Tnk2 (NM_001289443) Mouse Tagged ORF Clone

CAT#: MR230345

  • TrueORF®

Tnk2 (myc-DDK-tagged) - Mouse tyrosine kinase, non-receptor, 2 (Tnk2), transcript variant 3


  "NM_001289443" in other vectors (1)


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Reconstitution Protocol

USD 530.00

4 Weeks*

Size
    • 10 ug

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Frequently bought together (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
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DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
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USD 160.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 59.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

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Other products for "Tnk2"

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Tnk2
Synonyms Ack; Ack-1; Ack1; Cdgip; Pyk1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR230345 representing NM_001289443
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACCCTCCTGACCTGCTGAGTGTGGAACTGAGCACCTCCCGACCCACCCAGCACCTAGGACGGGTGA
AAAAGCCAACATACGACCCTGTGAGTGAGGACCCAGACCCCCTGTCCAGCGACTTCAAGAGGCTTGGCCT
GAGGAAGCCAGCCCTGCCTCGAGGGCTGTGGCTGGCAAAGCCCTCAGCCCGAGTGCCAGGCACCAAGGCA
GACCGCAGCAGTGGGGGTGAGGTCACACTCATCGACTTCGGTGAGGAGCCTGTGGTCCCAACCCCTCGGC
CCTGTGCACCCTCCTTGGCACAGTTGGCCATGGATGCCTGCTCCTTGCTGGACAAGACACCACCACAGAG
CCCCACACGGGCACTGCCACGACCTTTACACCCCACCCCTGTGGTAGACTGGGACGCGCGTCCGCTGCCC
CCGCCCCCTGCCTATGACGACGTGGCCCAGGATGAGGATGACTTTGAGGTCTGCTCCATCAACAGCACGC
TAGTAGGTGCAGGCCTCCCTGCTGGGCCTAGCCAAGGCGAGACCAATTACGCCTTTGTACCTGAGCAGGC
GCAGATGCCCCCTGCCCTGGAGGATAACCTGTTCCTTCCACCCCAGGGCGGAGGCAAGCCGCCCAGCTCA
GTGCAGACTGCAGAGATTTTCCAGGCACTGCAGCAGGAGTGTATGCGGCAGCTACAGGTCCCCACTGGCC
AGCTGACCCCCTCCCCGACCCCAGGAGGTGATGACAAGCCCCAGGTGCCACCCCGGGTACCTATTCCCCC
TCGGCCCACGCGTCCACGTGTGGAGCTATCTCCAGCTCCCTCGGGTGAGGAAGAGACAAGCCGGTGGCCT
GGACCTGCCTCGCCCCCCCGAGTGCCTCCCCGGGAACCTCTGTCTCCTCAAGGTTCAAGGACCCCAAGCC
CCCTAGTGCCACCTGGCAGCTCTCCACTACCGCATCGGCTCTCTAGCTCACCTGGAAAGACCATGCCCAC
CACTCAAAGCTTTGCCTCAGACCCTAAGTATGCCACTCCACAAGTGATCCAGGCTCCTGGCCCACGGGCA
GGCCCCTGCATCCTGCCCATTGTCCGCGATGGCAGGAAGGTCAGCAGCACTCATTACTACCTGCTGCCTG
AGCGCCCTCCTTACCTGGAACGCTATCAGCGCTTCCTGCGGGAGGCCCAGAGCCCGGAAGAGCCGGCCGC
CCTGCCTGTGCCCCCGCTGTTGCCCCCGCCCAGTACTCCAGCCCCTGCTGCCCCCACTGCCACCGTCAGA
CCTATGCCTCAGGCCGCCCCAGACCCAAAGGCCAACTTCTCCACCAATAACAGCAACCCAGGGGCACGGC
CACCATCCCTGAGGGCCACGGCTCGGCTGCCACAGAGGGGCTGCCCAGGGGACGGGCAAGAGGCTGCTCG
GCCAGCAGACAAGGTCCAGATGGTGGAGCAGCTCTTTGGGCTGGGTCTTCGGCCACGGGTGGAGTGCCAC
AAGGTCCTAGAGATGTTCGACTGGAACCTAGAGCAAGCCGGCTGTCACCTTCTGGGCTCCTGTGGCCCTG
CTCATCACAAACGC


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
>MR230345 representing NM_001289443
Red=Cloning site Green=Tags(s)

MDPPDLLSVELSTSRPTQHLGRVKKPTYDPVSEDPDPLSSDFKRLGLRKPALPRGLWLAKPSARVPGTKA
DRSSGGEVTLIDFGEEPVVPTPRPCAPSLAQLAMDACSLLDKTPPQSPTRALPRPLHPTPVVDWDARPLP
PPPAYDDVAQDEDDFEVCSINSTLVGAGLPAGPSQGETNYAFVPEQAQMPPALEDNLFLPPQGGGKPPSS
VQTAEIFQALQQECMRQLQVPTGQLTPSPTPGGDDKPQVPPRVPIPPRPTRPRVELSPAPSGEEETSRWP
GPASPPRVPPREPLSPQGSRTPSPLVPPGSSPLPHRLSSSPGKTMPTTQSFASDPKYATPQVIQAPGPRA
GPCILPIVRDGRKVSSTHYYLLPERPPYLERYQRFLREAQSPEEPAALPVPPLLPPPSTPAPAAPTATVR
PMPQAAPDPKANFSTNNSNPGARPPSLRATARLPQRGCPGDGQEAARPADKVQMVEQLFGLGLRPRVECH
KVLEMFDWNLEQAGCHLLGSCGPAHHKR

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001289443
ORF Size 1554 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001289443.1, NP_001276372.1
RefSeq Size 2721 bp
RefSeq ORF 1557 bp
Locus ID 51789
UniProt ID O54967
Cytogenetics 16 B3
MW 56 kDa
Gene Summary Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors. Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes. Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. May be involved both in adult synaptic function and plasticity and in brain development. Activates AKT1 by phosphorylating it on 'Tyr-176'. Phosphorylates AR on 'Tyr-267' and 'Tyr-363' thereby promoting its recruitment to androgen-responsive enhancers (AREs). Phosphorylates WWOX on 'Tyr-287'. Phosphorylates MCF2, thereby enhancing its activity as a guanine nucleotide exchange factor (GEF) toward Rho family proteins. Contributes to the control of AXL receptor levels. Confers metastatic properties on cancer cells and promotes tumor growth by negatively regulating tumor suppressor such as WWOX and positively regulating pro-survival factors such as AKT1 and AR.[UniProtKB/Swiss-Prot Function]

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