQIKTKGALGVSFTFFGTS
  FLFITSHFTSGDGKVAERLLDYSRTIQALALPRNVPDTNPYRSSAGDVTTRFDEVFWFGDFNFRLSGGRV
  AVEAFLKQKPEVDVLALLQHDQLTREMKKGSIFRGFEEAEIHFLPSYKFDIGKDTYDSTSKQRTPSYTDR
  VLYKSRHKGDICPMKYSSCPGIKTSDHRPVYGLFQVKVRPGRDKN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001290437
• ORF Size: 1815 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290437.1, NP_001277366.1
• RefSeq Size: 3855 bp
• RefSeq ORF: 1818 bp
• Locus ID: 64436
• UniProt ID: Q9JII1
• Cytogenetics: 2 A3
• MW: 67.7 kDa
• Gene Summary: Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3), phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) and phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Specific for lipid substrates, inactive towards water soluble inositol phosphates. Specific for lipid substrates, inactive towards water soluble inositol phosphates (By similarity) (PubMed:10806194). Plays an essential role in the primary cilium by controlling ciliary growth and phosphoinositide 3-kinase (PI3K) signaling and stability (PubMed:19668215).[UniProtKB/Swiss-Prot Function]